1887

Abstract

SUMMARY: Cultures of the bacterium were grown in the orbiting Biocosmos 2044 satellite in order to evaluate the effects of the space environment – weightlessness and heavy particle radiation – on growth parameters and energy metabolism, which have previously been reported to be affected, and on induction of the SOS response, which reflects DNA damage to the cell. We found no differences between the flight samples and control ground cultures in the growth yield per gram of carbon, in mean cell mass (from which we deduce that the growth rate was unaltered) or in the level of expression of the SOS response. These observations indicate that free-growing bacterial cells do not expend significant energy fighting gravity and that cosmic radiation within a space capsule does not produce significant levels of DNA damage.

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1991-12-01
2021-10-27
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References

  1. Bradford M. 1976; A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  2. Cogoli A., Tschopp A., Fuchs-Bislin P. 1984; Cell sensitivity to gravity. Science 225:228–230
    [Google Scholar]
  3. D’Ari R., Huisman O. 1983; Novel mechanism of cell division inhibition associated with the SOS response in Escherichia coli . Journal of Bacteriology 156:243–250
    [Google Scholar]
  4. Gmünder F. K., Cogoli A. 1988; Cultivation of single cells in space. Applied Microgravity Technology 1:115–122
    [Google Scholar]
  5. Huisman O., D’Ari R. 1981; An inducible DNA replication-cell division coupling mechanism in E. coli . Nature, London 290:797–799
    [Google Scholar]
  6. Huisman O., D’Ari R., Gottesman S. 1984; Cell division control in Escherichia coli: specific induction of the SOS function SfiA protein is sufficient to block septation. Proceedings of the National Academy of Sciences of the United States of America 814490–4494
    [Google Scholar]
  7. Letaw J. R., Silberberg R., Tsao C. H. 1987; Radiation hazards on space missions. Nature, London 330:709–710
    [Google Scholar]
  8. Limouse M., Manie S., Konstantinova I., Ferrua B., Schaffar L. 1991; Inhibition of phorbol ester-induced cell activation in microgravity. Experimental Cell Research (in the Press)
    [Google Scholar]
  9. Menigmann H. D., Lange M. 1986; Growth and differentiation of Bacillus subtilis under microgravity. Naturwissenschaften 73:415–417
    [Google Scholar]
  10. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Mizusawa S., Court D., Gottesman S. 1983; Transcription of the sulA gene and repression by LexA. Journal of Molecular Biology 171:337–343
    [Google Scholar]
  12. Montgomery P., O’B. Jr, Cook J. E., Reynolds R. C., Paul J. S., Hayflick L., Stock D., Schulz W. W., Kimsey S., Thirolf R. G., Rogers T., Cambell D. 1978; The response of single human cells to zero-gravity. In Vitro 14:165–173
    [Google Scholar]
  13. Nanninga N., Woldringh C. L. 1985; Cell growth, genome duplication, and cell division. In Molecular Cytology of Escherichia coli259–318 Nanninga N. London: Academic Press;
    [Google Scholar]
  14. Quillardet P., Hofnung M. 1984; Induction by UV light of the SOS function sfiA in Escherichia coli strains deficient or proficient in excision repair. Journal of Bacteriology 157:35–38
    [Google Scholar]
  15. Quillardet P., Huisman O., D’Ari R., Hofnung M. 1982; SOS Chromotest, a direct assay of induction of an SOS function in Escherichia coli K-12 to measure genotoxicity. Proceedings of the National Academy of Sciences of the United States of America 795971–5975
    [Google Scholar]
  16. Quillardet P., Frelat G., Nguyen V. D., Hofnung M. 1989; Detection of ionizing radiations with the SOS Chromotest, a bacterial short-term test for genotoxic agents. Mutation Research 216:251–257
    [Google Scholar]
  17. Richoilley G., Tixador R., Gasset G., Templier J., Planel H. 1986; Preliminary results of the “paramecium” experiment. Naturwissenschaften 73:404–406
    [Google Scholar]
  18. Tixador R., Richoilley G., Raffin J., Bost R., Kojarinov V., Lepskye A. 1981; The Cytos biological experiments carried out on the soviet orbital station Salyut 6. Aviation Space and Environmental Medicine 52:485–487
    [Google Scholar]
  19. Walker G. C. 1987; The SOS response of Escherichia coli . In Escherichia coli and Salmonella typhimurium1346–1357 Neidhardt F. C., Ingraham J. L., Low K. B., Magasanik B., Schaechter M., Umbarger H. E. Washington, DC: American Society for Microbiology;
    [Google Scholar]
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