1887

Abstract

Each of two affinity isolation methods, the first based on biotinylated porcine transferrin plus streptavidinagarose, and the second on Sepharose-coupled porcine transferrin, followed by SDS-PAGE, allowed the isolation and identification of two potential porcine-transferrin-binding polypeptides (~ 64 kDa and 99 kDa) from total membranes of grown under iron-restricted conditions. Both polypeptides were iron-repressible and were identified as potential receptor candidates as they were not isolated when biotinylated human transferrin was used instead of biotinylated porcine transferrin. The 64 kDa polypeptide was the more easily removed from Sepharose-coupled porcine transferrin and only the 99 kDa polypeptide appeared to be an outer-membrane protein. While these results suggest that the 99 kDa polypeptide represents the porcine transferrin receptor of , and that the 64 kDa polypeptide represents an associated protein serving an accessory role, other interpretations are also possible.

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1991-12-01
2024-04-25
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References

  1. Ala’Aldeen D. A., Davies H. A., Wall R. A., Borriello S. P. 1990; The 70 kilodalton iron regulated protein of Neisseria meningitidis is not the human transferrin receptor. FEMS Microbiology Letters 69:37–42
    [Google Scholar]
  2. Archibald F. S., DeVoe I. W. 1979; Removal of iron from human transferrin by Neisseria meningitidis . FEMS Microbiology Letters 6:159–162
    [Google Scholar]
  3. Archibald F. S., DeVoe I. W. 1980; Iron acquisition by Neisseria meningitidis in vitro. Infection and Immunity 27:322–334
    [Google Scholar]
  4. Black J. R., Dyer D. W., Thompson M. K., Sparling P. F. 1986; Human immune response to iron-repressible outer membrane proteins of Neisseria meningitidis . Infection and Immunity 54:710–713
    [Google Scholar]
  5. Blake M. S., Gotschlich E. C. 1982; Purification and partial characterization of the major outer membrane protein of Neisseria gonorrhoeae . Infection and Immunity 36:277–283
    [Google Scholar]
  6. Brener D., DeVoe I. W., Holbein B. E. 1981; Increased virulence of Neisseria meningitidis after in vitro iron-limited growth at low pH. Infection and Immunity 33:59–66
    [Google Scholar]
  7. Brown M. R. W., Williams P. 1985; The influence of environment on envelope properties affecting survival of bacteria in infections. Annual Review of Microbiology 39:527–556
    [Google Scholar]
  8. Caldwell M., Archibald F. 1987; The effect of the hypoferremic response on iron acquisition by and growth of murine lymphoma cells. Biochemistry and Cell Biology 65:651–657
    [Google Scholar]
  9. Deneer H. G., Potter A. A. 1989a; Iron-repressible outer-membrane proteins of Pasteurella haemolytica . Journal of General Microbiology 135:435–443
    [Google Scholar]
  10. Deneer H. G., Potter A. A. 1989b; Effect of iron restriction on the outer membrane proteins of Actinobacillus (Haemophilus) pleuropneumoniae . Infection and Immunity 57:798–804
    [Google Scholar]
  11. Dyer D. W., West E. P., Sparling P. F. 1987; Effects of serum carrier proteins on the growth of pathogenic neisseriae with heme-bound iron. Infection and Immunity 55:2171–2175
    [Google Scholar]
  12. Frey J., Nicolet J. 1988; Regulation of hemolysin expression in Actinobacillus pleuropneumoniae serotype 1 by Ca2+ . Infection and Immunity 56:2570–2575
    [Google Scholar]
  13. Gonzalez G. C., Caamano D. L., Schryvers A. B. 1990; Identification and characterization of a porcine-specific transferrin receptor in Actinobacillus pleuropneumoniae . Molecular Microbiology 4:1173–1179
    [Google Scholar]
  14. Griffiths E. 1987; The iron-uptake systems of pathogenic bacteria. Iron and Infection ; Molecular, Physiological and Clinical Aspects69–137 Bullen J. J., Griffiths E. New York: John Wiley;
    [Google Scholar]
  15. Griffiths E., Stevenson P., Ray A. 1990; Antigenic and molecular heterogeneity of the transferrin-binding protein of Neisseria meningitidis . FEMS Microbiology Letters 69:31–36
    [Google Scholar]
  16. Harlow E., Lane D. 1988; Silver staining of gels - ammoniacal silver staining. Antibodies: a Laboratory Manual651–652 Cold Spring Harbor NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Herrington D. A., Sparling P. F. 1985; Haemophilus influenzae can use human transferrin as a sole source for required iron. Infection and Immunity 48:248–251
    [Google Scholar]
  18. Lee B. C., Bryan L. E. 1989; Identification and comparative analysis of the lactoferrin and transferrin receptors among clinical isolates of gonococci. Journal of Medical Microbiology 28:199–204
    [Google Scholar]
  19. Lee B. C., Schryvers A. B. 1988; Specificity of the lactoferrin and transferrin receptors in Neisseria gonorrhoeae . Molecular Microbiology 2:827–829
    [Google Scholar]
  20. McKenna W. R., Mickelsen P. A., Sparling P. F., Dyer D. W. 1988; Iron uptake from lactoferrin and transferrin by Neisseria gonorrhoeae . Infection and Immunity 56:785–791
    [Google Scholar]
  21. Martínez J. L., Delgado-Iribarren A., Baquero F. 1990; Mechanisms of iron acquisition and bacterial virulence. FEMS Microbiology Reviews 75:45–56
    [Google Scholar]
  22. Mickelsen P. A., Sparling P. F. 1981; Ability of Neisseria gonorrhoeae, Neisseria meningitidis and commensal Neisseria, species to obtain iron from transferrin and iron compounds. Infection and Immunity 33:555–564
