1887

Abstract

SUMMARY: Multiple intraperitoneal injections of inactivated Candida albicans cells resulted in the generation of cytotoxic peritoneal cells with phenotypical and functional properties similar to in vitro-generated lymphokine-activated killer (LAK) cells. Using an in vitro [H]glucose uptake assay, -induced LAK-like (CA-LAK) cells exhibited high levels of anti-hyphal activity, the effects being effector to target cell (E:T) ratio- and time-dependent. Maximal levels of anti- activity (approximately 60%) were observed after 4 h and at E:T<300:1. Similar patterns of anti- activity were exerted by -activated natural killer (NK) cells, interleukin-2- (IL-2) generated LAK cells and polymorphonuclear cells. The anti-hyphal activity of CA-LAK cells was enriched by separation on a Percoll gradient, F2 and F3 fractions retaining most of the activity. Experiments using immunodepressed animals demonstrated that the lethality of the .hyphal form is significantly affected by pre-exposure to CA-LAK cells. While control mice receiving alone had a median survival time of 2 d, mice receiving pre-exposed to CA-LAK cells (E:T = 300:1) had a median survival time of 15 d. Overall, the susceptibility of the hyphal form to CA-LAK cells suggests that -induced effectors might play a significant role as a second-line defence mechanism against the hyphal form.

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/content/journal/micro/10.1099/00221287-137-12-2851
1991-12-01
2019-10-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-12-2851
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