1887

Abstract

Summary

The iron uptake systems of pathogenic bacteria provide potential targets for immunological intervention. We have partially purified the high molecular mass, iron-regulated outer membrane proteins (IROMPs) from and used them to prepare a panel of monoclonal antibodies (mAbs). Five mAbs reacted with an 85 kDa IROMP separated by SDS-PAGE, but gave only low-level binding to whole cells by immunogold electron microscopy. However, iodination of whole cells indicated that the 85 kDa IROMP is surface-exposed. The mAbs were only cross-reactive with clinical isolates representing eight of the 17 International Antigenic Typing Scheme serotypes of , suggesting significant heterogeneity with respect to this IROMP.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-2-227
1991-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/2/mic-137-2-227.html?itemId=/content/journal/micro/10.1099/00221287-137-2-227&mimeType=html&fmt=ahah

References

  1. Anwar H., Brown M. R. W., Day A., Weller P. H. 1984; Outer membrane antigens of mucoid Pseudomonas aeruginosa isolated directly from the sputum of a cystic flbrosis patient. FEMS Microbiology Letters 24:235–239
    [Google Scholar]
  2. Benjamin D. C., Berzofsky J. A., East I. J., Gurd F. R. N., Hannum C., Leach S. J., Margoliash E., Michael J. G., Miller A., Prager E. M., Reichlin M., Sercarz E. E., Smith-Gill S. J., Wilson A. C. 1984; The antigenic structure of proteins: a reappraisal. Annual Review of Immunology 2:67–101
    [Google Scholar]
  3. Bolin C. A., Jensen A. E. 1987; Passive immunisation with antibodies against iron-regulated outer membrane proteins protects turkeys from Escherichia coli septicaemia. Infection and Immunity 55:1239–1242
    [Google Scholar]
  4. Brown M. R. W., Anwar H., Lambert P. A. 1984; Evidence that mucoid Pseudomonas aeruginosa in the cystic flbrosis lung grows under iron-restricted conditions. FEMS Microbiology Letters 21:113–117
    [Google Scholar]
  5. Christoffersen A., Cox C. D. 1989; Pyoverdins from Pseudomonas aeruginosa which are effective in iron mobilization from transferrin. Abstracts for the Annual Meeting of the American Society for MicrobiologyD-151
    [Google Scholar]
  6. Cochrane D. M. G., Brown M. R. W., Anwar H., Weller P. H., Lam K., Costerton J. W. 1988; Antibody response to Pseudomonas aeruginosa surface protein antigens in a rat model of chronic lung infection. Journal of Medical Microbiology 27:255–261
    [Google Scholar]
  7. Cornelis P., Moguilevsky N., Jacques J. F., Masson P. L. 1987; Study of the siderophores and receptors in different clinical isolates of Pseudomonas aeruginosa. Antibiotics and Chemotherapy 39:290–306
    [Google Scholar]
  8. Cornelis P., Hohnadel D., Meyer J.-M. 1989; Evidence for different pyoverdine-mediated iron-uptake systems among Pseudomonas aeruginosa strains. Infection and Immunity 57:3491–3497
    [Google Scholar]
  9. Filip C., Fletcher G., Wulff J. L., Earhart C. F. 1973; Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. Journal of Bacteriology 115:717–722
    [Google Scholar]
  10. Gabay J., Schenkman S., Desaymard C., Schwartz M. 1985; Monoclonal antibodies and the structure of bacterial membrane proteins. In Monoclonal Antibodies Against Bacteria249–282 de Macario A. J. L., de Macario C. New York: Academic Press;
    [Google Scholar]
  11. Gilleland H. E., Parker M. G., Matthews J. M., Berg R. D. 1984; Use of a purified outer membrane protein F (porin) preparation of Pseudomonas aeruginosa as a protective vaccine in mice. Infection and Immunity 44:49–54
    [Google Scholar]
  12. Griffiths E. 1987; The iron uptake systems of pathogenic bacteria. Iron and Infection - Molecular, Physiological and Clinical Aspects69–138 Bullen J. J., Griffiths E. Chichester: John Wiley;
    [Google Scholar]
  13. Hackstadt T. 1988; Steric hindrance of antibody binding to surface proteins of Coxiella burnetii by phase I lipopolysaccharide. Infection and Immunity 56:802–807
    [Google Scholar]
  14. Hancock R. E. W., Mutharia L. M., Mouat E. C. A. 1985; Immunotherapeutic potential of monoclonal antibodies against Pseudomonas aeruginosa protein F. European Journal of Clinical Microbiology 4:224–227