    [Google Scholar]
  23. Mickelsen P. A., Blackman E., Sparling P. F. 1982; Ability of Neisseria gonorrhoeae, Neisseria meningitidis and commensal Neisseria species to obtain iron from lactoferrin. Infection and Immunity 35:915–920
    [Google Scholar]
  24. Mietzner T. A., Luginbuhl G. H., Sandstrom E., Morse S. A. 1984; Identification of an iron-regulated 37,000-Dalton protein in the cell envelope of Neisseria gonorrhoeae . Infection and Immunity 45:410–416
    [Google Scholar]
  25. Morton D. J., Williams P. 1989; Utilization of transferrin-bound iron by Haemophilus species of human and porcine origins. FEMS Microbiology Letters 65:123–128
    [Google Scholar]
  26. Morton D. J., Williams P. 1990; Siderophore-independent acquisition of transferrin-bound iron by Haemophilus influenzae type b. Journal of General Microbiology 136:927–933
    [Google Scholar]
  27. Niven D. F., Donga J., Archibald F. S. 1989; Responses of Haemophilus pleuropneumoniae to iron restriction : changes in the outer membrane protein profile and the removal of iron from porcine transferrin. Molecular Microbiology 3:1083–1089
    [Google Scholar]
  28. Norqvist A., Davies J., Norlander L., Normark S. 1978; The effect of iron starvation on the outer membrane protein composition of Neisseria gonorrhoeae . FEMS Microbiology Letters 4:71–75
    [Google Scholar]
  29. Norrod P., Williams R. P. 1978; Growth of Neisseria gonorrhoeae in media deficient in iron without detection of siderophores. Current Microbiology 1:281–284
    [Google Scholar]
  30. Oakley B. R., Kirsch D. R., Morris N. R. 1980; A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Analytical Biochemistry 105:361–363
    [Google Scholar]
  31. Ogunnariwo J. A., Schryvers A. B. 1990; Iron acquisition in Pasteurella haemolytica : expression and identification of a bovine-specific transferrin receptor. Infection and Immunity 58:2091–2097
    [Google Scholar]
  32. O’Reilly T., Rosendal S., Niven D. F. 1984; Porcine haemophili and actinobacilli : characterization by means of API test strips and possible taxonomic implications. Canadian Journal of Microbiology 30:1229–1238
    [Google Scholar]
  33. Peterson G. L. 1977; A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analytical Biochemistry 83:346–356
    [Google Scholar]
  34. Pidcock K. A., Wooten J. A., Daley B. A., Stull T. L. 1988; Iron acquisition by Haemophilus influenzae . Infection and Immunity 56:721–725
    [Google Scholar]
  35. Pohl S., Bertschinger H. U., Frederiksen W., Mannheim W. 1983; Transfer of Haemophilus pleuropneumoniae and the Pasteurella haemolytica-like organism causing porcine necrotic pleuropneumonia to the genus Actinobacillus (Actinobacillus pleuropneumoniae comb. nov.) on the basis of phenotypic and deoxyribonucleic acid relatedness. International Journal of Systematic Bacteriology 33:510–514
    [Google Scholar]
  36. Schryvers A. B. 1988; Characterization of the human transferrin and lactoferrin receptors in Haemophilus influenzae . Molecular Microbiology 2:467–472
    [Google Scholar]
  37. Schryvers A. B. 1989; Identification of the transferrin- and lactoferrin-binding proteins in Haemophilus influenzae . Journal of Medical Microbiology 29:121–130
    [Google Scholar]
  38. Schryvers A. B., Gonzalez G. C. 1989; Comparison of the abilities of different protein sources of iron to enhance Neisseria meningitidis infection in mice. Infection and Immunity 57:2425–2429
    [Google Scholar]
  39. Schryvers A. B., Gonzalez G. C. 1990; Receptors for transferrin in pathogenic bacteria are specific for the host’s protein. Canadian Journal of Microbiology 36:145–147
    [Google Scholar]
  40. Schryvers A. B., Lee B. C. 1989; Comparative analysis of the transferrin and lactoferrin binding proteins in the family Neisseriaceae . Canadian Journal of Microbiology 35:409–415
    [Google Scholar]
  41. Schryvers A. B., Morris L. J. 1988a; Identification and characterization of the transferrin receptor from Neisseria meningitidis . Molecular Microbiology 2:281–288
    [Google Scholar]
  42. Schryvers A. B., Morris L. J. 1988b; Identification and characterization of the human lactoferrin-binding protein from Neisseria meningitidis . Infection and Immunity 56:1144–1149
    [Google Scholar]
  43. Sebunya T. N. K., Saunders J. R. 1983; Haemophilus pleuropneumoniae infection in swine: a review. Journal of the American Veterinary Medical Association 182:1331–1337
    [Google Scholar]
  44. Simonson C., Brener D., DeVoe I. W. 1982; Expression of a high-affinity mechanism for acquisition of transferrin iron by Neisseria meningitidis . Infection and Immunity 36:107–113
    [Google Scholar]
  45. Tsai J., Dyer D. W., Sparling P. F. 1988; Loss of transferrin receptor activity in Neisseria meningitidis correlates with inability to use transferrin as an iron source. Infection and Immunity 56:3132–3138
    [Google Scholar]
  46. West S. E. H., Sparling P. F. 1985; Response of Neisseria gonoirhoeae to iron limitation: alterations in expression of membrane proteins without apparent siderophore production. Infection and Immunity 47:388–394
    [Google Scholar]
  47. Williams P., Brown M. R. W. 1986; Influence of iron restriction on growth and the expression of outer membrane proteins by Haemophilus influenzae and H. parainfluenzae . FEMS Microbiology Utters 33:153–157
    [Google Scholar]
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