1887

Abstract

Starved cultures of the soil amoeba suspended in phosphate-buffered saline exhibit stimulated O uptake in response to phagocytosis of either heat-killed yeast or latex beads. This phagocytosis-dependent (cyanide-insensitive) oxidative activity was observed when cells were isolated from either cyanide-stimulated or -inhibited cultures, and was therefore independent of the relative activities of the alternative mitochondrial oxidases known to exist in this organism. The extra O consumed during phagocytosis was stoichiometrically converted into O as detected by the rate of superoxide dismutase-inhibitable cytochrome reduction. Phagolysosomal membranes isolated after uptake of latex beads were enriched in a -type cytochrome which was spectrally similar to that present in immune phagocytes. Thus, the biochemical events during phagocytosis by either or immune phagocytes appear similar, suggesting that the ‘respiratory burst’ enzyme(s) responsible for O generation in these two cell types is structurally related.

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1991-03-01
2024-03-28
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References

  1. Allen R. C. 1981; Lucigenin chemiluminescence : a new approach to the study of polymorphonuclear leukocyte redox activity. In Bioluminescence and Chemiluminescence63–73 De Luca M. A. New York: Academic Press;
    [Google Scholar]
  2. Allen R. C., Loose L. D. 1976; Phagocytic activation of a luminol-dependent chemiluminescence in rabbit alveolar and peritoneal macrophages. Biochimica et Biophysica Acta 96:245–252
    [Google Scholar]
  3. Babior B. M. 1984; Oxidants from phagocytes : agents of defense and destruction. Blood 64:959–966
    [Google Scholar]
  4. Babior M. M., Kipnes R. S., Curnutte J. T. 1973; Biological defense mechanisms : the production by leukocytes of Superoxide, a potential bacteriocidal agent. Journal of Clinical Investigation 52:741–744
    [Google Scholar]
  5. Brooks S. E., Schneider D. L. 1985; Oxidative metabolism associated with phagocytosis in Acanthamoeba castellanii. Journal of Protozoology 32:330–333
    [Google Scholar]
  6. Curnutte J. T. 1988; Phagocyte Defects I and II. Hematology/Oncology Clinics of North America; Philadelphia, USA: Saunders:
    [Google Scholar]
  7. Davies B., Edwards S. W. 1989; Inhibition of myeloperoxidase by salicylhydroxamic acid. Biochemical Journal 258:801–806
    [Google Scholar]
  8. Edwards S. W. 1987; Luminol- and lucigenin-dependent chemiluminescence of neutrophils : role of degranulation. Journal of Clinical and Laboratory Immunology 22:35–39
    [Google Scholar]
  9. Edwards S. W., Doulah F. A. 1982; Elevation of AMP levels during phagocytosis in Acanthamoeba castellanii. Journal of General Microbiology 128:2919–2925
    [Google Scholar]
  10. Edwards S. W., Lloyd D. 1977; Cyanide-insensitive respiration in Acanthamoeba castellanii. Changes in sensitivity of whole cell respiration during exponential growth. Journal of General Microbiology 103:207–213
    [Google Scholar]
  11. Edwards S. W., Say J. E., Hart C. A. 1987; Oxygen-dependent killing of Staphylococcus aureus by human neutrophils. Journal of General Microbiology 133:3591–3597
    [Google Scholar]
  12. Hallett M. B., Edwards S. W., Campbell A. K. 1987; Control of oxygen radical production by polymorphonuclear leucocytes monitored by luminol-dependent chemiluminescence: the role of intracellular calcium, oxygen concentration and redox components. In Cellular Chemiluminescence173–192 Von Dyke E. Boca Raton, Florida: CRC Press;
    [Google Scholar]
  13. Holmes B., Page A. R., Good R. A. 1967; Studies of the metabolic activity of leukocytes from patients with a genetic abnormality of phagocytic function. Journal of Clinical Investigation 46:1422–1432
    [Google Scholar]
  14. Halliwell B., Gutteridge J. M. C. 1985; Free Radicals in Biology and Medicine. Oxford: Clarendon Press;
    [Google Scholar]
  15. Klebanoff S. J., Clark R. A. 1978; The Neutrophil: Function and Clinical Disorders. Amsterdam: North-Holland;
    [Google Scholar]
  16. Korn E. D. 1974; The isolation of the amoeba plasma membrane and the use of latex beads for the isolation of phagocytic vacuole (phagosome) membranes from amoebae including the culture techniques for amoebae. Methods in Enzymology 31:686–698
    [Google Scholar]
  17. Lloyd D., Edwards S. W., Kristensen B., Degn H. 1979; The effects of inhibitors on the oxygen kinetics of terminal oxidases of Acanthamoeba castellami. Biochemical Journal 182:11–15
    [Google Scholar]
  18. Ohnishi T., Kroger A., Heldt H. W., Pfaff E., Klingenberg M. 1967; The response of the respiratory chain and adenine nucleotide system to oxidative phosphorylation in yeast mitochon-dria. European Journal of Biochemistry 1:301–311
    [Google Scholar]
  19. Pember S. O., Shapira R., Kinkade J. M. Jr 1983; Multiple forms of myeloperoxidase from human neutrophilic granulocytes: evidence for differences in compartmentalisation, enzymatic activity and sub-unit structure. Archives of Biochemistry and Biophysics 221:391–403
    [Google Scholar]
  20. Rossi F. 1986; The forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function. Biochimica et Biophysica Acta 853:65–89
    [Google Scholar]
  21. Royer-Pokora B., Kunkel L. M., Monaco A. P., Goff S. C., Newburger P. E., Baehner R. L., Cole F. S., Curnutte J. T., Orkin S. H. 1986; Cloning of the gene for an inherited human disorder - chronic granulomatous disease - on the basis of its chromosomal location. Nature, London 322:32–38
    [Google Scholar]
  22. Segal A. W., Jones O. T. G. 1978; Novel cytochrome b system in phagocytic vesicles of human granulocytes. Nature London: 276515–517
    [Google Scholar]
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