1887

Abstract

IOL-207 genomic DNA was hybridized with a 1·5 kb labelled DNA probe containing the 3′ region of the coding sequence for the major outer membrane protein (MOMP) of serovar L1. An 8·5 kb II fragment containing the complete MOMP gene was cloned into EMBL3. Two hybridizing RI fragments were sub-cloned into the ZAP II cloning vector and the resulting plasmids were used as templates for sequencing both strands of the MOMP gene. Computer taxonomic studies using the nucleotide and inferred amino acid sequence of the MOMP of IOL-207 and all known chlamydial MOMP sequences supported the designation of as a new species, but electron microscope studies suggested that the presence of pear-shaped elementary bodies (EBs) may not be a reliable taxonomic criterion.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-3-465
1991-03-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/3/mic-137-3-465.html?itemId=/content/journal/micro/10.1099/00221287-137-3-465&mimeType=html&fmt=ahah

References

  1. Baehr W., Zhang Y. X., Joseph T., Su H., Nano F. E., Everett K. D. E., Caldwell H. D. 1988; Mapping antigenic domains expressed by Chlamydia trachomatis major outer membrane protein genes. Proceedings of the National Academy of Sciences of the United States of America 854000–4004
    [Google Scholar]
  2. Bavoil P., Ohlin A., Schächter J. 1984; Role of disulphide bonding in outer membrane structure and permeability in Chlamydia trachomatis . Infection and Immunity 44:479–485
    [Google Scholar]
  3. Campbell L. A., Kuo C. C., Grayston J. T. 1987; Characterization of the new Chlamydia agent, TWAR, as a unique organism by restriction endonuclease analysis and DNA-DNA hybridization. Journal of Clinical Microbiology 25:1911–1916
    [Google Scholar]
  4. Chi E. Y., Kuo C. C., Grayston J. T. 1987; Unique ultrastructure in the elementary body of Chlamydia sp. strain TWAR. Journal of Bacteriology 169:3757–3763
    [Google Scholar]
  5. Conlan J. W., Clarke I. N., Ward M. E. 1988; Epitope mapping with solid-phase peptides: identification of type-, subspecies-, species- and genus-reactive antibody binding domains on the major outer membrane protein of Chlamydia trachomatis . Molecular Microbiology 2:673–679
    [Google Scholar]
  6. Conlan J. W., Ferris S., Clarke I. N., Ward M. E. 1989; Surface-exposed epitopes on the major outer membrane protein of Chlamydia trachomatis defined with peptide antisera. Journal of General Microbiology 135:3219–3228
    [Google Scholar]
  7. Conlan J. W., Persson K., Newhall W. J., Ward M. E. 1990; Mapping of discontinuous epitopes on the major outer membrane protein of C trachomatis using synthetic peptides and monoclonal antibodies. In Chlamydial Infections81–84 Bowie W. R., Caldwell H. D., Jones R. P., Mardh P., Ridgway G. L., Schächter J., Stamm W. E., Ward M. E. Cambridge: Cambridge University Press;
    [Google Scholar]
  8. Cox R. L., Kuo C. C., Grayston J. T., Campbell L. A. 1988; Deoxyribonucleic acid relatedness of Chlamydia sp. strain TWAR to Chlamydia trachomatis and Chlamydia psittaci . International Journal of Systematic Bacteriology 38:265–268
    [Google Scholar]
  9. Dwyer R. St. C., Treharne J. D., Jones B. R., Herring J. 1972; Chlamydial infection. Results of micro-immunofluorescence tests for the detection of type-specific antibody in certain chlamydial infections. British Journal of Venereal Disease 48:452–459
    [Google Scholar]
  10. Felsenstein J. 1980 phylip 3.2 Manual California: University of California Herbarium;
    [Google Scholar]
  11. Forsey T., Darougar S., Treharne J. D. 1986; Prevalence in human beings of antibodies to Chlamydia IOL 207, an atypical strain of Chlamydia . Journal of Infection 12:145–152
    [Google Scholar]
  12. Grayston J. T., Kuo C. C., Wang S. P., Altman J. 1986; A new Chlamydia psittaci strain, TWAR, isolated in acute respiratory tract infections. New England Journal of Medicine 315:161–168
    [Google Scholar]
  13. Grayston J. T., Kuo C. C., Campbell L. A., Wang S. P. 1989a; Chlamydia pneumoniae sp. nov. for Chlamydia sp. strain TWAR. International Journal of Systematic Bacteriology 39:88–90
    [Google Scholar]
  14. Grayston J. T., Wang S. P., Kuo C. C., Campbell L. A. 1989b; Current knowledge on Chlamydia pneumoniae strain TWAR, an important cause of pneumonia and other acute respiratory diseases. European Journal of Clinical Microbiology and Infectious Diseases 8:191–202
    [Google Scholar]
  15. Hamilton P. T., Malinowski D. P. 1989; Nucleotide sequence of the major outer membrane protein from Chlamydia trachomatis serovar H. Nucleic Acids Research 17:8366
    [Google Scholar]
  16. Hatch T. P., Allan I., Pearce J. H. 1984; Structural and polypeptide differences between envelopes of infective and reproductive life cycle forms of Chlamydia spp. Journal of Bacteriology 157:13–20
    [Google Scholar]
  17. Hayes L. J., Pickett M. A., Conlan J. W., Everson J. S., Ward M. E., Clarke I. N. 1990; The major outer membrane proteins of Chlamydia trachomatis serovars A and B : intra-serovar amino acid changes do not alter specificities of serovar- and C subspecies-reactive antibody binding domains. Journal of General Microbiology 136:1559–1566
