The development of specific rRNA-derived oligonucleotide probes for , the causative agent of chancroid Free

Abstract

Summary

Part of a ribosomal ribonucleic acid (rRNA) cistron of was enzymically amplified using conserved primers within the rRNA molecules, cloned in a plasmid vector, and sequenced. From the nucleotide sequence, eight oligonucleotides complementary to different regions in the 16S and 23S rRNA molecules were selected, chemically synthesized, and used as hybridization probes. Hybridization experiments with at least 41 strains and 13 or 14 non- strains revealed that all eight oligonucleotide probes were highly reliable and completely specific for strains. Comparisons of 16S rRNA sequences confirm that is a member of the though not closely related to other species in this family.

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1991-02-01
2024-03-29
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References

  1. Albritton W. L. 1989; Biology of Haemophilus ducreyi. Microbiological Reviews 53:377–389
    [Google Scholar]
  2. Albritton W. L., Setlow J. K., Thomas M., Sottnek F. O. 1986; Relatedness within the family Pasteurellaceae as determined by genetic transformation. International Journal of Systematic Bacteriology 36:103–106
    [Google Scholar]
  3. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  4. Brosius J., Dull T. J., Sleeter D. D., Noller H. F. 1981; Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. Journal of Molecular Biology 148:107–127
    [Google Scholar]
  5. Carbon P., Ebel J.-P., Ehresmann C. 1981; The sequence of the ribosomal 16S RNA from Proteus vulgaris: sequence comparison with E. coli 16S RNA and its use in secondary structure model building. Nucleic Acids Research 9:2325–2333
    [Google Scholar]
  6. Carlone G. M., Schalla W. O., Moss C. W., Ashley D. L., Fast D. M., Holler J. S., Plikaytis B. D. 1988; Haemophilus ducreyi isoprenoid quinone content and structure determination. International Journal of Systematic Bacteriology 38:249–253
    [Google Scholar]
  7. Casin I., Grimont F., Grimont P. A. D., Sanson-Le Pors M.-J. 1985; Lack of deoxyribonucleic acid relatedness between Haemophilus ducreyi and other Haemophilus species. International Journal of Systematic Bacteriology 35:23–25
    [Google Scholar]
  8. Chuba P. J., Bock R., Graf G., Adam T., Gobel U. 1988; Comparison of 16S rRNA sequences from the family Pasteurellaceae : phylogenetic relatedness by cluster analysis. Journal of General Microbiology 134:1923–1930
    [Google Scholar]
  9. Dams E., Hendriks L., Van de Peer Y., Neefs J.-M., Smits G., Vandenbempt I., De Wachter R. 1988; Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Research 16:r87–r173
    [Google Scholar]
  10. De Ley J., Mannheim W., Mutters R., Piechulla K., Tytgat R., Segers P., Bisgaard M., Frederiksen W., Hinz K.-H., Vanhoucke M. 1990; Inter- and intrafamilial similarities of rRNA cistrons of the Pasteurellaceae. International Journal of Systematic Bacteriology 40:128–139
    [Google Scholar]
  11. Denys G. A., Chapel T. A., Jeffries C. D. 1978; An indirect fluorescent antibody technique for Haemophilus ducreyi. Health Laboratory Science 15:128–132
    [Google Scholar]
  12. Dorsch M., Moreno E., Stackebrandt E. 1989; Nucleotide sequence of the 16S rRNA from Brucella abortus. Nucleic Acids Research 17: 1765
    [Google Scholar]
  13. Edwards U., Rogall T., Blocker H., Emde M., Bottger E. C. 1989; Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Research 17:7843–7853
    [Google Scholar]
  14. Gutell R. R., Fox G. E. 1988; A compilation of large subunit RNA sequences presented in a structural format. Nucleic Acids Research 16:r175–r269
    [Google Scholar]
  15. Haun G., Göbel U. 1987; Oligonucleotide probes for genus-,species- and subspecies-specific identification of representatives of the genus Proteus. FEMS Microbiology Letters 43:187–193
    [Google Scholar]
  16. Höpfl P., Ludwig W., Schleifer K. H., Larsen N. 1989; The 23S ribosomal RNA higher-order structure of Pseudomonas cepacia and other prokaryotes. European Journal of Biochemistry 185:355–364
