1887

Abstract

Control of methionine biosynthesis in K12 was reinvestigated by using methionine-analogue-resistant mutants. Norleucine (NL) and a-methylmethionine (MM) were found to inhibit methionine biosynthesis directly whereas ethionine (Et) competitively inhibited methionine utilization. Adenosylation of Et to generate -adenosylethionine (AdoEt) by cell-free enzyme from K12 was demonstrated. Tolerance of increasing concentrations of NL by K12 mutants is expressed serially as phenotypes NL, NLEt, NLMM and finally NLEtMM. All spontaneous NL mutants had a mutation, whereas NTG-induced mutants had mutations in both the and genes. The kinetics of methionine adenosylation by the K12 cell-free enzyme were found to be similar to those reported for the yeast enzyme, showing the typical lag phase at low methionine concentration and disappearance of this phase when AdoMet was included in the incubation mixture. NL extended the lag phase, and lowered the rate of subsequent methionine adenosylation, but did not affect the shortening of the lag phase of adenosylation by AdoMet.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-3-685
1991-03-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/3/mic-137-3-685.html?itemId=/content/journal/micro/10.1099/00221287-137-3-685&mimeType=html&fmt=ahah

References

  1. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N’-nitro-N-nitroso guani-dine in Escherichia coli K12. Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  2. Bachmann B. J. 1983; Linkage map of Escherichia coli K12 (edition 7). Microbiological Reviews 47:180–230
    [Google Scholar]
  3. Chou T. C., Talalay P. 1972; The mechanism of S-adenosylmeth-ionine synthesis by purified preparations of baker’s yeast. Biochemistry 11:1065–1073
    [Google Scholar]
  4. Cox R., Smith R. C. 1969; Inhibition of S-adenosyl methionine formation by analogues of methionine. Archives of Biochemistry and Biophysics 129:615–619
    [Google Scholar]
  5. Davis B. D., Mingioli E. S. 1950; Mutants of Escherichia coli requiring methionine or vitamin B2 . Journal of Bacteriology 60:17–28
    [Google Scholar]
  6. Dev I. K., Harvey R. J. 1984; Role of methionine in the regulation of the synthesis of serine hydroxymethyl transferase in Escherichia coli . Journal of Biological Chemistry 259:8402–8406
    [Google Scholar]
  7. Glazer R. I., Peale A. L. 1978; Measurement of S-adenosyl-L-methionine level by SP-Sephadex chromatography. Analytical Biochemistry 91:516–520
    [Google Scholar]
  8. Greene R. C, Su C. H., Holloway C. T. 1970; S-adenosyl methionine synthetase deficient mutants of Escherichia coli K12 with impaired control of methionine biosynthesis. Biochemical and Biophysical Research Communications 38:1120–1126
    [Google Scholar]
  9. Greene R. C, Hunter J. S.V., Coch E. H. 1973; Properties of metK mutants of Escherichia coli K12. Journal of Bacteriology 115:57–67
    [Google Scholar]
  10. Greene R. C, Radovich C. 1975; Role of methionine in the regulation of serine hydroxymethyl transferase in Escherichia coli . Journal of Bacteriology 124:269–278
    [Google Scholar]
  11. Hobson A. C., Smith D. A. 1973; S-adenosyl methionine synthetase in methionine regulatory mutants of Salmonella typhimurium. Molecular and General Genetics 126:7–18
    [Google Scholar]
  12. Kung H. F., Spears C, Greene R. C., Weissbach H. 1972; Regulation of terminal reactions in methionine biosynthesis by vitamin B12 or methionine. Archives of Biochemistry and Biophysics 150:23–31
    [Google Scholar]
  13. Lee L. H., Ravel J. M., Shive W. 1966; Multimetabolite control of a biosynthetic pathway by sequential metabolites. Journal of Biological Chemistry 241:5479–5480
    [Google Scholar]
  14. Markham G. D., Hafner E. W., Tabor C. W., Tabor H. 1980; S-Adenosylemethionine synthetase from Escherichia coli . Journal of Biological Chemistry 255:9082–9092
    [Google Scholar]
  15. Martin W. R., Moo-Penn W. F. 1963; Studies on the mechanism of Escherichia coli resistance to ethionine. Proceedings of the Society for Experimental Biology and Medicine 114:666–669
    [Google Scholar]
  16. Meedel T. H., Pizer L. I. 1974; Regulation of one carbon biosynthesis and utilization in Escherichia coli . Journal of Bacteriology 118:905–910
    [Google Scholar]
  17. Miller J. H. 1972; Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Park L. W. 1958; S-Adenosylethionine and ethionine inhibition. Journal of Biological Chemistry 232:169–176
    [Google Scholar]
  19. Peterkofsky A. 1965; Alteration of the macromolecular structure of nucleic acid by transmethylation. Transmethylation and Methionine Biosynthesis115–137 Shapiro S. K, Schlenk F. Chicago, Illinois: University of Chicago Press;
    [Google Scholar]
  20. Schlesinger S. 1967; Inhibition of growth of Escherichia coli and of homoserine O-transsuccinylase by α-methylmethionine. Journal of Bacteriology 94:327–332
    [Google Scholar]
  21. Shoeman R., Redfield B., Coleman T., Greene R. C, Smith A. A., Brot N., Weissbach H. 1985a; Regulation of methionine synthesis in Escherichia coli: effect of metJ gene product and S-adenosylmethionine on the expression of the metF gene. Proceedings of the National Academy of Sciences of the United States of America 82:3601–3605
    [Google Scholar]
  22. Shoeman R., Coleman T., Redfield B., Greene R. C, Smith A. A., Saint-Girons I., Brot N., Weissbach H. 1985b; Regulation of methionine synthesis in Escherichia coli: effect of met J gene product and S-adenosylmethionine on the in vitro expression of metB, metL and metJ genes. Biochemical and Biophysical Research Communications 133:731–739
    [Google Scholar]
  23. Smith D. A. 1971; S-Amino acid metabolism and its regulation in Escherichia coli and Salmonella typhimurium. Advances in Genetics 16:142–161
    [Google Scholar]
  24. Su C. H., Greene R. C., Holloway C. T. 1970; Regulation of S-adenosyl methionine synthetase in Escherichia coli K12. Bacteriological Proceedings 70:136
    [Google Scholar]
  25. Tabor H., Tabor C. W. 1971; Enzymatic synthesis of radioactive S-adenosyl-L-methionine. Methods in Enzymology 17B:393–397
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-3-685
Loading
/content/journal/micro/10.1099/00221287-137-3-685
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error