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Volume 137, Issue 3

Nucleotide sequence and taxonomic value of the major outer membrane protein gene of IOL-207 Free

Abstract

IOL-207 genomic DNA was hybridized with a 1·5 kb labelled DNA probe containing the 3′ region of the coding sequence for the major outer membrane protein (MOMP) of serovar L1. An 8·5 kb II fragment containing the complete MOMP gene was cloned into EMBL3. Two hybridizing RI fragments were sub-cloned into the ZAP II cloning vector and the resulting plasmids were used as templates for sequencing both strands of the MOMP gene. Computer taxonomic studies using the nucleotide and inferred amino acid sequence of the MOMP of IOL-207 and all known chlamydial MOMP sequences supported the designation of as a new species, but electron microscope studies suggested that the presence of pear-shaped elementary bodies (EBs) may not be a reliable taxonomic criterion.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society for General Microbiology 1991
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1991-03-01
2024-04-19
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Nucleotide sequence and taxonomic value of the major outer membrane protein gene of Chlamydia pneumoniae IOL-207
Microbiology 137, 465 (1991); https://doi.org/10.1099/00221287-137-3-465
/content/journal/micro/10.1099/00221287-137-3-465
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