1887

Abstract

Summary: The gene coding for α-galactosidase in was detected in a recombinant gene library constructed in phage λ. The gene was subcloned into plasmid vectors and shown to specify a novel protein of 80000. Characterization of α-galactosidase from and from recombinant expressing indicated that the enzyme functions as a tetramer. The amino acid composition of the α-galactosidase, deduced from nucleotide sequencing of , gave a predicted of 82022 and revealed regions of homology to α-galactosidases encoded by the Raf plasmids and by . Inactivation of the gene in resulted in loss of all α-galactosidase activity and abolished the ability to ferment melibiose; α-glucosidase activity was also lost, due to an indirect effect on the gene.

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/content/journal/micro/10.1099/00221287-137-4-757
1991-04-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-4-757
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