1887

Abstract

Toxigenic strains of produce the 147 kDa protein toxin (PMT) which is responsible for the osteoclastic bone resorption in progressive atrophic rhinitis in pigs and induces such resorption in all experimental animals tested so far. In the present study we have carried out immunocytochemistry on formaldehyde- and glutaraldehyde-fixed ultracryocut using a pool of monoclonal antibodies against different epitopes on PMT as the first layer and affinity purified rabbit anti-mouse IgG as the second layer. Goat anti-rabbit IgG conjugated with 5 nm gold particles was used as marker. The gold particles were silver-enhanced prior to examination in the transmission electron microscope. Whole bacteria were also immunostained after fixation and critical point drying and examined by scanning transmission electron microscopy. The results showed that PMT was located in the cytoplasm of by scanning electron microscopy. Neither pili nor flagella could be detected on the surface of the negatively stained strains investigated. PMT has a series of characteristics encompassed in the definition of an exotoxin. However, that PMT was not secreted by living intact is unexpected for an exotoxin.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-5-1067
1991-05-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/5/mic-137-5-1067.html?itemId=/content/journal/micro/10.1099/00221287-137-5-1067&mimeType=html&fmt=ahah

References

  1. Bastholm L., Scopsi L., Nielsen M. H. 1986; Silver-enhanced immunogold staining of semithin and ultrathin cryosections. Journal of Electron Microscopy Technique 4:175–176
    [Google Scholar]
  2. Braude A. I., Davis C. E., Fierer J. 1986 Infectious Diseases and Medical Microbiology42–59 Philadelphia: W. B. Saunders Company;
    [Google Scholar]
  3. Elling F., Pedersen K. B. 1985; The pathogenesis of persistent turbinate atrophy induced by toxigenic Pasteurella multocida in pigs. Veterinary Patholology 22:469–474
    [Google Scholar]
  4. Foged N. T., Pedersen K. B., Elling F. 1987; Characterization and biological effects of the Pasteurella multocida toxin. FEMS Microbiology Letters 43:45–51
    [Google Scholar]
  5. Foged N. T. 1988; Quantitation and purification of the Pasteurella multocida toxin by using monoclonal antibodies. Infection and Immunity 56:1901–1906
    [Google Scholar]
  6. Günther H., Rosner H., Godat M., Erler W. 1986; Electron microscopic visualization of the capsule of Pasteurella. Acta histochemica, Suppl. Band XXXIII:293–296
    [Google Scholar]
  7. Jawetz E., Melnick J. L., Adelberg E. A., Brooks G. F., Butel J. S., Ornston L. N. 1989 Medical Microbiology128–129 New York: Prentice-Hall;
    [Google Scholar]
  8. Nakai T., Sawata A., Tsuji M., Kume K. 1984; Purification of dermonecrotic toxin from a sonic extract of Pasteurella multocida SP-72 serotype D. Infection and Immunity 46:429–434
    [Google Scholar]
  9. Nakai T., Kume K., Yoskikawa H., Oyamada T., Yoshikawa T. 1988; Adherence of Pasteurella multocida or Bordetella bronchisep- tica to the swine nasal epithelial cell in vitro. Infection and Immunity 56:234–240
    [Google Scholar]
  10. Nakai T., Sawata A., Kume K. 1985; Intracellular locations of dermonecrotic toxins in Pasteurella multocida and in Bordetella bronchiseptica. American Journal of Veterinary Research 46:4
    [Google Scholar]
  11. Pedersen K. B., Elling F. 1984; The pathogenesis of atrophic rhinitis in pigs induced by toxigenic Pasteurella multocida. Journal of Comparative Pathology 94:203–214
    [Google Scholar]
  12. Scopsi L., Larsson L.-I., Bastholm L., Nielsen M. N. 1986; Silver-enhanced colloidal gold probes as markers for scanning electron microscopy. Histochemistry 86:35–41
    [Google Scholar]
  13. Tokuyasu K. T. 1986; Application of cryoultramicrotomy to immunocytochemistry. Journal of Microscopy 143:139–149
    [Google Scholar]
  14. Trigo E., Pijoan C. 1988; Presence of pili in Pasteurella multocida strains associated with atrophic rhinitis. Veterinary Record 122:19
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-5-1067
Loading
/content/journal/micro/10.1099/00221287-137-5-1067
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error