1887

Abstract

The gene coding for -galactosidase in was detected in a recombinant gene library constructed in phage . The gene was subcloned into plasmid vectors and shown to specify a novel protein of 80000. Characterization of -galactosidase from and from recombinant expressing indicated that the enzyme functions as a tetramer. The amino acid composition of the -galactosidase, deduced from nucleotide sequencing of , gave a predicted of 82022 and revealed regions of homology to -galactosidases encoded by the Raf plasmids and by . Inactivation of the gene in resulted in loss of all -galactosidase activity and abolished the ability to ferment melibiose; -glucosidase activity was also lost, due to an indirect effect on the gene.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-4-757
1991-04-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/4/mic-137-4-757.html?itemId=/content/journal/micro/10.1099/00221287-137-4-757&mimeType=html&fmt=ahah

References

  1. Aduse-Opoku J., Gilpin M. L., Russell R. R. B. 1990; Genetic and antigenic comparison of Streptococcus mutans fructosyltransferase and glucan-binding protein. FEMS Microbiology Letters 59:279–282
    [Google Scholar]
  2. Aslanidis C., Schmid K., Schmitt R. 1989; Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli . Journal of Bacteriology 171:6753–6763
    [Google Scholar]
  3. Bailey R. W. 1963; The intracellular α-galactosidase of a rumen strain of Streptococcus bovis . Biochemical Journal 86:509–514
    [Google Scholar]
  4. Barletta R. G., Curtiss R. 1989; Impairment of melibiose utilization in Streptococcus mutans serotype c gtfA mutants. Infection and Immunity 57:992–995
    [Google Scholar]
  5. Barletta R. G., Michalek S. M., Curtiss R. 1988; Analysis of the virulence of Streptococcus mutans serotype c gtfA mutants in the rat model system. Infection and Immunity 56:322–330
    [Google Scholar]
  6. Burstein C., Kepes A. 1971; The αgalactosidase from Escherichia coli K12. Biochimica et Biophysica Ada 230:52–63
    [Google Scholar]
  7. Ferretti J. J., Gilmore K. S., Courvalin P. 1986; Nucleotide sequence analysis of the gene specifying the bifunctional 6′-aminoglycoside acetyltransferase 2″-aminoglycoside phosphotrans-ferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activities. Journal of Bacteriology 167:631–638
    [Google Scholar]
  8. Ferretti J. J., Huang T. T., Russell R. R. B. 1988; Sequence analysis of the glucosyltransferase A (gtfA) gene from Streptococcus mutans Ingbritt. Infection and Immunity 56:1585–1588
    [Google Scholar]
  9. Ganter C., Bock A., Buckel P., Mattes R. 1988; Production of thermostable, recombinant αgalactosidase suitable for raffinose elimination from sugar beet syrup. Journal of Biotechnology 8:301–310
    [Google Scholar]
  10. Gilpin M. L., Russell R. R. B., Morrissey P. 1985; Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12. Infection and Immunity 49:414–416
    [Google Scholar]
  11. Hamada S., Slade H. D. 1980; Biology, immunology, and cariogenicity of Streptococcus mutans . Microbiological Reviews 44:331–384
    [Google Scholar]
  12. Hayakawa M., Aoki H., Kuramitsu H. K. 1986; Isolation and characterization of the sucrose 6-phosphate hydrolase gene from Streptococcus mutans . Infection and Immunity 53:582–586
    [Google Scholar]
  13. Von Heijne G., Abrahmsen L. 1989; Species-specific variation in signal peptide design. FEBS Letters 244:439–446
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  15. Li Y.-T., Li S.-C. C., Shetlar M. R. 1963; αGalactosidase from Diplococcus pneumoniae . Archives of Biochemistry and Biophysics 103:436–442
    [Google Scholar]
  16. Liljestrom P. L., Liljestrom P. 1987; Nucleotide sequence of the melA gene, coding for αgalactosidase in Escherichia coli K-12. Nucleic Acids Research 15:2213–2220
    [Google Scholar]
  17. Loesche W. J. 1986; Role of Streptococcus mutans in human dental decay. Microbiological Reviews 50:355–380