    [Google Scholar]
  15. Heckels J. E. 1977; The surface properties of Neisseria gonorrhoeae: isolation of the major components of the outer membrane. Journal of General Microbiology 99:333–341
    [Google Scholar]
  16. Holder I. A., Naglich J. G. 1986; Experimental studies of the pathogenesis of infections due to Pseudomonas aeruginosa: immunization using divalent flagella preparations. Journal of Trauma 26:118–122
    [Google Scholar]
  17. Jarrell K., Kropinski A. M. 1981; Isolation and characterization of the bacteriophage specific for the lipopolysaccharide of rough derivatives of Pseudomonas aeruginosa strain PAO. Journal of Virology 38:529–538
    [Google Scholar]
  18. Kohler G., Milstein C. 1976; Derivation of specific antibody-producing tissue culture and tumour lines by cell fusion. European Journal of Immunology 6:511–519
    [Google Scholar]
  19. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. Lambert P. A., Booth B. R. 1982; Exposure of outer membrane proteins on the surface of Pseudomonas aeruginosa PAO1 revealed by labelling with [125I]lactoperoxidase. FEMS Microbiology Letters 14:43–45
    [Google Scholar]
  21. Leong J., Bitter W., Koster M., Venturi V., Marugg J. D., Weisbeek P. J. 1990; Genetics of iron transport of plant growth-promoting Pseudomonas putida WCS358. (Abstract). International Symposium on Iron Transport, Storage and Metabolism II Austin, TX:23
    [Google Scholar]
  22. Matthews-Greer J. M., Gilleland H. E. 1987; Outer membrane protein F (porin) preparation of Pseudomonas aeruginosa as a protective vaccine against heterologous immunotype strains in a burned mouse model. Journal of Infectious Diseases 155:1282–1291
    [Google Scholar]
  23. Montie T. C., Craven R. C., Holder I. A. 1982; Flagellar preparations from Pseudomonas aeruginosa: isolation and characterization. Infection and Immunity 35:281–288
    [Google Scholar]
  24. Mutharia L. M., Hancock R. E. W. 1983; Surface localization of Pseudomonas aeruginosa outer membrane porin protein F by using monoclonal antibodies. Infection and Immunity 42:1027–1033
    [Google Scholar]
  25. Mutharia L. M., Nicas T. I., Hancock R. E. W. 1982; Outer membrane proteins of Pseudomonas aeruginosa serotype strains. Journal of Infectious Diseases 146:770–779
    [Google Scholar]
  26. Neilands J. B. 1982; Microbial envelope proteins related to iron. Annual Review of Microbiology 36:285–309
    [Google Scholar]
  27. Roberts M., Wooldridge K. G., Gavine H., Iravati Kuswandi S., Williams P. H. 1989; Inhibition of biological activities of the aerobactin receptor protein in rough strains of Escherichia coli by polyclonal antiserum raised against native protein. Journal of General Microbiology 135:2387–2398
    [Google Scholar]
  28. Smith H. 1990; Pathogenicity and the microbe in vivo. Journal of General Microbiology 136:377–383
    [Google Scholar]
  29. Sokol P. A., Woods D. E. 1983; Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa. Infection and Immunity 40:665–669
    [Google Scholar]
  30. Sokol P. A., Woods D. E. 1986a; Characterization of antibody to the ferripyochelin binding protein of Pseudomonas aeruginosa. Infection and Immunity 51:896–900
    [Google Scholar]
  31. Sokol P. A., Woods D. E. 1986b; Monoclonal antibodies to Pseudomonas aeruginosa ferripyochelin binding protein. Infection and Immunity 53:621–627
    [Google Scholar]
  32. Sullivan K. H., Williams R. P. 1982; Use of Iodo-Gen and iodine-125 to label the outer membranes of Neisseria gonorrhoeae. Analytical Biochemistry 34:254–258
    [Google Scholar]
  33. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 764350–4354
    [Google Scholar]
  34. Tsai G. M., Frasch C. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Analytical Biochemistry 119:115–119
    [Google Scholar]
  35. Ward K. H., Anwar H., Brown M. R. W., Wale J., Gowar J. 1988; Antibody response to outer membrane antigens of Pseudomonas aeruginosa in human burn wound infection. Journal of Medical Microbiology 27:179–190
    [Google Scholar]
  36. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-2-227
Loading
/content/journal/micro/10.1099/00221287-137-2-227
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error