Aspergillus nidulans produces an extracellular β-d-fructofuranoside fructohydrolase (invertase) when grown on a medium containing the β-fructofuranosides sucrose or raffinose, indicating that synthesis is subject to induction by the substrate. On a growth medium containing sucrose, production was maximal at 15 h in cultures incubated at 28 C°. After this time the level of detectable invertase in the cultures declined. A proportion of the enzyme was secreted during the linear growth phase of the fungus. Various sugars were investigated for induction of invertase, but only the two β-fructofuranosides induced high production levels; with the other sugars, the enzyme was produced only at a low constitutive level. Mycelium grown under repressive conditions (1% glucose), rapidly produced invertase when transferred to sucrose-containing medium. After 80 min the invertase level in these cultures was 26-fold higher than the constitutive level. The repressive effect of other sugars, e.g. glucose and xylose, on invertase production was also demonstrated in this experimental system.
AndresI.,
PeberdyJ. F.1974; The production of invertase in Aspergillus nidulans with reference to the effects of glucose and sucrose. Microbios 10:15–23
BradfordM. M.1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
CarlsonM.,
BotsteinD.1982; Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertase. Cell 28:145–154
CasanovaM,
MartinezJ. P.,
GilM. L.,
SentandreuR.,
Ruiz-HerreraJ.1987; Different molecular forms of invertase in the slime variant of Neurospora crassa: comparison with the wild-type strain. Journal of General Microbiology 133:2447–2456
EilersF. I.,
AllenJ.,
HillE. P.,
SussmanA. S.1964; Localization of disaccharidases in extracts of Neurospora after electrophoresis in polyacrylamide gels. Journal of Histochemistry and Cytochemistry 12:448–450
EsmonP. C.,
EsmonB. E.,
SchauerI. E.,
TaylorA.,
SchekmanR.1987; Structure, assembly, and secretion of octameric invertase. Journal of Biological Chemistry 262:4387–4394
GabrielO.,
WangS. F.1969; Determination of enzymatic activity in polyacrylamide gels. I. Enzymes catalyzing the conversion of nonreducing substrates to reducing products. Analytical Biochemistry 27:545–555
GalbraithJ. C.,
SmithJ. E.1969; Changes in activity of certain enzymes of the tricarboxylic acid cycle and the glyoxylate cycle during the initiation of conidiation of Aspergillus niger
. Canadian Journal of Microbiology 15:1207–1212
GasconS.,
NeumannN. P.,
LampenO.1968; Comparative study of the properties of the purified internal and external invertases from yeast. Journal of Biological Chemistry 243:1573–1577
GuptaA. K.,
BhatiaI. S.1982; Glucofructosan biosynthesis in Fusarium oxysporum: regulation and substrate specificity of fructosyl transferase and invertase. Phytochemistry 21:1249–1253
HackelR. A.1975; Genetic control of invertase formation in Saccharomyces cerevisae I. Isolation and characterization of mutants affecting sucrose utilization. Molecular and General Genetics 140:361–370
HackelR. A.,
KhanN. A.1978; Genetic control of invertase formation in Saccharomyces cerevisiae II. Isolation and characterization of mutants conferring invertase hyperproduction in strain EK-6B carrying the SUC3 Gene. Molecular and General Genetics 164:295–302
KratkyZ.,
BielyP.,
BauerS.1975; Mechanism of 2-deoxy-dglucose inhibition of cell-wall polysaccharide and glycoprotein biosynthesis in Saccharomyces cerevisiae
. European Journal of Biochemistry 54:459–167
PerlmanD.,
HalvorsonH. O.,
CannonL. E.1982; Presecretory and cytoplasmic invertase polypeptides encoded by distinct mRNAs derived from the same structural gene differ by a signal sequence. Proceedings of the National Academy of Sciences of the United States of America79781–785
RodriguezL.,
LampenJ. O.,
MackayV. L.1981; SUC1 gene of Saccharomyces: a structural gene for the large (glycoprotein) and small (carbohydrate-free) forms of invertase. Molecular and Cellular Biology 1:469–474
SarokinL.,
CarlsonM.1986; Short repeated elements in the upstream regulatory region of the SUC2 gene of Saccharomyces cerevisiae
. Molecular and Cellular Biology 6:2324–2333
TrevithickJ. R.,
MetzenbergR. L.1966; Molecular sieving by Neurospora cell walls during secretion of invertase isoenzymes. Journal of Bacteriology 92:1010–1015