pH gradients through colonies of and the surrounding agar Free

Abstract

pH-sensitive microelectrodes, constructed with a tip diameter of about 4 μm, were deployed through 24 h and 48 h colonies of incubated on CYS medium (Casamino acids, yeast extract, salts), with and without glucose. Measurements of pH were used to construct pH profiles through the colony and the surrounding agar. pH gradients could be detected for at least 800 μm into the agar beneath a 24 h colony, and to approximately 10 mm horizontally away from the edge of the colony. In older colonies, the lateral gradient extended for over 20 mm. The pH of the underlying agar was increased by up to 1·45 pH units after 48 h growth without glucose. When colonies were grown with glucose, a significant area of acidification was observed within the colony in addition to a zone of alkalinization present at its periphery. Acidification was thought to be due to the anaerobic fermentation of glucose producing organic acids whilst alkalinization was due to the aerobic oxidation of amino acids releasing ammonia.

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1991-12-01
2024-03-28
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References

  1. Bertl A., Felle H., Bentrup F.-W. 1984; Amine transport in Riccia fluitans Cytoplasmic and vacuolar pH recorded by a pH-sensitive microelectrode. Plant Physiology 76:75–78
    [Google Scholar]
  2. Brown J. P., Huang C. T., Oldershaw M. D., Bibby B. G. 1981; Continuous measurement of plaque pH in vitro . Journal of Dental Research 60:724
    [Google Scholar]
  3. Caldwell P. C. 1954; An investigation of the intracellular pH of crab muscle fibres by means of micro-glass and micro-tungsten electrodes. Journal of Physiology 126:169–180
    [Google Scholar]
  4. Coombs J. P., Wimpenny J. W. T. 1982; Growth of Bacillus cereus in a gel-stabilized nutrient gradient system. Journal of General Microbiology 128:3093–3101
    [Google Scholar]
  5. Cooper A. L., Dean A. C. R., Hinshelwood C. 1968; Factors affecting the growth of bacterial colonies on agar plates. Proceedings of the Royal Society B171175–199
    [Google Scholar]
  6. Hinke J. A. M. 1959; Glass microelectrodes for measuring intracellular activities of sodium and potassium. Nature London: 1841257–1258
    [Google Scholar]
  7. Jones D., Pell P. A., Sneath P. H. A. 1984; Maintenance of bacteria on glass beads at – 60 °C to – 76 °C. Maintenance of Microorganisms35–40 Kirsop B. E., Snell J. J. S. London: Academic Press;
    [Google Scholar]
  8. Jørgensen B. B., Revsbech N. P. 1983; Colorless sulfur bacteria, Beggiatoa spp. and Thiovulum spp., in O2 and H2S microgradients. Applied and Environmental Microbiology 45:1261–1270
    [Google Scholar]
  9. Jørgensen B. B., Revsbech N. P., Cohen Y. 1983; Photosynthesis and structure of benthic microbial mats : microelectrode and SEM studies of four cyanobacterial communities. Limnology and Oceanography 28:1075–1093
    [Google Scholar]
  10. Kamath R. S., Bungay H. R. 1988; Growth of yeast colonies on solid media. Journal of General Microbiology 134:3061–3069
    [Google Scholar]
  11. Nelson D. C., Jørgensen B. B., Revsbech N. P. 1986; Growth pattern and yield of a chemoautotrophic Beggiatoa sp. in oxygen-sulphide microgradients. Applied and Environmental Microbiology 52:225–233
    [Google Scholar]
  12. Peters A. C., Wimpenny J. W. T., Coombs J. P. 1987; Oxygen profiles in, and in the agar beneath, colonies of Bacillus cereus, Staphylococcus albus and Escherichia coli . Journal of General Microbiology 133:1257–1263
    [Google Scholar]
  13. Pirt S. J. 1967; A kinetic study of the mode of growth of surface colonies of bacteria and fungi. Journal of General Microbiology 47:181–197
    [Google Scholar]
  14. Revsbech N. P., Jørgensen B. B. 1986; Microelectrodes : their use in microbial ecology. Advances in Microbial Ecology 9294–357 Marshal K. L. London: Plenum Publishing Corporation;
    [Google Scholar]
  15. Revsbech N. P., Ward D. M. 1984; Microelectrode studies of interstitial water chemistry and photosynthetic activity in a hot spring microbial mat. Applied and Environmental Microbiology 48:270–275
    [Google Scholar]
  16. Rieck V. T., Palumbo S. A., Witter L. D. 1973; Glucose availability and the growth rate of colonies of Pseudomonas fluorescens . Journal of General Microbiology 74:1–8
    [Google Scholar]
  17. Shapiro J. A. 1984; The use of Mudlac transposons as tools for vital staining to visualize clonal and non-clonal patterns of organization in bacterial growth on agar surfaces. Journal of General Microbiology 130:1169–1181
    [Google Scholar]
  18. Shapiro J. A. 1987; Organization of developing Escherichia coli colonies viewed by scanning electron microscopy. Journal of Bacteriology 169:142–156
    [Google Scholar]
  19. Spira W. M., Silverman G. J. 1979; Effects of glucose, pH, and dissolved oxygen tension on Bacillus cereus growth and permeability factor production in batch culture. Applied and Environmental Microbiology 37:109–116
    [Google Scholar]
  20. Thomas R. C. 1978 Ion-sensitive Intracellular Microelectrodes London: Academic Press;
    [Google Scholar]
  21. Walker J. L. 1971; Ion specific liquid ion exchanger microelectrodes. Analytical Chemistry 43:89A–93A
    [Google Scholar]
  22. Weimer P. J. 1984; Control of product formation during glucose fermentation by Bacillus macerans . Journal of General Microbiology 130:103–111
    [Google Scholar]
  23. Wimpenny J. W. T., Coombs J. P. 1983; Penetration of oxygen into bacterial colonies. Journal of General Microbiology 129:1239–1242
    [Google Scholar]
  24. Wimpenny J. W. T., Lewis M. W. A. 1977; The growth and respiration of bacterial colonies. Journal of General Microbiology 103:9–18
    [Google Scholar]
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