When grown on two agar media in Petri dishes a Sacc. isolate produced sclerotia in different patterns, predominantly at the colony centre on one medium (glucose/yeast extract) but at the dish wall on the other (glucose/ammonium nitrate). However, on both media a short cold treatment (3 h at 5 °C) of colonies grown at 24 °C temporarily interrupted growth and induced subsequent sclerotial formation in a ring where the colony margin was at the time of the cold shock. A second cold treatment 1 d after the first induced a sclerotial ring but negated the effect of the first treatment. The results support the view that interruption of hyphal growth triggers sclerotial formation.


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