A cell surface/plasma membrane antigen of Free

Abstract

Antibody from BALB/cByJ mice immunized against a membranous fraction of agglutinated whole cells as well as the membranous fraction. Hybridoma techniques were used to isolate an IgM monoclonal antibody (mAb) designated 10G which agglutinated whole cells and reacted with the subcellular fraction. Yeast cells of 15 additional strains and isolates of and were also agglutinated by mAb 10G. The antigen was not detected on other fungi, including spp. and To determine the cellular location of the epitope to which mAb 10G is specific, freeze-substitution was compared with traditional chemical fixation methods in preparation of samples for immunocolloidal gold electron microscopy (IEM). With both fixation procedures, the antigen recognized by mAb 10G was found randomly and densely concentrated on the plasma membrane on exponential-phase yeast-form cells and had a patchy distribution on the cell wall surface. Association of the antigen with the plasma membrane was confirmed by IEM of isolated membranes. On developing hyphal cells, antigen appeared first on the plasma membrane and later on the cell wall surface. Treatment of yeast cells with -mercaptoethanol and Zymolyase before fixation removed the antigen from the surface but left the cytoplasmic antigen undisturbed. Treatment of yeast cells or solubilized antigen with heat or proteolytic enzymes (trypsin, Pronase B, proteinase K) did not remove or destroy the antigen, suggesting a non-protein nature of the epitope.

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1991-03-01
2024-03-29
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References

