1887

Abstract

A repetitive element from the genome of serovar type (‘’) was identified, cloned and sequenced. Similar sequences were shown by hybridization to be encoded by a further eight of 32 other leptospiral serovars tested. An undefined number of repetitive elements were located in the genome; sequence degeneracy of the elements was observed and no significant open reading frames were identified within the AT-rich (60%) 1467 bp repetitive element. The termini encoded a GC-rich 8 bp repeat motif and two variants showed rearrangements centred on these motifs. The nucleotide sequences of the chromosomal regions flanking the repetitive elements were determined but showed no similarities, with one exception which had a GAAC repeat directly adjacent to both termini. Similar hybridization patterns were shown by Southern transfers of total genomic digests probed with the repetitive element. Oligonucleotide primer pairs designed from sequences internal to the repetitive element and adjacent chromosomal regions were used in polymerase chain reaction experiments. With one primer pair all isolates, but no other serovar, gave identical amplified products. Evidence that the repetitive element may have derived from an acquired insertion sequence that is now inactive and chromosomally fixed is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-5-1101
1991-05-01
2021-10-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/5/mic-137-5-1101.html?itemId=/content/journal/micro/10.1099/00221287-137-5-1101&mimeType=html&fmt=ahah

References

  1. Berg D. E. 1980; Control of gene expression by a mobile genetic recombinational switch. Proceedings of the National Academy of Sciences of the United States of America 774880–4884
    [Google Scholar]
  2. Calos M. P., Miller J. H. 1980; Transposable elements. Cell 20:579–595
    [Google Scholar]
  3. Chappel R. J., Millar B. D., Adler B., Hill J., Jeffers M. J., Jones R. T., McCaughan C. J., Mead L. J., Silbreck N. W. 1989; Leptospira interrogans serovarhardjo is not a major cause of bovine abortion in Victoria. Australian Veterinary Journal 66:330–333
    [Google Scholar]
  4. Cole J. R. 1984; Spirochetes. Diagnostic Procedures in Veterinary Bacteriology and Mycology, 4.40–65 Carter G. R. Springfield, Illinois: Charles C. Thomas Publishing;
    [Google Scholar]
  5. Ellis W. A. 1986; The diagnosis of leptospirosis in farm animals. The Present State of Leptospirosis Diagnosis and Control13–31 Ellis W. A., Little T. W. A. Dordrecht, The Netherlands: Martinus Nijhoff;
    [Google Scholar]
  6. Ellis W. A., O’Brien J. J., Neill S. D. 1982; Bovine leptospirosis: microbiological and serological findings in aborted fetuses. Veterinary Record 110:147–150
    [Google Scholar]
  7. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  8. Hata K., Yamaguchi T., Ono E., Yanagawa R. 1988; Comparative analysis of leptospiral strains ictero number 1 and RGA by restriction endonuclease DNA analysis. Japanese Journal of Veterinary Research 36:133–136
    [Google Scholar]
  9. Hefron F. 1983; Tn3 and its relatives. Mobile Genetic Elements217–262 Shapiro S. L. London: Academic Press;
    [Google Scholar]
  10. Johnson R. C., Faine S. 1984; Leptospiraceae. Berger's Manual of Systematic Bacteriology 162–67 Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  11. Kleckner N. 1979; DNA sequence analysis of Tn10 insertions: origin and role of 9 bp flanking repetitions during TN10 translocation. Cell 16:711–720
    [Google Scholar]
  12. Lecour H., Miranda M., Magro C., Rocha A., Goncalves V. 1989; Human leptospirosis - a review of 50 cases. Infection 17:8–12
    [Google Scholar]
  13. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Marshall R. B., Wilton B. E., Robinson A. J. 1981; Identification of Leptospira serovars by restriction-endonuclease analysis. Journal of Medical Microbiology 14:163–166
    [Google Scholar]
  15. Nielsen J. N., Armstrong C. H., Nielsen N. C. 1989; Relationships among selectedLeptospira interrogans serogroups as determined by nucleic acid hybridisation. Journal of Clinical Microbiology 27:2724–2729
    [Google Scholar]
  16. Ohtsubo H., Ohtsubo E. 1977; Repeated DNA sequences in plasmids, phages and chromosomes. DNA Insertion Elements, Plasmids and Episomes49–64 Bukhari A. I., Shapiro J. A., Adhya S. L. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Robinson A. J., Ramadass P., Lee A., Marshall R. B. 1982; Differentiation of subtypes withInLeptospira interrogans serovarsHardjo Balcanica andTarassovi by BRENDA. Journal of Medical Microbiology 15:331–338
    [Google Scholar]
  18. Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. 1985; Enzymatic amplification ofβ- globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350–1354
    [Google Scholar]
  19. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Silbreck N. W., Davies W. D. 1989; Restriction endonuclease analysis of Australian isolates ofLeptospira interrogans serovarhardjo. Australian Veterinary Journal 66:183–184
    [Google Scholar]
  21. Terpstra W. J., Schoone G. J., Ter Schegget J. 1986; Detection of leptospiral DNA by nucleic acid hybridization with32P-and biotin-labelled probes. Journal of Medical Microbiology 22:23–28
    [Google Scholar]
  22. Thiermann A. B. 1984; Leptospirosis: current development and trends. Journal of the American Veterinary Medical Association 184:722–725
    [Google Scholar]
  23. Thiermann A. B., Handsaker J. W., Foley J. W., Kinscote B. 1986; Reclassification of leptospiral isolates belonging to serogroups Mini and Sejroe by restriction endonuclease analysis. American Journal of Veterinary Research 47:61–66
    [Google Scholar]
  24. Van Eys G. J. J. M., Zaal J., Schoone G. J. 1988; DNA hybridization with hardjobovis-specific recombinant probes as a method for type discrimination ofLeptospira interrogans serovarhardjo. Journal of General Microbiology 134:567–574
    [Google Scholar]
  25. Van Eys G. J. J. M., Gravenkamp C., Gerritsen M. J., Quint W., Cornelissen M. T. E., Ter Schegget J., Terpstra W. J. 1989; Detection of Leptospires in urine by Polymerase Chain Reaction. Journal of Clinical Microbiology 27:2258–2262
    [Google Scholar]
  26. Zainuddin Z., Dale J. W. 1989; Polymorphic repetitive DNA sequences InMycobacterium tuberculosis detected with a gene probe from aMycobacterium fortuitum plasmid. Journal of General Microbiology 135:2347–2355
    [Google Scholar]
  27. Zuerner R. L., Bolin C. A. 1988; Repetitive sequence element cloned fromLeptospira interrogans serovarhardjo typehardjo-bovis provides a sensitive diagnostic probe for bovine leptospirosis. Journal of Clinical Microbiology 25:2236–2238
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-5-1101
Loading
/content/journal/micro/10.1099/00221287-137-5-1101
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error