1887

Abstract

The cell wall of was found to have a three-layered structure when thin sections of whole cells or isolated cell walls were stained by various procedures and examined by electron microscopy. The main feature of the composition of isolated cell walls was a high protein content (about 48% of the cell wall dry weight) with a predominant 52 kDa protein. Peptidoglycan and what was considered as teichoic acids accounted for 36% and 14%, respectively, of the cell wall dry weight. The 52 kDa protein was apparently unglycosylated, located in the outer layer and noncovalently bound to the inner layers. Peptidoglycan was mostly assigned to the innermost layer. The conditions leading to autolysis of whole cells or isolated cell walls were determined, as well as the specificity of the autolysin involved in cell wall degradation.

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1991-03-01
2021-05-10
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References

  1. Accolas J. P., Hemme D., Desmazeaud J. M., Vassal L., Bouillane C., Veaux M. 1980; Les levains lactiques thermo-philes: propriétés et comportement en technologie laitière. Lait 60:487–517
    [Google Scholar]
  2. Arnold B. H., Hall A. N. 1955; Effect of penicillin and of streptomycin on certain species of Lactobacillus and Acetobacter. Journal of the Institute of Brewing 61:44–45
    [Google Scholar]
  3. Baddiley J., Davison J. L. 1961; The occurrence and location of teichoic acids in lactobacilli. Journal of General Microbiology 24:295–299
    [Google Scholar]
  4. Bie R., Sjostrom G. 1975a; Autolytic properties of some lactic acid bacteria used in cheese production. Part I. Material and methods. Milchwissenschaft 30:653–657
    [Google Scholar]
  5. Bie R., Sjostrom G. 1975b; Autolytic properties of some lactic acid bacteria used in cheese production. Part II. Experiments with fluid substrates and cheese. Milchwissenschaft 30:739–747
    [Google Scholar]
  6. Chassy B. M. 1987; Prospects for the genetic manipulation of lactobacilli. FEMS Microbiology Reviews 46:297–312
    [Google Scholar]
  7. Coyette J., Ghuysen J. M. 1970a; Structure of the walls of Lactobacillus acidophilus strain 63 AM Gasser. Biochemistry 9:2935–2943
    [Google Scholar]
  8. Coyette J., Ghuysen J. M. 1970b; Wall autolysinof Lactobacillus acidophilus strain 63 AM Gasser. Biochemistry, 9:2952–2955
    [Google Scholar]
  9. Coyette J., Shockman G. D. 1973; Some properties of the autolytic 7V-acetylmuramidase of Lactobacillus acidophilus . Journal of Bacteriology, 114:34–41
    [Google Scholar]
  10. De Man J. C., Rogosa M., Sharpe E. 1960; A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology 23:130–135
    [Google Scholar]
  11. De Servio A. J. 1969; Alterations of the phospholipid composition of E. coli B during growth at different temperatures. Journal of Bacteriology 100:1342–1349
    [Google Scholar]
  12. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–358
    [Google Scholar]
  13. Ezzat N., El Soda M., Desmazeaud M. J., Ismail A. 1986; Peptide hydrolases from Thermobacterium group of lactobacilli. III. Characterization of intracellular peptidase. Lait 66:445–451
    [Google Scholar]
  14. Frehel C., Ryter A. 1982; Electron microscopic cytochemical study of cell wall polysaccharides in Bacillus subtilis and two strains of Bacillus megaterium . Journal of Ultrastructure Research 81:66–77
    [Google Scholar]
  15. Frehel C., Robbe P., Tinelli R., Ryter A. 1982; Relationship between biochemical results obtained on Bacillus megaterium and Bacillus subtilis cell-wall polysaccharides. Journal of Ultrastructure Research 81:77–80
    [Google Scholar]
  16. French C. L., Milner H. W. 1955; Disintegration of bacteria and small particles by high pressure extrusion. Methods in Enzymology 1:64–67
    [Google Scholar]
  17. Ghuysen J. M., Tipper D. J., Strominger J. L. 1966; Enzymes that degrade bacterial cell walls. Methods in Enzymology, 8:885–899
    [Google Scholar]
  18. Hammes W., Schleifer K. H., Kandler O. 1973; Mode of action of glycine on the biosynthesis of peptidoglycan. Journal of Bacteriology 116:1029–1053
    [Google Scholar]
  19. Hara S., Matsushima Y. 1966; Studies on amino-hexoses. XII. Crystalline muramicitol and its use in a study of the lysozyme action on the cell wall of Micrococcus lysodeikticus . Bulletin of the Society of Chemistry of Japan 39:1826
    [Google Scholar]
  20. Higgins M. L., Coyette J., Shockman G. D. 1973; Sites of cellular autolysis in Lactobacillus acidophilus . Journal of Bacteriology 116:1054–1065
    [Google Scholar]
  21. Hungerer K. D., Pleck J., Tipper D. J. 1969; Structure of the cell wall peptidoglycan of Lactobacillus casei R094. Biochemistry 8:3567–3577
    [Google Scholar]
  22. Ikawa M., Snell E. E. 1960; Cell wall composition of lactic acid bacteria. Journal of Biological Chemistry 235:1376–1382
    [Google Scholar]
