1887

Abstract

Isoelectric focusing was used to compare the complement of phosphoglucose isomerase isoenzymes in a wild-type strain of and in a strain with a deletion in the structural gene. Deletion of the gene did not result in the absence of the high- isoenzyme I but the low- isoenzyme II was absent. Hence, the isoenzymes must be the products of two genes. If were the sole structural gene its deletion would result in the disappearance of both isoenzymes. After a temperature shift-up a -bearing strain had cell cycle arrested and contained only 8% of the polysaccharide in the wild-type. Phosphoglucose isomerase is required for the synthesis of fructose 6-phosphate (F6-P), a precursor of the cell wall components chitin and mannoprotein (‘mannan’), which are a polysaccharide and contain polysaccharide, respectively. Since the mutation confers a temperature-sensitive phosphoglucose isomerase, the likely explanation for cell cycle arrest caused by this mutation is that the defective phosphoglucose isomerase results in a reduction of F6-P and hence an inability to synthesize the mannan and chitin needed for cytokinesis and cell separation. Revertants of a bearing strain were selected for their ability to grow on glucose at 25 °C and this yielded a number of different phenotypes. Amongst the isolates was a strain which had undergone an intragenic reversion at the locus, designated . This mutation permits growth and cell division at 25 °C but results in cell cycle arrest at 36 °C. These results are all consistent with the notion that in there are two genes ( and ) which encode the low- and high- isoenzymes, respectively, of phosphoglucose isomerase and that the two gene products physically interact for activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-4-765
1991-04-01
2021-05-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/4/mic-137-4-765.html?itemId=/content/journal/micro/10.1099/00221287-137-4-765&mimeType=html&fmt=ahah

References

  1. Aguilera A. 1986; Deletion of the phosphoglucose isomerase structural gene makes growth and sporulation glucose dependent in Saccharomyces cerevisiae . Molecular and General Genetics 204:310–316
    [Google Scholar]
  2. Aguilera A. 1987; Mutations suppressing the effects of a deletion of the phosphoglucose isomerase gene PGI1 in Saccharomyces cerevisiae . Current Genetics 11:429–434
    [Google Scholar]
  3. Aguilera A., Zimmermann F. K. 1986; Isolation and molecular analysis of the phosphoglucose isomerase structural gene of Saccharomyces cerevisiae . Molecular and General Genetics 202:83–89
    [Google Scholar]
  4. Cabib E. 1975; Molecular aspects of yeast morphogenesis. Annual Review of Microbiology 29:191–214
    [Google Scholar]
  5. Cabib E., Ulane R., Bowers B. 1974; A molecular model for morphogenesis: the primary septum of yeast. Current Topics in Cellular Regulation 8:1–32
    [Google Scholar]
  6. Ciriacy M., Breitenbach I. 1979; Physiological effects of seven different blocks in glycolysis in Saccharomyces cerevisiae . Journal of Bacteriology 139:152–160
    [Google Scholar]
  7. Clifton D., Weinstock S. B., Fraenkel D. 1978; Glycolysis mutants in Saccharomyces cerevisiae . Genetics 88:1–11
    [Google Scholar]
  8. Dickinson J. R., Dawes I. W. 1983; Ammonium ion repression of sporulation in Saccharomyces cerevisiae . Journal of General Microbiology 129:1883–1888
    [Google Scholar]
  9. Dickinson J. R., Williams A. S. 1986; A genetic and biochemical analysis of the role of gluconeogenesis in sporulation of Saccharomyces cerevisiae . Journal of General Microbiology 132:2605–2610
    [Google Scholar]
  10. Dickinson J. R., Williams A. S. 1987; The cdc30 mutation in Saccharomyces cerevisiae results in a temperature-sensitive isoen-zyme of phosphoglucose isomerase. Journal of General Microbiology 133:135–140
    [Google Scholar]
  11. Dickinson J. R., Smith M. E., Swanson T. R., Williams A. S., Wingfield J. M. 1988; The cdc30 mutation in Saccharomyces cerevisiae affects phosphoglucose isomerase, the cell cycle and sporulation. Journal of General Microbiology 134:2475–2480
    [Google Scholar]
  12. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  13. Green J. B. A., Wright A. P. H., Cheung W. Y., Lancashire W. E., Hartley B. S. 1988; The structure and regulation of phosphoglucose isomerase in Saccharomyces cerevisiae . Molecular and General Genetics 215:100–106
    [Google Scholar]
  14. Herrera L. S., Pascual C. 1978; Genetical and biochemical studies of glucosephosphate isomerase mutants in Saccharomyces cerevisiae . Journal of General Microbiology 108:305–310
    [Google Scholar]
  15. Kaback D. B., Oeller P. W., Steensma H. Y., Hirschman J., Ruezinsky D., Coleman K. G., Pringle J. R. 1984; Temperature-sensitive lethal mutations on yeast chromosome I appear to define only a small number of genes. Genetics 108:67–90
    [Google Scholar]
  16. Kawasaki G. H. 1979; Karyotypic instability and carbon source effects in cell cycle mutants of Saccharomyces cerevisiae. PhD thesis University of Washington; Seattle, USA:
    [Google Scholar]
  17. Kempe T. D., Nakagawa Y., Noltmann E. A. 1974a; Physical and chemical properties of yeast phosphoglucose isomerase isoen-zymes. Journal of Biological Chemistry 249:4617–4624
    [Google Scholar]
  18. Kempe T. D., Gee D. M., Hathaway G. M., Noltmann E. A. 1974b; Subunit and peptide compositions of yeast phosphoglucose isomerase isoenzymes. Journal of Biological Chemistry 249:4625–4633
    [Google Scholar]
  19. Maitra P. K. 1971; Glucose and fructose metabolism in a phosphoglucoseisomeraseless mutant of Saccharomyces cerevisiae . Journal of Bacteriology 107:759–769
    [Google Scholar]
  20. Maitra P. K., Lobo Z. 1977; Genetic studies with a phosphoglucose isomerase mutant of Saccharomyces cerevisiae . Molecular and General Genetics 156:55–60
    [Google Scholar]
  21. Mortimer R. K., Hawthorne D. C. 1975; Genetic mapping in yeast. Methods in Cell Biology 11:221–233
    [Google Scholar]
  22. Nakagawa Y., Noltmann E. A. 1967; Multiple forms of yeast phosphoglucose isomerase. I. Resolution of the crystalline enzyme into three isoenzymes. Journal of Biological Chemistry 242:4782–4788
    [Google Scholar]
  23. Pringle J. R. 1987; The gene number paradox and the complexity of the cell-division cycle and other cellular processes. In Modern Cell Biology 5299–306 Waymouth C. New York: Alan R. Liss;
    [Google Scholar]
  24. Sherman F. 1975; Use of micromanipulators in yeast studies. Methods in Cell Biology 11:189–199
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-4-765
Loading
/content/journal/micro/10.1099/00221287-137-4-765
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error