1887

Abstract

Summary

mutants defective in myxospore germination have been isolated both by a selective and by a non-selective method after UV or Tn-induced mutagenesis. The ability of these mutants to germinate in germinant solutions other than those used for their isolation has been tested. Six of seven mutants isolated behaved as germination-defective in all germinants. Germination of the seventh mutant was conditional on the germinant used, being normal in Casamino acids but defective in a Casitone-based medium. Genetic analysis of the four mutant strains carrying Tn- insertions revealed that the transposon had disrupted a different locus in each mutant, so that the four mutants defined four unlinked loci involved in the germination process (). Strain MR307 was studied in more detail. Cloning of the gene affected in this mutant, , and construction of merodiploids revealed that the wild-type allele is dominant over the mutated one. construction of fusions allowed study of expression throughout the life cycle, revealing that the gene affected by insertion at ΩMR307 is developmentally regulated.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-2-389
1991-02-01
2021-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/2/mic-137-2-389.html?itemId=/content/journal/micro/10.1099/00221287-137-2-389&mimeType=html&fmt=ahah

References

  1. Avery L., Kaiser D. 1983; In situ transposon replacement and isolation of a spontaneous tandem genetic duplication. Molecular and General Genetics 19:99–109
    [Google Scholar]
  2. Balsalobre J. M., Ruiz-Vázquez R. M., Murillo F. J. 1987; Light induction of gene expression in Myxococcus xanthus . Proceedings of the National Academy of Sciences of the United States of America 842359–2362
    [Google Scholar]
  3. Bretscher A. P., Kaiser D. 1978; Nutrition of Myxococcus xanthus, a fruiting myxobacterium.. Journal of Bacteriology 133:763–768
    [Google Scholar]
  4. Casadaban M. J., Cohen S. N. 1980; Analysis of gene control signals by DNA fusion and cloning in Escherichia coli . Journal of Molecular Biology 138:179–207
    [Google Scholar]
  5. Dworkin M., Kaiser D. 1985; Cell interactions in myxobacterial growth and development. Science 230:18–24
    [Google Scholar]
  6. Elías M., Murillo F. J. 1991; Induction of germination in Myxococcus xanthus fruiting body spores.. Journal of General Microbiology 137:381–388
    [Google Scholar]
  7. Gill R. E., Cull M. G., Fly S. 1988; Genetic identification and cloning of a gene required for developmental cell interactions in Myxococcus xanthus . Journal of Bacteriology 170:5279–5288
    [Google Scholar]
  8. Hagen D. C, Bretscher A. P., Kaiser D. 1978; Synergism between morphogenetic mutants of Myxococcus xanthus . Developmental Biology 64:284–296
    [Google Scholar]
  9. Hodgkin J., Kaiser D. 1977; Cell-to-cell stimulation of movement in nonmotile mutants of Myxococcus . Proceedings of the National Academy of Sciences of the United States of America 742938–2942
    [Google Scholar]
  10. Kaiser D. 1984; Genetics of myxobacteria. Myxobacteria Development and Cell Interactions163–184 Rosenberg E. New York: Springer-Verlag;
    [Google Scholar]
  11. Kroos L., Kaiser D. 1984; Construction of Tn5-/ac, a transposon that fuses lacZ expression to exogenous promoters, and its introduction into Myxococcus xanthus . Proceedings of the National Academy of Sciences of the United States of America 815816–5820
    [Google Scholar]
  12. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Rosner J. L. 1972; Formation induction and curing of bacteriophage PI lysogens. Virology 48:679–689
    [Google Scholar]
  14. Ruiz-Vázquez R. M., Murillo F. J. 1984; Abnormal motility and fruiting behavior of Myxococcus xanthus bacteriophage-resistant strains induced by a clear-plaque mutant of bacteriophage Mx8.. Journal of Bacteriology 160:818–821
    [Google Scholar]
  15. Shimkets L. J. 1987; Control of morphogenesis in myxobacteria. CRC Critical Reviews in Microbiology 14:195–227
    [Google Scholar]
  16. Shimkets L. J., Gill R. E., Kaiser D. 1983; Developmental cell interactions in Myxococcus xanthus and the spoC locus.. Proceedings of the National Academy of Sciences of the United States of America 80:1406–1410
    [Google Scholar]
  17. Stephens K., Kaiser D. 1987; Genetics of gliding motility in Myxococcus xanthus: molecular cloning of the mgl locus.. Molecular and General Genetics 207:256–266
    [Google Scholar]
  18. Yee T., Inouye M. 1981; Reexamination of the genome size of myxobacteria, including the use of a new method for genome size analysis. Journal of Bacteriology 145:1257–1265
    [Google Scholar]
  19. Zusman D. R. 1984; Cell-cell interactions and development in Myxococcus xanthus . Quarterly Review of Biology 50:119–138
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-2-389
Loading
/content/journal/micro/10.1099/00221287-137-2-389
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error