1887

Abstract

Summary: Six strains of were grown in defined medium which had been deferrated by ion-exchange chromatography and then supplemented with FeCl to give iron concentrations ranging from 0·026 μ to 0·8 μ. Growth in 0-026 μM-iron (measured as increase in biomass) was reduced by 26–59% as compared with that in excess (0·8 μM)iron. With five of the strains, adhesion to buccal epithelial cells was maximal after growth in 0·2–0-4μM-iron, but strain GDH 2023 adhered best when grown in 0·026 μ-iron. Differences in yeast cell-wall composition were revealed by Zymolyase treatment of whole cells and by I-labelling of surface proteins. SDS-PAGE of iodinated proteins, followed by autoradiography, showed quantitative but no qualitative differences in protein profiles of iron-deficien and iron-replete organisms. The ability of all strains to form germ tubes in serum was near-maximal after growth in 0·2–0·4 μ-iron but was inhibited by up to 93% following growth in lower results indicate that expression of important virulence attributes by is highly iron and that expression may therefore be significantly different from that observed under conventional laboratory conditions.

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/content/journal/micro/10.1099/00221287-137-4-859
1991-04-01
2019-11-18
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-4-859
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