1887

Abstract

In , the expression of the phenylalanine biosynthetic operon is regulated by an attenuation mechanism. In the presence of excess phenylalanine, gene expression was decreased to 10% of the fully deattenuated level. To understand the factors that determine the basal level of expression, we examined the role of ribosome release from the leader peptide stop codon UGA. The transcriptional readthrough from the attenuator decreased by over 2-fold in the presence of the defective release factor 2. However, a release factor 1 (UAG and UAA specific) mutation did not influence the basal level of expression. These results support the proposal that the release of translating ribosomes from the leader peptide stop codon in stem 2 of the attenuator plays a crucial role in determining the basal level of expression of this gene.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-3-679
1991-03-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/3/mic-137-3-679.html?itemId=/content/journal/micro/10.1099/00221287-137-3-679&mimeType=html&fmt=ahah

References

  1. Adhya S., Gottesman M. 1978; Control of transcription termination. Annual Review of Biochemistry 47:967–996
    [Google Scholar]
  2. Caskey C. T. 1980; Peptide chain termination. Trends in Biochemical Sciences 5:234–237
    [Google Scholar]
  3. Craigen W. J., Caskey C. T. 1986; Expression of peptide chain release factor 2 requires high efficiency frameshift. Nature London: 322273–275
    [Google Scholar]
  4. Craigen W. J., Cook R. J., Tate W. P., Caskey C. T. 1985; Bacterial peptide chain release factors : conserved primary structure and possible frameshift regulation of release factor 2. Proceedings of the National Academy of Sciences of the United States of America 82:3616–3620
    [Google Scholar]
  5. Gavini N. 1988; Investigations on the regulation of the Escherichia coli gene pheA . PhD dissertation, University of Melbourne Victoria Australia:
    [Google Scholar]
  6. Gold L., Storm G. 1987; Translational initiation. In Escherichia coli and Salmonella typhimurium : Cellular and Molecular Biology 21302–1307 Neidhardt F., Ingraham J. L., Brooks-Low K., Magasanik B., Schaechter M., and Umbarger H. E. American Society for Microbiology; Washington DC:
    [Google Scholar]
  7. Gowrishankar J., Pittard J. 1982a; Construction from Mu dl (lac Apr) lysogens of lambda bacteriophage bearing promoter-lac fusions : isolation of λ ppheA-lac . Journal of Bacteriology 150:1122–1129
    [Google Scholar]
  8. Gowrishankar J., Pittard J. 1982b; Regulation of phenylalanine biosynthesis in Escherichia coli K-12 : control of transcription of the phe A operon. Journal of Bacteriology 150:1130–1137
    [Google Scholar]
  9. Grunberg-Manago M. 1987; Regulation of the expression of aminoacyl-tRNA synthetases and translation factors. In Escherichia coli and Salmonella typhimurium : Cellular and Molecular Biology 21386–1409 Neidhardt F., Ingraham J. L., Brooks-Low K., Magasanik B., Schaechter M., and Umbarger H. E. American Society for Microbiology; Washington DC:
    [Google Scholar]
  10. Hershey J. W. B. 1987; Protein synthesis. In Escnerichia coli and Salmonella typhimurium: Cellular and Molecular Biology 1613–647 Neidhardt F., Ingraham J. L., Brooks-Low K., Magasanik B., Schaechter M., Umbarger H. E. American Society for Microbiology; Washington DC:
    [Google Scholar]
  11. Hudson G. S., Davidson B. E. 1984; Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K-12. Journal of Molecular Biology 180:1023–1051
    [Google Scholar]
  12. Im S. W. K., Pittard J. 1971; Phenylalanine biosynthesis in Escherichia coli K-12 : mutants derepressed for chorismate mutase P-prephenate dehydratase. Journal of Bacteriology 106:784–790
    [Google Scholar]
  13. Kawakami K., Inada T., Nakamura Y. 1988; Conditionally lethal and recessive UGA-suppressor mutations in the prfB gene encoding peptide chain release. Journal of Bacteriology 170:5378–5381