    [Google Scholar]
  17. Huss V. A. R., Sogin M. L. 1989; Primary structure of the Chlorella vulgaris small subunit ribosomal RNA coding region. Nucleic Acids Research 17:1255
    [Google Scholar]
  18. Kahn M., Kolter R., Thomas C., Figurski D., Meyer R., Remaut E., Helinski D. R. 1979; Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, RK2. Methods in Enzymology 68:268–280
    [Google Scholar]
  19. Kilian M. 1976; A taxonomic study of the genus Haemophilus, with the proposal of a new species. Journal of General Microbiology 93:9–62
    [Google Scholar]
  20. Kilian M., Biberstein E. L. 1984; Genus II. Haemophilus Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1917, 561al. Bergey’s Manual of Systematic Bacteriology 1558–569 Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  21. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S rRNA sequences for phylogenetic analyses. Proceedings of the National Academy of Sciences of the United States of America 826955–6959
    [Google Scholar]
  22. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Marmur J. A. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  24. Medlin L., Elwood H. J., Stickel S., Sogin M. L. 1988; The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71:491–499
    [Google Scholar]
  25. Morse S. A. 1989; Chancroid and Haemophilus ducreyi. Clinical Microbiology Reviews 2:137–157
    [Google Scholar]
  26. Museyi K., Van Dyck E., Vervoort T., Taylor D., Hoge C., Plot P. 1988; Use of an enzyme immunoassay to detect serum IgG antibodies to Haemophilus ducreyi. Journal of Infectious Diseases 157:1039–1043
    [Google Scholar]
  27. Parsons L. M., Shayegani M., Waring A. L., Bopp L. H. 1989; DNA probes for the identification of Haemophilus ducreyi. Journal of Clinical Microbiology 27:1441–1445
    [Google Scholar]
  28. Ronald A. R., Albritton W. L. 1984; Chancroid and Haemophilus ducreyi. Sexually Transmitted Diseases385–393 Holmes K. K., Mardh P. A., Sparling P. F., Wiesner P. J. New York: McGraw-Hill;
    [Google Scholar]
  29. Rossau R., Heyndrickx L., Van Heuverswyn H. 1988; Nucleotide sequence of a 16S ribosomal RNA gene from Neisseria gonorrhoeae. Nucleic Acids Research 16:6227
    [Google Scholar]
  30. Rossau R., Vanmechelen E., De Ley J., Van Heuverswyn H. 1989; Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA. Journal of General Microbiology 135:1735–1745
    [Google Scholar]
  31. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 745463–5467
    [Google Scholar]
  32. Schalla W. O., Sanders L. L., Schmid G. P., Tam M. R., Morse S. A. 1986; Use of dot-immunobinding and immunofluorescence assays to investigate clinically suspected cases of chancroid. Journal of Infectious Diseases 153:879–887
    [Google Scholar]
  33. Stackebrandt E., Murray R. G. E., Truper H. G. 1988; Proteobacteria classis nov., a name for the phylogenetic taxon that includes the ‘purple bacteria and their relatives’. International Journal of Systematic Bacteriology 38:321–325
    [Google Scholar]
  34. Tenover F. C. 1988; Diagnostic deoxyribomicleic acid probes for infectious diseases. Clinical Microbiology Reviews 1:82–101
    [Google Scholar]
  35. Toschka H. Y., Höpfl P., Ludwig W., Schleifer K. H., Ulbrich N., Erdmann V. A. 1987; Complete nucleotide sequence of a 23S ribosomal RNA gene from Pseudomonas aeruginosa. Nucleic Acids Research 15:7182
    [Google Scholar]
  36. Toschka H. Y., Höpfl P., Ludwig W., Schleifer K. H., Ulbrich N., Erdmann V. A. 1988; Complete nucleotide sequence of a 16S ribosomal RNA gene from Pseudomonas aeruginosa. Nucleic Acids Research 16:2348
    [Google Scholar]
  37. Viscidi R. P., Yolken R. G. 1987; Molecular diagnosis of infectious diseases by nucleic acid hybridization. Molecular and Cellular Probes 1:3–14
    [Google Scholar]
  38. Woese C. R. 1987; Bacterial evolution. Microbiological Reviews 51:221–271
    [Google Scholar]
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