SUMMARY: Multiple intraperitoneal injections of inactivated Candida albicans cells resulted in the generation of cytotoxic peritoneal cells with phenotypical and functional properties similar to in vitro-generated lymphokine-activated killer (LAK) cells. Using an in vitro [H]glucose uptake assay, -induced LAK-like (CA-LAK) cells exhibited high levels of anti-hyphal activity, the effects being effector to target cell (E:T) ratio- and time-dependent. Maximal levels of anti- activity (approximately 60%) were observed after 4 h and at E:T<300:1. Similar patterns of anti- activity were exerted by -activated natural killer (NK) cells, interleukin-2- (IL-2) generated LAK cells and polymorphonuclear cells. The anti-hyphal activity of CA-LAK cells was enriched by separation on a Percoll gradient, F2 and F3 fractions retaining most of the activity. Experiments using immunodepressed animals demonstrated that the lethality of the .hyphal form is significantly affected by pre-exposure to CA-LAK cells. While control mice receiving alone had a median survival time of 2 d, mice receiving pre-exposed to CA-LAK cells (E:T = 300:1) had a median survival time of 15 d. Overall, the susceptibility of the hyphal form to CA-LAK cells suggests that -induced effectors might play a significant role as a second-line defence mechanism against the hyphal form.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error