1887

Abstract

Summary: The resistance spectrum to bacteriophase ø3T of different 168/W23 strains hybrid for wall teichoic acids suggested that poly(3--β--glucopyranosyl-N-acetylgalactosamine 1-phosphate), a so-called minor teichoic acid of strain 168, forms part of the receptor for this phage, and a serologically related group of phages. A representative sample of 25 mutants specifically resistant to ø3T, obtained from a mutagenized culture by direct selection, were all found to have a greatly reduced galactosamine content. Relevant mutations in these strains were shown by PBS1 transduction and transformation to belong to two linkage groups; a minority, associated with an atypical colony morphology, were localized between and , wherease the majority mapped between and (formerly ), a region containing all known genes involved in the synthesis of the major wall teichoic acid, poly(glycerol phosphate). The fomer mutations mapped in a new locus, , characterized by a deficiency in UDP-N-acetylglucosamine 4-epimerase, while the latter ones, as well as the previously identified -3 ( Estrela , 1986 , 132, 411–415), map in a lucus named . They are likely to affect membrane-bound enzymes involved in the synthesis of the galactosamine-containing teichoic acid. A possible biological role of this polymer is discussed.

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1991-04-01
2021-07-27
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