    [Google Scholar]
  18. Lunsford R. D., Macrina F. L. 1986; Molecular cloning and characterization of scrB the structural gene for the Streptococcus mutans phosphoenolpyruvate-dependent sucrose phosphotransferase system sucrose-6-phosphate hydrolase. Journal of Bacteriology 166:426–434
    [Google Scholar]
  19. Macrina F. L., Evans P. R., Tobian J. A., Hartley D. L., Clewell D. B., Jones K. R. 1983; Novel shuttle plasmid vehicles for Escherichia coli-Streptococcus mutans . Gene 25:145–150
    [Google Scholar]
  20. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Nagao Y., Nakada T., Imoto M., Shimamoto T., Sakai S., Tsuda M., Tsuchiya T. 1988; Purification and analysis of the structure of αgalactosidase from Escherichia coli . Biochemical and Biophysical Research Communications 151:236–241
    [Google Scholar]
  22. Perry D., Kuramitsu H. K. 1981; Genetic transformation of Streptococcus mutans . Infection and Immunity 41:722–727
    [Google Scholar]
  23. Perry D., Nilsen L. J., Kuramitsu H. K. 1985; Mapping of a cloned glucosyltransferase gene in Streptococcus mutans . Infection and Immunity 50:130–135
    [Google Scholar]
  24. Russell R. R. B., Ferretti J. J. 1990; Nucleotide sequence of the dextran glucosidase (dexB) gene of Streptococcus mutans . Journal of General Microbiology 136:803–810
    [Google Scholar]
  25. Russell R. R. B., Morrissey P., Dougan G. 1985; Cloning of sucrase genes from Streptococcus mutans in bacteriophage lambda. FEMS Microbiology Letters 30:37–41
    [Google Scholar]
  26. Russell R. R. B., Mukasa H., Shimamura A., Ferretti J. J. 1988; Streptococcus mutans gtfA gene specifies sucrose phosphorylase. Infection and Immunity 56:2763–2765
    [Google Scholar]
  27. Russell R. R. B., Aduse-Opuku J., Tao L., Ferretti J. J. 1991; A binding protein-dependent transport system in Streptococcus mutans . In Genetics and Molecular Biology of Streptococci, Lactococci, and Enterococci Dunny G., Cleary P., McKay L. Washington, DC: American Society for Microbiology (in the Press);
    [Google Scholar]
  28. Schmid K., Schmitt R. 1976; Raffinose metabolism in Escherichia coli K-12. Purification and properties of a new αgalactosidase specified by a transmissible plasmid. European Journal of Biochemistry 67:95–104
    [Google Scholar]
  29. Schmitt R. 1968; Analysis of melibiose mutants deficient in αgalactosidase and thiomethylgalactoside permease II in Escherichia coli K-12. Journal of Bacteriology 96:462–471
    [Google Scholar]
  30. Schmitt R., Rotman B. 1966; αGalactosidase activity in cell-free extracts of Escherichia coli. Biochemical and Biophysical Research Communications 22:473–479
    [Google Scholar]
  31. Schmitt R., Mattes R., Schmid K., Altenbucher J. 1979; Raf plasmids in strains of Escherichia coli and their possible role in enteropathogenicity. In Plasmids of Medical, Environmental and Commercial Importance199–210 Timmis K. N., Puhler A. Amsterdam: Elsevier;
    [Google Scholar]
  32. Shklair I. L., Keene H. J. 1974; A biochemical scheme for the separation of the five varieties of Streptococcus mutans . Archives of Oral Biology 9:1079–1081
    [Google Scholar]
  33. Terleckyj B., Willett P. N., Shockman G. D. 1975; Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infection and Immunity 11:649–655
    [Google Scholar]
  34. Tubb R. S., Liljestrom P. L. 1986; A colony-colour method which differentiates αgalactosidase-positive strains of yeast. Journal of the Institute of Brewing 92:588–590
    [Google Scholar]
  35. Wilbur W. J., Lipman D. J. 1982; Rapid similarity searches of nucleic acid and protein data banks. Proceedings of the National Academy of Sciences of the United States of America 80726–730
    [Google Scholar]
  36. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of M13 mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-4-757
Loading
/content/journal/micro/10.1099/00221287-137-4-757
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error