1887

Abstract

Multiple intraperitoneal injections of inactivated cells resulted in the generation of cytotoxic peritoneal cells with phenotypical and functional properties similar to -generated lymphokine-activated killer (LAK) cells. Using an [H]glucose uptake assay, . -induced LAK–like (CA-LAK) cells exhibited high levels of anti-hyphal activity, the effects being effector to target cell (E : T) ratio- and time-dependent. Maximal levels of anti- activity (approximately 60%) were observed after 4 h and at E : T ≥ 300:1. Similar patterns of anti- activity were exerted by -activated natural killer (NK) cells, interleukin-2- (IL-2) generated LAK cells and polymorphonuclear cells. The anti-hyphal activity of CA–LAK cells was enriched by separation on a Percoll gradient, F2 and F3 fractions retaining most of the activity. Experiments using immunodepressed animals demonstrated that the lethality of the . hyphal form is significantly affected by pre-exposure to CA–LAK cells. While control mice receiving . alone had a median survival time of 2 d, mice receiving . pre-exposed to CA–LAK cells (E : T = 300:1) had a median survival time of 15 d. Overall, the susceptibility of the . hyphal form to CA–LAK cells suggests that . -induced effectors might play a significant role as a second-line defence mechanism against the . hyphal form.

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1991-12-01
2021-05-12
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