1887

Abstract

Summary

The coding characteristics of four plasmids expressing a protein (BCP) which comigrates with bacterioferritin were examined and the nucleotide sequence of a common 1985 bp segment from the 53 min region of the linkage map was determined. Three open reading-frames ( and ) were detected, and orf2 (bcp, 156 amino acid codons) appeared to encode the bacterioferritin comigratory protein, BCP. The translation product of (205 amino acid codons) resembled the iron-sulphur protein component (DMS B subunit) of the anaerobic dimethylsulphoxide reductase complex of .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-2-361
1991-02-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/2/mic-137-2-361.html?itemId=/content/journal/micro/10.1099/00221287-137-2-361&mimeType=html&fmt=ahah

References

  1. Andrews S. C., Harrison P. M., Guest P. M. 1989; Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12.. Journal of Bacteriology 171:3940–3947
    [Google Scholar]
  2. Bachmann B. J. 1990; Linkage map of Escherichia coli K-12. , edition 8. Microbiological Reviews 54:130–197
    [Google Scholar]
  3. Beinert H. 1990; Recent developments in the field of iron-sulfur proteins. The FASEB Journal 4:2483–2491
    [Google Scholar]
  4. Biggin M. D., Gibson T. J., Hong G. F. 1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences of the United States of America 80:3963–3965
    [Google Scholar]
  5. Bilous P. T., Cole S. T., Anderson W. F., Weiner J. H. 1988; Nucleotide sequence of the dmsABC operon encoding the anaerobic dimethylsulphoxide reductase of Escherichia coli . Molecular Microbi-ology 2:785–795
    [Google Scholar]
  6. Busby S. 1986; Catabolite repression and related processes. Symposia of the Society for General Microbiology 39:51–77
    [Google Scholar]
  7. Clarke L., Carbon J. 1976; A colony bank containing synthetic ColEl hybrid plasmids representative of the entire E . coli genome Cell 9:91–99
    [Google Scholar]
  8. Darlison M. G., Guest J. R. 1984; Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli . Biochemical Journal 223:507–517
    [Google Scholar]
  9. Griggs D. W., Konisky J. 1989; Mechanism for iron-regulated transcription of the Escherichia coli cir gene : metal-dependent binding of Fur protein to the promoters.. Journal of Bacteriology 171:1048–1054
    [Google Scholar]
  10. Kohara Y., Akiyama K., Isono K. 1987; Physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495–508
    [Google Scholar]
  11. MacDonald H., Pope N. R., Cole J. A. 1985; Isolation, characterization and complementation analysis of nirB mutants of Escherichia coli deficient only in NADH-dependent nitrite reductase activity.. Journal of General Microbiology 131:2771–2782
    [Google Scholar]
  12. Messing J. 1983; New M13 vectors for cloning. Methods in Enzymology 101:20–78
    [Google Scholar]
  13. Neidhardt F. C., Vaughn V., Phillips T. A., Bloch P. L. 1983; Gene-protein index of Escherichia coli K-12.. Microbiological Reviews 47:231–284
    [Google Scholar]
  14. O’Farrell P. H. 1975; High resolution two-dimensional electro-phoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  15. Parker J. 1984; Identification of the purC gene product of Escherichia coli . Journal of Bacteriology 157:712–717
    [Google Scholar]
  16. Richaud F., Richaud C., Ratet P., Patte J.-C. 1986; Chromosomal location and nucleotide sequence of the Escherichia coli dap A gene.. Journal of Bacteriology 166:297–300
    [Google Scholar]
  17. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  19. Schwartz R.M., Dayhoff M. O. 1978; Matrices for detecting distant relationships. Atlas of Protein Sequence and Structure 53353–358 Dayhoff M. O. Washington, DC: National Biomedical Research Foundation;
    [Google Scholar]
  20. Spiro S., Guest J. R. 1990; FNR and its role in oxygen-regulated gene expression in Escherichia coli . FEMS Microbiology Reviews 75:399–428
    [Google Scholar]
  21. Staden R. 1982; An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Research 10:2951–2961
    [Google Scholar]
  22. Staden R. 1984; Graphic methods to determine the function of nucleic acid sequences. A summary of analyseq options. Nucleic Acids Research 12:521–538
    [Google Scholar]
  23. Tabor S., Richardson C. C. 1987; DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 84:4767–4771
    [Google Scholar]
  24. Vieira J., Messing J. 1987; Production of single-stranded plasmid DNA. Methods in Enzymology 153:3–34
    [Google Scholar]
  25. Yariv J., Kalb A. J., Sperling R., Bauminger E. R., Cohen S. G., Ofer S. 1981; The composition and structure of bacterioferritin of Escherichia coli . Biochemical Journal 197:171–175
    [Google Scholar]
  26. Zhang H., Scholl R., Browse J., Somerville C. 1988; Double stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Research 16:1220
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-2-361
Loading
/content/journal/micro/10.1099/00221287-137-2-361
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error