RT Journal Article SR Electronic(1) A1 Wako, Tadayukid A1 Ishikawa, Tatsuo A1 Hashiba, TeruyoshiYR 1991 T1 Unique DNA plasmid pRS64 associated with chromosomal DNAs of the plant pathogenic fungus Rhizoctonia solani JF Microbiology, VO 137 IS 12 SP 2817 OP 2821 DO https://doi.org/10.1099/00221287-137-12-2817 PB Microbiology Society, SN 1465-2080, AB Unique DNA sequences homologous to the linear DNA plasmid pRS64 were investigated in chromosomal DNAs of isolates belonging to anastomosis group 4 (AG-4) of the plant pathogenic fungus Rhizoctonia solani. Chromosome-sized DNAs of isolates RI-64 and 1271 of AG-4 were separated into six bands by orthogonal-field-alternation gel electrophoresis and hybridized to a cloned segment of pRS64. A small chromosome-sized DNA band of approximately 1ยท1 Mb carried the sequences homologous to pRS64 DNA. Sequences homologous to pRS64 were also maintained within the chromosomal DNA of isolate 1271 of AG-4 which does not possess the plasmid. The plasmid showed no homology to the mitochondrial DNA of isolate 1271. The possibility that the linear plasmid pRS64 may act as a transposable genetic element is discussed., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-137-12-2817