Antigenic cell wall mannoproteins in isolates and in other species Free

Abstract

Polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs), raised against mannoprotein components from ATCC 26555 (serotype A) blastoconidia and mycelial cell walls, were used to investigate antigenic similarities among wall mannoproteins from other serotype A and B strains, and from and . Radioactively labelled walls isolated from cells grown at either 28 °C or 37 °C were digested with a β-glucanase complex (Zymolyase 20T) to release cell-wall-bound mannoproteins. Numerous molecular species with different electrophoretic mobilities were released from the various isolates. Differences appeared to be related to both the organism and the growth temperature. Among the major protein components solubilized were mannoproteins larger than 100 kDa (high molecular mass mannoproteins), heterogeneous in size in most cases. Antigenic homology was detected among the cell wall high molecular mass mannoproteins of the two serotype A isolates, whereas significant qualitative and quantitative differences were detected between serotype A and serotype B cell-wall-bound antigenic profiles. Moreover, and wall antigenic determinants were not recognized by the preparations of pAbs and mAbs raised against walls. A mannoprotein with a molecular mass of 33-34 kDa was present in the enzymic wall digests of all the organisms studied. When probed with pAbs raised against the protein moiety of the 33 kDa cell wall mannoprotein of , antigenic cross-reactivity was observed in all cases except . There appear to be significant antigenic differences between the mannoproteins of different isolates of , and between those of and other species.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-5-1053
1991-05-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/5/mic-137-5-1053.html?itemId=/content/journal/micro/10.1099/00221287-137-5-1053&mimeType=html&fmt=ahah