  23. Kakefuda T., Holden J. J., Utech N. M. 1967; Ultrastructure of the membrane system in Lactobacillus plantarum . Journal of Bacteriology 93:472–482
    [Google Scholar]
  24. Kapitany R. A., Zebrowski E. J. 1973; A high resolution PAS stain for polyacrylamide gel electrophoresis. Analytical Biochemistry 56:361–369
    [Google Scholar]
  25. Kawata T., Masuda K., Yoshino K., Fujimoto M. 1985; Regular array in the cell wall of Lactobacillus fermenti as revealed by freeze-etching and negative staining. Japanese Journal of Microbiolo-gy 18:469–476
    [Google Scholar]
  26. Knox K. W., Wicken A. J. 1971; Serological properties of the wall and membrane teichoic acids from Lactobacillus helveticus NCIB 8025. Journal of General Microbiology 63:237–248
    [Google Scholar]
  27. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  28. Leduc M., Frehel C., Siegel E., van Heijenoort J. 1989; Multilayered distribution of peptidoglycan in the periplasmic space of Escherichia coli . Journal of General Microbiology 135:1–12
    [Google Scholar]
  29. Lortal S., Boyaval P., van Heijenoort J. 1989; Influence de plusieurs facteurs sur l’autolyse de Lactobacillus helveticus CNRZ 414. Lait 69:223–231
    [Google Scholar]
  30. Masuda K., Kawata T. 1979; Ultrastructure and partial characterization of a regular array (RA) in the cell wall of Lactobacillus brevis . Microbiology and Immunology 23:941–953
    [Google Scholar]
  31. Masuda K., Kawata T. 1983; Distribution and chemical characterization of regular arrays in the cell wall of strains of the genus Lactobacillus . FEMS Microbiology Letters 20:145–150
    [Google Scholar]
  32. Neujahr H. Y., Logardt I. M. 1973; Autolytic enzyme system from L. fermenti . Biochemistry 12:2578–2583
    [Google Scholar]
  33. Ohmiya K., & Sato Y. 1970; Studies on the proteolytic action of dairy lactic acid bacteria. X. Autolysis of lactic acid bacteria cells in aseptic rennet curd. Agricultural and Biochemical Chemistry 34:457–463
    [Google Scholar]
  34. Rambourg A. 1969; Localisation ultrastructurale et nature du matériel coloré au niveau de la surface cellulaire par le mélange chromique-phosphotungstique. Journal de Microscopie 8:325–342
    [Google Scholar]
  35. Rogers H. J., Perkins H. R., Ward J. B. 1980 Microbial Cell Walls and Membranes454–455 New York: Chapman & Hall;
    [Google Scholar]
  36. Rousseau M., Hermier J. 1975; Localisation en microscopie électronique des polysaccharides de la paroi chez les bactéries en sporulation. Journal de Microscopie et de Biologie Cellulaire 23:237–248
    [Google Scholar]
  37. Sandine W. E. 1987; Looking backward and forward at the practical application of genetic researches on lactic acid bacteria. FEMS Microbiology Letters 46:205–220
    [Google Scholar]
  38. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriological Reviews 36:407–477
    [Google Scholar]
  39. Sleytr V. B., Messner P. 1988; Crystalline surface layer in procaryotes. Journal of Bacteriology 170:2891–2897
    [Google Scholar]
  40. Spackman D. H., Stein W. H., Moore S. 1958; Automatic recording apparatus for use in the chromatography of amino-acids. Analytical Chemistry 30:1190–1206
    [Google Scholar]
  41. Swallow D. L., Abraham E. P. 1958; Formation of ε- aminosuccinyl-lysine from ε-aspartyl-lysine from bacitracin A, and from the cell walls of lactobacilli. Biochemical Journal 70:364–373
    [Google Scholar]
  42. Thiery J. M. 1967; Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. Journal of Microscopy 6:987–1018
    [Google Scholar]
  43. Thomas J. K., Collins M. A. 1988; Evidence for the conjugal transfer of the broad host range plasmid pIP501 into strains of Lactobacillus helveticus . Journal of Applied Bacteriology 65:309
    [Google Scholar]
  44. Thompson J. S., Shockman G. D. 1968; Modification of the Park and Johnson reducing sugars determination suitable for the assay of insoluble materials: its application to bacterial cell walls. Analytical Biochemistry 22:260–268
    [Google Scholar]
  45. Torre G. D., Davanzo A. 1954; Saggi sperimentali sul grado di sensibilità di un ceppo di L. arabinosus, L. helveticus e L. leichmanii, verso gli antibiotici. Latte 28:647–648
    [Google Scholar]
  46. Turner K. W., Morris H. A., Martley F. G. 1983; Swiss type cheese. II. The role of thermophilic lactobacilli in sugar fermentation. New Zealand Journal of Dairy Science Technology 18:117–123
    [Google Scholar]
  47. Wallinder I. B., Neujahr H. Y. 1971; Cell wall and peptidoglycan from Lactobacillus fermenti . Journal of Bacteriology 105:918–926
    [Google Scholar]
  48. Wilkowske H. H., Krienke W. A. 1951; Influence of penicillin the lactic acid production of certain lactobacilli. Journal of Dairy Science 34:1030–1033
    [Google Scholar]
  49. Williams R. A. D. 1971; Cell wall composition and enzymology of lactobacilli. Journal of Dental Research, 5:1104–1115
    [Google Scholar]
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