  1. Andaluz E., Guillen A., Larriba G. 1986; Preliminary evidence for a glucan acceptor in the yeast Candida albicans . Biochemical Journal 240:495–502
    [Google Scholar]
  2. Barnes J. L., Osgood R. W., Lee J. C., King R. D., Stein J. H. 1983; Host-parasite interactions in the pathogenesis of experimental renal candidiasis. Laboratory Investigation 49:460–467
    [Google Scholar]
  3. Brawner D. L., Cutler J. E. 1984; Variability in expression of a cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody. Infection and Immunity 43:966–972
    [Google Scholar]
  4. Brawner D. L., Cutler J. E. 1986; Ultrastructural and biochemical studies of two dynamically expressed cell surface determinants on Candida albicans . Infection and Immunity 51:327–336
    [Google Scholar]
  5. Brawner D. L., Cutler J. E. 1987; Cell surface and intracellular expression of two Candida albicans antigens during in vitro and in vivo growth. Microbial Pathogenesis 2:249–257
    [Google Scholar]
  6. Brown L. A., Chaffin W. L. 1981; Differential expression of cytoplasmic proteins during yeast bud and germ tube formation in Candida albicans . Canadian Journal of Microbiology 27:580–585
    [Google Scholar]
  7. Calderone R. A., Lehrer N., Segal E. 1984; Adherence of Candida albicans to buccal and vaginal epithelial cells : ultrastructural observations. Canadian Journal of Microbiology 30:1001–1007
    [Google Scholar]
  8. Calderone R. A., Linehan L., Wadsworth E., Sandberg A. L. 1988; Identification of C3d receptors on Candida albicans. Infection and Immunity 56:252–258
    [Google Scholar]
  9. Chavira J., Burnett T. J., Hageman J. H. 1984; Assaying proteinases with azocoll. Analytical Biochemistry 136:446–450
    [Google Scholar]
  10. Christensen M. S., Cirillo V. P. 1972; Yeast membrane vesicles: isolation and general characteristics. Journal of Bacteriology 110:1190–1205
    [Google Scholar]
  11. Cutler J. E., Friedman L., Milner K. C. 1972; Biological and chemical characterization of toxic substances from Candida albicans . Infection and Immunity 6:616–627
    [Google Scholar]
  12. Djaczenko W., Cassone A. 1971; Visualization of new ultrastructural components in the cell wall of Candida albicans with fixatives containing TAPO. Journal of Cell Biology 52:186–190
    [Google Scholar]
  13. Duran A., Bowers B., Cabib E. 1975; Chitin synthetase zymogen is attached to the yeast plasma membrane. Proceedings of the National Academy of Sciences of the United States of America 723952–3955
    [Google Scholar]
  14. Elorza M. V., Rico H., Gozalbo D., Sentandreu R. 1983; Cell wall composition and protoplast regeneration in Candida albicans . Antonie van Leeuwenhoek 49:457–469
    [Google Scholar]
  15. Fuhrmann G. F., Wehrli E., Boehm C. 1974; Preparation and identification of yeast plasma membrane vesicles. Biochimica et Biophysica Acta 363:295–310
    [Google Scholar]
  16. Glee P. M., Russell P. J., Welsch J. A., Pratt J. C., Cutler J. E. 1987; Methods for DNA extraction from Candida albicans. Analytical Biochemistry 164:207–213
    [Google Scholar]
  17. Hazen K. C., Cutler J. E. 1979; Autoregulation of germ tube formation by Candida albicans . Infection and Immunity 24:661–666
    [Google Scholar]
  18. Hazen K. C., Cutler J. E. 1982; Optimal conditions for breaking medically important yeasts by an inexpensive and simple method. Mycopathologia 80:113–116
    [Google Scholar]
  19. Hazen K. C., Cutler J. E. 1983; Effect of cobalt and morphogenic autoregulatory substance (MARS) on morphogenesis of Candida albicans. Experimental Mycology 7:182–187
    [Google Scholar]
  20. Hoch H. C. 1986; Freeze-substitution of fungi. In Ultrastructure Techniques for Microorganisms183–212 Aldrich H. C., Todd W. J. New York: Plenum Press;
    [Google Scholar]
  21. Hoch H. C., Howard R. J. 1980; Ultrastructure of freeze-substituted hyphae of the basidiomycete Laetisaria arvalis . Proto-plasma 103:281–297
    [Google Scholar]
  22. Hoch H. C., Howard R. J. 1981; Conventional chemical fixations induce artifactual swelling of dolipore septa. Experimental Mycology 5:167–172
    [Google Scholar]
  23. Hopwood V., Poulain D., Fortier B., Evans G., Vernes A. 1986; A monoclonal antibody to a cell wall component of Candida albicans . Infection and Immunity 54:222–227
    [Google Scholar]
  24. Hudspeth M. E. S., Shumard D. S., Tatti K. M., Grossman L. I. 1980; Rapid purification of yeast mitochondrial DNA in high yield. Biochimica et Biophysica Acta 610:221–228
    [Google Scholar]
  25. King R. D., Lee J. C., Morris A. L. 1980; Adherence of Candida albicans and other Candida species to mucosal epithelial cells. Infection and Immunity 27:667–674
    [Google Scholar]
  26. Korn E. D., Northcote D. H. 1960; Physical and chemical properties of polysaccharides and glycoproteins of the yeast-cell wall. Biochemical Journal 75:12–17
    [Google Scholar]
  27. Kuruganti U., Henderson L. A., Garner R. E., Asofsky R., Baker P. J., Domer J. E. 1988; Nonspecific and Candidaspecific immune responses in mice suppressed by chronic administration of anti-μ. Journal of Leukocyte Biology 44:422–433
    [Google Scholar]
  28. Lee K. L., Buckley H. R., Campbell C. C. 1975; An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans . Sabouraudia 13:148–153
    [Google Scholar]
  29. Lehrer N., Segal E., Cihlar R. L., Calderone R. A. 1986; Pathogenesis of vaginal candidiasis : studies with a mutant which has reduced ability to adhere in vitro. Journal of Medical and Veterinary Mycology 24:127–131
    [Google Scholar]
  30. Levitz S. M., Lyman C. A., Murata T., Sullivan J. A., Mandell G. L., Diamond R. D. 1987; Cytosolic calcium changes in individual neutrophils stimulated by opsonized and unopsonized Candida albicans hyphae. Infection and Immunity 55:2783–2788
    [Google Scholar]
  31. Maisch P. A., Calderone R. A. 1981; Role of surface mannan in the adherence of Candida albicans to fibrin-platelet clots formed in vitro . Infection and Immunity 32:92–97
    [Google Scholar]
  32. Marriott M. S. 1977; Mannan-protein location and biosynthesis in plasma membranes from the yeast form of Candida albicans . Journal of General Microbiology 103:51–59
    [Google Scholar]
  33. Novick P., Schekman R. 1983; Export of major cell surface proteins is blocked in yeast secretory mutants. Journal of Cell Biology 96:541–547
    [Google Scholar]
  34. Novick P., Ferro S., Schekman R. 1981; Order of events in the yeast secretory pathway. Cell 25:461–469
    [Google Scholar]
  35. Ollert M. W., Calderone R. A. 1990; A monoclonal antibody that defines a surface antigen on Candida albicans hyphae cross-reacts with yeast cell protoplasts. Infection and Immunity 58:625–631
    [Google Scholar]
  36. Poulain D., Cailliez J. F., Dubremetz J. F. 1989; Secretion of glycoproteins through the cell wall of Candida albicans . European Journal of Cell Biology 50:94–99
    [Google Scholar]
  37. Raff M. C., Megson M., Owen J. J. T., Cooper M. D. 1976; Early production of intracellular IgM by B-lymphocyte precursors in mouse. Nature; London: 259224–226
    [Google Scholar]
  38. Rajasingham K. C., Cawson R. A. 1984; Plasmalemmasomes and lomasomes in Candida albicans . Cytobios 40:21–25
    [Google Scholar]
  39. Shepherd M. G. 1987; Cell envelope of Candida albicans. CRC Critical Reviews in Microbiology 15:7–25
    [Google Scholar]
  40. Smail E. H., Jones J. M. 1984; Demonstration and solubilization of antigens expressed primarily on the surfaces of Candida albicans germ tubes. Infection and Immunity 45:74–81
    [Google Scholar]
  41. Spurr A. R. 1969; A low viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26:31–38
    [Google Scholar]
  42. Sundstrom P. M., Kenny G. E. 1984; Characterization of antigens specific to the surface of germ tubes of Candida albicans by immunofluorescence. Infection and Immunity 43:850–855
    [Google Scholar]
  43. Takamiya H., Vogt A., Batsford S., Kuttin E. S., Muller J. 1985; Further studies on the immunoelectronmicroscopic localization of polysaccharide antigen on ultra-thin sections of Candida albicans . Mykosen 28:17–32
    [Google Scholar]
  44. Tronchin G., Vernes A., Poulain D. 1984; Cytochemical and ultrastructural studies of Candida albicans III. Evidence for modification of the cell wall coat during adherence to human buccal epithelial cells. Archives of Microbiology 139:221–224
    [Google Scholar]
  45. Tsai P.-K., Ballou L., Esmon B., Shekman R., Ballou C. E. 1984; Isolation of glucose-containing high-mannose glycoprotein core oligosaccharides. Proceedings of the National Academy of Sciences of the United States of America 816340–6343
    [Google Scholar]
  46. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides. Extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
  47. Wills J. W., Lasker B. A., Sirotkin K., Riggsby W. S. 1984; Repetitive DNA of Candida albicans: nuclear and mitochondrial components. Journal of Bacteriology 157:918–924
    [Google Scholar]
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