References

  1. Bodey G. 1984; Candidiasis: agrowing concern. American Journal of Medicine 77:1–48
    [Google Scholar]
  2. Brawner D. L., Cutler J. E. 1984; Variability in expression of a cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody. Infection and Immunity 43:966–972
    [Google Scholar]
  3. Brawner D. L., Cutler J. E. 1986a; Ultrastructural and biochemical studies of two dynamically expressed cell surface determinants on Candida albicans. Infection and Immunity 51:327–336
    [Google Scholar]
  4. Brawner D. L., Cutler J. E. 1986b; Variability in expression of cell surface antigens of Candida albicans during morphogenesis. Infection and Immunity 51:337–343
    [Google Scholar]
  5. Brawner D. L., Cutler J. E., Beatty W. L. 1990; Caveats in the investigation of form-specific molecules of Candida albicans. Infection and Immunity 58:378–383
    [Google Scholar]
  6. Burnette W. N. 1981; “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  7. Casanova M., Gil M. L., Cardeñoso L., Martínez J. P., Sentandreu R. 1989; Identification of wall-specific antigens synthesized during germ tube formation by Candida albicans. Infection and Immunity 57:262–271
    [Google Scholar]
  8. Casanova M., Martínez J. P., Chaffin W. L. 1990; Fab fragments from a monoclonal antibody against a germ tube specific mannoprotein block yeast-to-mycelium transition in Candida albicans. Infection and Immunity 58:3810–3812
    [Google Scholar]
  9. Chaffin W. L., Skudlarek J., Morrow K. J. 1988; Variable expression of a surface determinant during proliferation of Candida albicans. Infection and Immunity 56:302–309
    [Google Scholar]
  10. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  11. Elorza M. V., Rico H., Sentandreu R. 1983; Cell wall composition and protoplast regeneration in Candida albicans. Antonie van Leeuwenhoek 49:457–469
    [Google Scholar]
  12. Elorza M. V., Murgui A., Sentandreu R. 1985; Dimorphism in Candida albicans : contribution of mannoproteins to the architecture of yeast and mycelial cell walls. Journal of General Microbiology 131:2209–2216
    [Google Scholar]
  13. Elorza M. V., Mormeneo M., Garcia De La Cruz F., Gimeno C., Sentandreu R. 1989; Evidence for the formation of covalent bonds between macromolecules in the domain of the wall of Candida albicans mycelial cells. Biochemical and Biophysical Research Communications 162:1118–1125
    [Google Scholar]
  14. Hawkes R. 1982; Identification of concanavalin A-binding proteins after sodium dodecyl sulfate-gel electrophoresis and protein blotting. Analytical Biochemistry 123:143–146
    [Google Scholar]
  15. Herrero E., Boyd A. 1986; Mannoproteins from the cell wall of Kluyveromyces lactis. FEMS Microbiology Letters 36:207–211
    [Google Scholar]
  16. Herrero E., Sanz P., Sentandreu R. 1987; Cell wall proteins liberated by Zymolyase from several ascomycetous and imperfect yeasts. Journal of General Microbiology 133:2895–2903
    [Google Scholar]
  17. Hopwood V., Poulain D., Fortier B., Evans G., Vernes A. 1986; A monoclonal antibody to a cell wall component of Candida albicans. Infection and Immunity 54:222–227
    [Google Scholar]
  18. Kumar B. V., Medoff G., Kobayashi G. S., Sieling W. L. 1985; Cross-reacting human and rabbit antibodies to antigens of Histoplasma capsulatum, Candida albicans, and Saccharomyces cerevisiae. Infection and Immunity 48:806–812
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. Lee K. L., Buckley H. R., Campbell C. C. 1975; An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans. Sabouraudia 13:148–153
    [Google Scholar]
  21. Martínez J. P., Gil M. L., Casanova M., Lopez-Ribot J. L., Garcia De Lomas J., Sentandreu R. 1990; Wall mannoproteins in cells from colonial phenotypic variants of Candida albicans. Journal of General Microbiology 136:2421–2432
    [Google Scholar]
  22. Meunier F. 1989; Candidiasis. European Journal of Clinical Microbiology and Infectious Diseases 8:438–447
    [Google Scholar]
  23. Millette C. F., Scott B. K. 1984; Identification of spermatogenic cell plasma membrane glycoproteins by two dimensional electrophoresis and lectin blotting. Journal of Cell Science 65:233–248
    [Google Scholar]
  24. Miyakawa Y., Kagaya K., Fukazawa Y., Soe G. 1986; Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans. Journal of Clinical Microbiology 23:881–886
    [Google Scholar]
  25. Odds F. C. 1988 Candida and Candidosis London: Bailliere-Tindall;
    [Google Scholar]
  26. Pastor F. I. J., Valentin E., Herrero E., Sentandreu R. 1984; Structure of the Saccharomyces cerevisiae cell wall: mannoproteins released by Zymolyase and their contribution to wall architecture. Biochimica et Biophysica Acta 802:292–300
    [Google Scholar]
  27. Ponton J., Jones M. 1986; Analysis of cell wall extracts of Candida albicans by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot techniques. Infection and Immunity 53:565–572
    [Google Scholar]
  28. Poulain D., Hopwood V., Vernes A. 1985; Antigenic variability of Candida albicans. Critical Reviews in Microbiology 12:223–270
    [Google Scholar]
  29. Sanz P., Herrero E., Sentandreu R. 1987; Secretory pattern of a major integral mannoprotein of the yeast cell wall. Biochimica et Biophysica Acta 924:193–203
    [Google Scholar]
  30. Slutsky B., Buffo J., Soll D. R. 1985; High-frequency “switching” of colony morphology in Candida albicans. Science 230:666–669
    [Google Scholar]
  31. Sundstrom P. M., Kenny G. E. 1985; Enzymatic release of germtube specific antigens from cell walls of Candida albicans. Infection and Immunity 49:609–614
    [Google Scholar]
  32. Sundstrom P. M., Nichols E. J., Kenny G. E. 1987; Antigenic differences between mannoproteins of germ tubes and blastospores of Candida albicans. Infection and Immunity 55:616–620
    [Google Scholar]
  33. Sundstrom P. M., Tam M. R., Nichols E. J., Kenny G. E. 1988; Antigenic differences in the surface mannoproteins of Candida albicans as revealed by monoclonal antibodies. Infection and Immunity 56:601–606
    [Google Scholar]
  34. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedinqs of the National Academy of Sciences of the United States of America 764350–4354
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-5-1053
Loading
/content/journal/micro/10.1099/00221287-137-5-1053
Loading

Data & Media loading...

Most cited Most Cited RSS feed