Summary: An mRNA-dependent cell-free translation system has been developed from the human pathogenic fungus using either S30 or S100 lysates prepared from glass-bead-disrupted whole cells. Translation of the synthetic template poly(U) in this system is highly efficient at temperatures up to 37 °C and is ATP-dependent. Studies using a range of elongation-specific inhibitors suggest that the mechanism of translational elongation in is similar to that of another yeast, . A micrococcal-nuclease-treated S100 lysate was able to translate exogenously-supplied homologous mRNAs, and a range of heterologous natural mRNAs, using an initiation mechanism that is inhibited by the antibiotic edeine and the 5′ cap analogue 7-methylguanosine 5′-monophosphate (mGMP). As with cell-free lysates prepared from , the lysate is unable to initiate translation upon natural mRNAs at temperatures above 20 °C.


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