1887

Abstract

Summary

A selection of adenosine analogues was tested for their ability to trigger germination of NCIB 8122 spores. The germination-inducing activity was governed by the structural properties of the sugar rather than the base moieties of the nucleosides. Among the sugar-modified analogues, only those containing a 2′-deoxy-D-ribose moiety promoted spore germination. Requirements for a specific molecular structure of the base were not clearly identified, although the highest activity was observed when substituents were inserted at position 6 of the purine ring. All the base-modified analogues, even those such as coformycin and 2′-deoxycoformycin with an expanded base ring, retained the germination-inducing activity of adenosine. However, of the two 2′-deoxycoformycin diastereoisomers characterized by an asymmetric carbon atom at position 8 of the homopurine ring, only the 8 -isomer induced germination, thus indicating that stereospecific configuration of the inducer, at least in the case of 2′-deoxycoformycin, appears to be essential for the initiation of spore germination. The differences in the germination-inducing activity of the various analogues tested were not affected significantly by spore activation at different temperatures, although the higher the activation temperature, the lower was the concentration of each analogue required for maximum germination.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-2-399
1991-02-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/2/mic-137-2-399.html?itemId=/content/journal/micro/10.1099/00221287-137-2-399&mimeType=html&fmt=ahah

References

  1. Foster S. J., Johnstone K. 1990; Pulling the trigger: the mechanism of bacterial spore germination. Molecular Microbiology 4:137–141
    [Google Scholar]
  2. Hashimoto T., Frieben W. R., Conti S. F. 1972; Kinetics of germination of heat-injured Bacillus cereus spores.. In Spores V409–415 Halvorson H. O., Hanson R., Campbell L. L. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Hills G. M. 1949a; Chemical factors in the germination of spore-bearing aerobes. The effect of amino acids on the germination of Bacillus anthracis and its replacement by adenosine. Biochemical Journal 45:353–362
    [Google Scholar]
  4. Hills G. M. 1949b; Chemical factors in the germination of spore-bearing aerobes. The effect of amino acids on the germination of Bacillus anthracis with some observations on the relation of optical form to biological activity. Biochemical Journal 45:363–370
    [Google Scholar]
  5. Janssen F. W., Lund A. J., Anderson L. E. 1958; Colorimetric assay of dipicolinic acid in bacterial spores. Science 127:26–27
    [Google Scholar]
  6. Keynan A. 1978; Spore structure and its relations to resistance, dormancy, and germination. In Spores VII43–53 Chambliss G., Vary J. C. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  7. Keynan A., Sandler N. 1983; Spore research in historical perspective. In The Bacterial Spore 21–48 Hurst A., Gould G. W. London: Academic Press;
    [Google Scholar]
  8. Kutima P. M., Foegeding P. M. 1987; Involvement of the spore coat in germination of Bacillus cereus T spores.. Applied and Environmental Microbiology 53:47–52
    [Google Scholar]
  9. Lawrence L. N. 1955a; The cleavage of adenosine by spores of Bacillus cereus. Journal of Bacteriology 70:577–582
    [Google Scholar]
  10. Lawrence L. N. 1955b; The relationship between the cleavage of purine ribosides by bacterial spores and germination of the spores. Journal of Bacteriology 70:583–587
    [Google Scholar]
  11. Powell J. F., Hunter J. R. 1955; Spore germination in the genus Bacillus: the modification of germination requirements as a result of preheating.. Journal of General Microbiology 13:59–67
    [Google Scholar]
  12. Powell J. F., Hunter J. R. 1956; Adenosine deaminase and ribosidase in spores of Bacillus cereus. Biochemical Journal 62:381–387
    [Google Scholar]
  13. Scott I. R., Ellar D. J. 1978; Study of calcium dipicolinate release during bacterial spore germination by using a new sensitive assay for dipicolinate. Journal of Bacteriology 135:133–137
    [Google Scholar]
  14. Senesi S., Falcone G., Ipata P. L., Felicioli R. A. 1974; Inhibition of phosphodiesterases from Bacillus subtilis by nucleoside triphosphates.. Biochemistry 13:5008–5011
    [Google Scholar]
  15. Senesi S., Falcone G., Mura U., Sgarrella F., Ipata P. L. 1977; Adenosine phosphorylase from vegetative forms and free spores of Bacillus subtilis: properties and possible physiological role.. In Spore Research 1976 I311–333 Barker A. N., Wolf L. J., Ellar D. J., Dring G. J., Gould G. W. London: Academic Press;
    [Google Scholar]
  16. Setlow P. 1983; Germination and outgrowth. In The Bacterial Spore 2211–254 Hurst A., Gould G. W. London: Academic Press;
    [Google Scholar]
  17. Shacter S. M., Hashimoto T. 1975; Bimodal kinetics of germination of Bacillus cereus T spores.. In Spores VI531–538 Gerhardt P., Costilow R. N., Sadoff H. L. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  18. Shibata H., Ohnishi N., Takeda K, Fukunaga H., Shimamura K., Yasunobu E., Tani I. 1985; Germination of Bacillus cereus spores induced by purine ribosides and their analogs: effects of modification of base and sugar moieties of purine nucleosides on germination-inducing activity.. Canadian Journal of Microbiology 32:186–189
    [Google Scholar]
  19. Sogin M. L., MacCall W. A., Ordal Z. J. 1972; Effect of heat activation conditions on the germinal response of Bacillus cereus T spores.. In Spores V363–367 Halvorson H. O., Hanson R., Campbell L. L. Washington DC: American Society for Microbiology;
    [Google Scholar]
  20. Warren S. C., Gould G. W. 1968; Bacillus cereus spore germination: absolute requirement for amino acid.. Biochimica et Biophysica Ada 170:341–350
    [Google Scholar]
  21. Warth A. D. 1978; Relationship between the heat resistance of spores and the optimum growth temperatures of Bacillus species.. Journal of Bacteriology 134:699–705
    [Google Scholar]
  22. Weinberger S., Evenchik Z., Hertman I. 1980; Utilization of purine nucleosides by germinating Bacillus cereus 569 spores.. Journal of General Microbiology 118:223–228
    [Google Scholar]
  23. Yousten A. A. 1975; Germination of Bacillus cereus endospores: a proposed role for heat shock and nucleosides.. Canadian Journal of Microbiology 21:1192–1197
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-2-399
Loading
/content/journal/micro/10.1099/00221287-137-2-399
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error