    [Google Scholar]
  18. Herring A. J., Tan T. W., Baxter S., Inglis N. F., Dunbar S. 1989; Sequence analysis of the major outer membrane protein gene of an ovine abortion strain of Chlamydia psittaci . FEMS Microbiology Letters 65:153–158
    [Google Scholar]
  19. Higgins D. G., Sharp P. M. 1988; Clustal; a package for performing multiple sequence alignments on a microcomputer. Gene 73:237–244
    [Google Scholar]
  20. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111–120
    [Google Scholar]
  21. Kuo C. C., Chen H. H., Wang S. P., Grayston J. T. 1986; Identification of a new group of Chlamydia psittaci strains called TWAR. Journal of Clinical Microbiology 6:1034–1037
    [Google Scholar]
  22. Kuo C. C., Chi E. Y., Grayston J. T. 1988; Ultrastructural study of entry of Chlamydia strain TWAR into HeLa cells. Infection and Immunity 56:1668–1672
    [Google Scholar]
  23. Marrie T. J., Grayston J. T., Wang S. P., Kuo C. C. 1987; Pneumonia associated with the TWAR strain of Chlamydia . Annals of Internal Medicine 106:507–511
    [Google Scholar]
  24. Moulder J. W. 1988; Characteristics of Chlamydiae . In Microbiology of Chlamydia3–19 Barron A. L. Florida: CRC Press;
    [Google Scholar]
  25. Pickett M. A., Ward M. E., Clarke I. N. 1987; Complete nucleotide sequence of the major outer membrane protein gene from Chlamydia trachomatis serovar L1. FEMS Microbiology Letters 42:185–190
    [Google Scholar]
  26. Pickett M. A., Everson J. S., Clarke I. N. 1988; Chlamydia psittaci ewe abortion agent: complete nucleotide sequence of the major outer membrane protein gene. FEMS Microbiology Letters 55:229–234
    [Google Scholar]
  27. Saikku P., Mattila K., Nieminen M. S., Huttunen J. K., Leinonen M., Ekman M. R., Mäkelä P. H., Valtonen V. 1988; Serological evidence of an association of a novel Chlamydia TWAR, with chronic coronary heart disease and acute myocardial infarction. Lancet 2:983–986
    [Google Scholar]
  28. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  29. Stephens R. S., Tam M. R., Kuo C. C., Nowinski R. C. 1982; Monoclonal antibodies to Chlamydia trachomatis : antibody specificities and antigen characterization. Journal of Immunology 128:1083–1089
    [Google Scholar]
  30. Stephens R. S., Mullenbach G., Sanchez-Pescador R., Agabian N. 1986; Sequence analysis of the major outer membrane protein gene from Chlamydia trachomatis serovar L2. Journal of Bacteriology 168:1277–1282
    [Google Scholar]
  31. Stephens R. S., Sanchez-Pescador R., , Wagar E. A., Inouye C., Urdea M. S. 1987; Diversity of Chlamydia trachomatis major outer membrane protein genes. Journal of Bacteriology 169:3879–3885
    [Google Scholar]
  32. Stephens R. S., Wagar E. A., Schoolnik G. K. 1988; High resolution mapping of serovar-specific and common antigenic determinants of the major outer membrane protein of Chlamydia trachomatis . Journal of Experimental Medicine 167:817–831
    [Google Scholar]
  33. Su H., Watkins N. G., Zhang Y. X., Caldwell H. D. 1990; Chlamydia trachomatis-host cell interactions : role of the chlamydial major outer membrane protein as an adhesin. Infection and Immunity 58:1017–1025
    [Google Scholar]
  34. Taylor-Robinson D., Ward M. E. 1989; Immunity to chlamydial infections and the outlook for vaccination. In Vaccines for Sexually Transmitted Diseases67–85 Meheus A., Spier R. E. London: Butterworths;
    [Google Scholar]
  35. Wang S. P., Grayston J. T. 1970; Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. American Journal of Ophthalmology 70:367–374
    [Google Scholar]
  36. Ward M. E., Murray A. 1984; Control mechanisms governing the infectivity of Chlamydia trachomatis for HeLa cells: mechanisms of endocytosis. Journal of General Microbiology 130:1765–1780
    [Google Scholar]
  37. Yuan Y., Zhang Y. X., Watkins N. G., Caldwell H. D. 1989; Nucleotide and deduced amino acid sequences for the four variable domains of the major outer membrane proteins of the 15 Chlamydia trachomatis serovars. Infection and Immunity 57:1040–1049
    [Google Scholar]
  38. Zhang H., Scholl R., Browse J., Somerville C. 1988; Double stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Research 16:1220
    [Google Scholar]
  39. Zhang Y. X., Stewart S., Joseph T., Taylor H. R., Caldwell H. D. 1987; Protective monoclonal antibodies recognize epitopes located on the major outer membrane protein of Chlamydia trachomatis . Journal of Immunology 138:575–581
    [Google Scholar]
  40. Zhang Y. X., Morrison S. G., Caldwell H. D., Baehr W. 1989; Cloning and sequence analysis of the major outer membrane protein genes of two Chlamydia psittaci strains. Infection and Immunity 57:1621–1625
    [Google Scholar]
  41. Zhang Y. X., Morrison S. G., Caldwell H. D. 1990; The nucleotide sequence of major outer membrane protein gene of Chlamydia trachomatis serovar F. Nucleic Acids Research 18:1061
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-3-465
Loading
/content/journal/micro/10.1099/00221287-137-3-465
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error