    [Google Scholar]
  14. Kolter R., Yanofsky C. 1982; Attenuation in amino acid biosynthetic opérons. Annual Review of Genetics 16:113–134
    [Google Scholar]
  15. Landick R., Yanofsky C. 1987; Transcription attenuation. In Escherichia coli and Salmonella typhimurium : Cellular and Molecular Biology 21453–1472 Neidhardt F., Ingraham J. L., Brooks-Low K., Magasanik B., Schaechter M., Umbarger H. E. American Society for Microbiology; Washington DC:
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: A Laboratory Manual . Cold Spring Harbor Laboratory Cold Spring Harbor, N.Y:
    [Google Scholar]
  17. Miller J. H. 1972; Experiments in Molecular Genetics . Cold Spring Harbor Laboratory Cold Spring Harbor, N.Y:
    [Google Scholar]
  18. Miller J. H., Albertini A. M. 1983; Effects of surrounding sequence on the suppression of nonsense codons. Journal of Molecular Biology 164:59–71
    [Google Scholar]
  19. Pittard A. J. 1987; Biosynthesis of the aromatic amino acids. In Escherichia coli and Salmonella typhimurium : Cellular and Molecular Biology 1368–394 Neidhardt F., Ingraham J. L., Brooks-Low K., Magasanik B., Schaechter M., Umbarger H. E. American Society for Microbiology; Washington DC:
    [Google Scholar]
  20. Roesser J. R,., Yanofsky C. 1988; Ribosome release modulates basal level expression of the trp operon of Escherichia coli . Journal of Biological Chemistry. 263:14251–14255
    [Google Scholar]
  21. Roesser J. R,., Nakamura Y., Yanofsky C. 1989; Regulation of basal level expression of the tryptophan operon of Escherichia coli . Journal of Biological Chemistry 264:12284–12288
    [Google Scholar]
  22. Ryden S. M., Isaksson L. A. 1984; A temperature-sensitive mutant of Escherichia coli that shows enhanced misreading of UAG/A and increased efficiency for some tRNA non-sense suppressors. Molecular and General Genetics 193:38–45
    [Google Scholar]
  23. Ryden M., Murphy J., Martin R., Isaksson L., Gallant J. 1986; Mapping and complementation studies of the gene for release factor 1. Journal of Bacteriology 168:1066–1069
    [Google Scholar]
  24. Simons R. W., Houman F., Kleckner N. 1987; Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53:85–96
    [Google Scholar]
  25. Soll L. 1974; Mutational alterations of tryptophan-specific transfer RNA that generates translational suppressors of the UAA, UAG, and UGA nonsense codons. Journal of Molecular Biology 86:233–243
    [Google Scholar]
  26. Tinoco Jr. I., Borer P. N., Dengler B., Levine M. D., Uhlenbeck O. C., Crothers D. M., Gralla J. 1973; Improved estimation of secondary structure in ribonucleic acids. Nature New Biologist 246:40–41
    [Google Scholar]
  27. Yager T. H., Von Hippel P. H. 1987; Transcript elongation and termination in Escherichia coli . In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 21241–1275 Neidhardt F., Ingraham J. L., Brooks-Low K., Magasanik B., Schaechter M., Umbarger H. E. American Society for Microbiology; Washington DC:
    [Google Scholar]
  28. Yanofsky C. 1981; Attenuation in the control of expression of bacterial operons. Nature London: 289751–758
    [Google Scholar]
  29. Yanofsky C. 1988; Transcription attenuation. Journal of Biological Chemistry 263:609–612
    [Google Scholar]
  30. Yanofsky C., Kelly R. L., Horn V. 1984; Repression is relieved before attenuation in the trp operon of Escherichia coli as tryptophan starvation becomes increasingly severe. Journal of Bacteriology 158:1018–1024
    [Google Scholar]
  31. Zurawski G., Brown K., Killingly D., Yanofsky C. 1978; Nucleotide sequence of the leader region of phenylalanine operon of Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 75:4271–4275
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-3-679
Loading
/content/journal/micro/10.1099/00221287-137-3-679
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error