1887

Abstract

Summary: Six strains of were grown in defined medium which had been deferrated by ion-exchange chromatography and then supplemented with FeCl to give iron concentrations ranging from 0·026 μ to 0·8 μ. Growth in 0-026 μM-iron (measured as increase in biomass) was reduced by 26–59% as compared with that in excess (0·8 μM)iron. With five of the strains, adhesion to buccal epithelial cells was maximal after growth in 0·2–0-4μM-iron, but strain GDH 2023 adhered best when grown in 0·026 μ-iron. Differences in yeast cell-wall composition were revealed by Zymolyase treatment of whole cells and by I-labelling of surface proteins. SDS-PAGE of iodinated proteins, followed by autoradiography, showed quantitative but no qualitative differences in protein profiles of iron-deficien and iron-replete organisms. The ability of all strains to form germ tubes in serum was near-maximal after growth in 0·2–0·4 μ-iron but was inhibited by up to 93% following growth in lower results indicate that expression of important virulence attributes by is highly iron and that expression may therefore be significantly different from that observed under conventional laboratory conditions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-4-859
1991-04-01
2021-09-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/4/mic-137-4-859.html?itemId=/content/journal/micro/10.1099/00221287-137-4-859&mimeType=html&fmt=ahah

References

  1. Bedell G. W., Anderson R. V. 1985; Inhibition of the differentiation of Candida albicans by the chelator 1,10-phenanthroline. Mycopathologia 92:161–167
    [Google Scholar]
  2. Bezkorovainy A. 1987; Iron proteins. Iron and Infection. Molecular, Physiological and Clinical Aspects,27–67 Bullen J. J., Griffiths E. Chichester: John Wiley & Sons;
    [Google Scholar]
  3. Brown M. R. W., Williams P. 1985; The influence of environment on envelope properties affecting survival of bacteria in infections. Annual Review of Microbiology 39:527–556
    [Google Scholar]
  4. Bullen J. J., Griffiths E. 1987 Iron and Infection. Molecular, Physiological and Clinical Aspects Chichester: John Wiley & Sons;
    [Google Scholar]
  5. Critchley I. A., Douglas L. J. 1987; Role of glycosides as epithelial cell receptors for Candida albicans. Journal of General Microbiology 133:637–643
    [Google Scholar]
  6. Douglas L. J. 1987; Adhesion of Candida species to epithelial surfaces. CRC Critical Reviews in Microbiology 15:27–43
    [Google Scholar]
  7. Douglas L. J., Houston J. G., McCourtie J. 1981; Adherence of Candida albicans to human buccal epithelial cells after growth on different carbon sources. FEMS Microbiology Letters 12:241–243
    [Google Scholar]
  8. Finkelstein R. A., Sciortino C. V., McIntosh M. A. 1983; Role of iron in microbe-host interactions. Reviews of Infectious Diseases 5:S759–S777
    [Google Scholar]
  9. Holmes R. K., Russell L. M. 1983; Role of iron in regulating synthesis of bacterial toxins. Microbiology 1983,359–362 Schlessinger D. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Holzberg M., Artis W. M. 1983; Hydroxamate siderophore production by opportunistic and systemic fungal pathogens. Infection and Immunity 40:1134–1139
    [Google Scholar]
  11. Ismail A., Bedell G. W., Lupan D. M. 1985; Siderophore production by the pathogenic yeast, Candida albicans. Biochemical and Biophysical Research Communications 130:885–891
    [Google Scholar]
  12. Kadurugamuwa J. L., Anwar H., Brown M. R. W., Shand G. H., Ward K. H. 1987; Media for study of growth kinetics and envelope properties of iron-deprived bacteria. Journal of Clinical Microbiology 25:849–855
    [Google Scholar]
  13. Kirkpatrick C. H., Green I., Rich R. R., Schade A. L. 1971; Inhibition of growth of Candida albicans by iron-unsaturated lactoferrin: relation to host-defense mechanisms in chronic mucocutaneous candidiasis. Journal of Infectious Diseases 124:539–544
    [Google Scholar]
  14. McCourtie J., Douglas L. J. 1984; Relationship between cell surface composition, adherence and virulence of Candida albicans. Infection and Immunity 45:6–12
    [Google Scholar]
  15. Matthews R., Burnie J. P. 1988; Diagnosis of systemic candidiasis by an enzyme-linked dot immunobinding assay for a circulating immunodominant 47-kilodalton antigen. Journal of Clinical Microbiology 26:459–463
    [Google Scholar]
  16. Matthews R., Wells C., Burnie J. P. 1988; Characterisation and cellular localisation of the immunodominant 47-Kda antigen of Candida albicans. Journal of Medical Microbiology 27:227–232
    [Google Scholar]
  17. Neilands J. B. 1981; Microbial iron compounds. Annual Review of Biochemistry 50:715–731
    [Google Scholar]
  18. Neilands J. B. 1982; Microbial envelope proteins related to iron. Annual Review of Microbiology 36:285–309
    [Google Scholar]
  19. Odds F. C. 1988 Candida and Candidosis, 2. London: Baillière Tindall;
    [Google Scholar]
  20. Page S., Odds F. C. 1988; Binding of plasma proteins to Candida species in vitro. Journal of General Microbiology 134:2693–2702
    [Google Scholar]
  21. Paul T. R., Smith S. N., Brown M. R. W. 1989; Effect of iron depletion on cell-wall antigens of Candida albicans. Journal of Medical Microbiology 28:93–100
    [Google Scholar]
  22. Shiraishi A., Arai T. 1979; Antifungal activity of transferrin. Sabouraudia 17:79–83
    [Google Scholar]
  23. Soll D. R. 1985; The role of zinc in Candida dimorphism. Current Topics in Medical Mycology 1258–285 McGinnis M. R. New York: Springer-Verlag;
    [Google Scholar]
  24. Valenti P., Visca P., Antonini G., Orsi N. 1986; Interaction between lactoferrin and ovotransferrin and Candida cells. FEMS Microbiology Letters 33:271–275
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-4-859
Loading
/content/journal/micro/10.1099/00221287-137-4-859
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error