1887

Abstract

Summary: 3AI-generated DNA fragments of the large plasmid encoding the form I antigen were cloned into with cosmid vector pHSG262. One resulting plasmid, designated pJK1137, was studied further. Restriction endonuclease mapping and analysis of transposon Tn insertion mutants demonstrated that the form I antigen genes were located within a region of about 12·6 kb consisting of the two contiguous III fragments of 1·26 kb and 12·4 kb. The results of complementation studies between Tn insertion mutants of pJK1137 and recombinant plasmids carrying different parts of the form I antigen genes indicated that the 12·6 kb DNA sequence contained at least four gene clusters, regions A, B, C and D. Analysis of radioactively labelled proteins in minicells demonstrated that the DNA sequence of about 12·6 kb coded for at least four specific proteins of 42, 23, 48 and 39 kDa. The former two were coded by region A, the latter two by region D.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-4-867
1991-04-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/4/mic-137-4-867.html?itemId=/content/journal/micro/10.1099/00221287-137-4-867&mimeType=html&fmt=ahah

References

  1. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  2. Boyer H. W., Roulland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA in Esherichia coli. Journal of Molecular Biology 41:459–472
    [Google Scholar]
  3. Brady G., Jantzen H. M., Bernard H. U., Brown R., Schütz G., Hashimoto-Gotoh T. 1984; New cosmid vectors developed for eukaryotic DNA cloning. Gene 27:223–232
    [Google Scholar]
  4. Chang A. C. Y., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
    [Google Scholar]
  5. DuPont H. L., Hornick R. B., Dawkins A. T., Snyder M. J., Formal S. B. 1969; The response of man to virulent Shigella flexneri 2a. Journal of Infectious Diseases 119:296–299
    [Google Scholar]
  6. Formal S. B., Kent T. H., May H. C., Palmer A., Falkow S., LaBrec E. H. 1966; Protection of monkeys against experimental shigellosis with a living attenuated oral polyvalent dysentery vaccine. Journal of Bacteriology 92:17–22
    [Google Scholar]
  7. Gamian A., Romanowska E. 1982; The core structure of Shigella sonnei lipopolysaccharide and the linkage between O-specific polysaccharide and the core region. European Journal of Biochemistry 129:105–109
    [Google Scholar]
  8. Harayama S., Hazelbauer L. 1982; A gene coding for a periplasmic protein is located near the locus for termination of chromosome replication in Escherichia coli. Journal of Bacteriology 151:1391–1396
    [Google Scholar]
  9. Hashimoto-Gotoh T., Franklin F. C. H., Nordheim A., Timmis K. N. 1981; Specific-purpose plasmid cloning vectors. I. Low copy number, temperature-sensitive, mobilization-defective pSC101-derived containment vectors. Gene 16:227–235
    [Google Scholar]
  10. Hitchcock P. J., Brown T. M. 1983; Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. Journal of Bacteriology 154:269–277
    [Google Scholar]
  11. Kado C. I., Liu S. T. 1981; Rapid procedure for detection and isolation of large and small plasmids. Journal of Bacteriology 145:1365–1373
    [Google Scholar]
  12. Kato J., Suzuki H., Hirota Y. 1984; Overlapping of the coding regions for a and y components of penicillin-binding protein lb in Escherichia coli. Molecular and General Genetics 196:449–457
    [Google Scholar]
  13. Kenne L., Lindberg B., Peîersson K., Katzenellenbogen E., Romanowska E. 1980; Structural studies of the O-specific side-chains of the Shigella sonnei phase I lipopolysaccharide. Carbohydrate Research 78:119–126
    [Google Scholar]
  14. Kopecko D. J., Washington O., Formal S. B. 1980; Genetic and physical evidence for plasmid control of Shigella sonnei form I cell surface antigen. Infection and Immunity 29:207–214
    [Google Scholar]
  15. LaBrec E. H., Schneider H., Magnani T. J., Formal S. B. 1964; Epithelial cell penetration as an essential step in the pathogenesis of bacillary dysentery. Journal of Bacteriology 88:1503–1518
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  17. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage PI. Virology 1:190–206
    [Google Scholar]
  18. Mandel M., Higa A. 1970; Calcium-dependent bacteriophage DNA infection. Journal of Molecular Biology 53:159–162
    [Google Scholar]
  19. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  20. Mel D. M., Terzin A. L., Vuksic L. 1965; Studies on vaccination against bacillary dysentery. 3. Effective oral immunization against Shigella flexneri 2a in a field trial. Bulletin of the World Health Organization 32:647–655
    [Google Scholar]
  21. Okamura N., Nagai T., Nakaya R., Kondo S., Murakami M., Hisatsune K. 1983; HeLa cell invasiveness and O antigen of Shigella flexneri as separate and prerequisite attributes of virulence to evoke keratoconjunctivitis in guinea pigs. Infection and Immunity 39:505–513
    [Google Scholar]
  22. Riley L. W., Junio L. N., Libaek L. B., Schoolnik G. K. 1987; Plasmid-encoded expression of lipopolysaccharide O-antigenic polysaccharide in enteropathogenic Escherichia coli. Infection and Immunity 55:2052–2056
    [Google Scholar]
  23. Sansonetti P. J., Kopecko D. J., Formal S. B. 1981; Shigella sonnei plasmids: evidence that a large plasmid is necessary for virulence. Infection and Immunity 34:75–83
    [Google Scholar]
  24. Sansonetti P. J., Kopecko D. J., Formal S. B. 1982; Involvement of a plasmid in the invasive ability of Shigella flexneri. Infection and Immunity 35:852–860
    [Google Scholar]
  25. Sansonetti P. J., Hale T. L., Dammin G. J., Kapfer C., Collins H. H. Jr, Formal S. B. 1983; Alterations in the pathogenicity of Escherichia coli K-12 after transfer of plasmid and chromosomal genes from Shigella flexneri. Infection and Immunity 39:1392–1402
    [Google Scholar]
  26. Watanabe H., Nakamura A. 1985; Large plasmids associated with virulence in Shigella species have a common function necessary for epithelial cell penetration. Infection and Immunity 48:260–262
    [Google Scholar]
  27. Watanabe H., Nakamura A. 1986; Identification of Shigella sonnei form I plasmid genes necessary for cell invasion and their conservation among Shigella species and enteroinvasive Escherichia coli. Infection and Immunity 53:352–358
    [Google Scholar]
  28. Weislander L. 1979; A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Analytical Biochemistry 98:305–309
    [Google Scholar]
  29. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains : nucleotide sequences of the M13 mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  30. Yoshimori R., Roulland-Dussoix D., Boyer H. W. 1972; R factor-controlled restriction and modification of deoxyribonucleic acids: restriction mutants. Journal of Bacteriology 112:1275–1279
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-4-867
Loading
/content/journal/micro/10.1099/00221287-137-4-867
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error