1887

Abstract

Summary: A mutant () of resistant to the polyene antibiotic nystatin was compared with its sensitive, wild-type parent to detect differences in sterol composition using gas chromatography—mass spectrometry. The major sterol in wild-type mycelia, comprising 80% of the total, was ergosterol. The major sterols in mutant mycelia, comprising 86% of the total, were δ814-sterols. It is proposed that the nystatin-resistant strain is unable to synthesize ergosterol because it lacks δ1415-reductase activity as a result of a mutation in the gene.

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/content/journal/micro/10.1099/00221287-137-11-2627
1991-11-01
2021-08-02
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References

  1. Baloch R. I., Mercer E. I. 1987; Inhibition of sterol Δ8→Δ7- isomerase and Δ14-reductase by fenpropimorph, tridemorph and fenpropidin in cell-free enzyme systems from Saccharomyces cerevisiae . Phytochemistry 26:663–668
    [Google Scholar]
  2. Bard M., Woods R. A., Barton D. H. R., Corrie J. E. T., Widdowson D. A. 1977; Sterol mutants of Saccharomyces cerevisiae: chromatographic analyses. Lipids 12:645–654
    [Google Scholar]
  3. Bottema C. K., Parks L. W. 1978; Δ14-Sterol reductase in Saccharomyces cerevisiae . Biochemica et Biophysica Acta 531:301–307
    [Google Scholar]
  4. Fryberg M., Oehlschlager A. C., Unrau A. M. 1974; Sterol biosynthesis in antibiotic-resistant yeast: nystatin. Archives of Biochemistry and Biophysics 160:83–89
    [Google Scholar]
  5. Girling I. J., Hollomon D. W., Kendall S. J., Loeffler R. S. T., Senior I. J. 1988; Effects of fenpropidin on DMI-resistant strains of Erysiphe graminis f. sp. hordei and Rhynchosporium secalis . Proceedings of the British Crop Protection Conference - Pests and Diseases385–390
    [Google Scholar]
  6. Grindle M. 1973; Sterol mutants of Neurospora crassa: their isolation, growth characteristics and resistance to polyene antibiotics. Molecular and General Genetics 120:283–290
    [Google Scholar]
  7. Grindle M. 1974; The efficacy of various mutagens and polyene antibiotics for the induction and isolation of sterol mutants of Neurospora crassa . Molecular and General Genetics 130:81–90
    [Google Scholar]
  8. Grindle M., Farrow R. 1978; Sterol content and enzyme defects of nystatin-resistant mutants of Neurospora crassa . Molecular and General Genetics 165:305–308
    [Google Scholar]
  9. Gollub E. G., Trocha P. K., Liu P. K., Sprinson D. B. 1974; Yeast mutants requiring ergosterol as only lipid supplement. Biochemical and Biophysical Research Communications 56:471–477
    [Google Scholar]
  10. Howell S. A., Moore M. K., Mallet A. I., Noble W. C. 1990; Sterols of fungi responsible for superficial skin and nail infection. Journal of General Microbiology 136:241–247
    [Google Scholar]
  11. Loeffler R. S. T., Hayes A. L. 1990; Sterols of the plant pathogenic fungi Botrytis cinerea and Pyrenophora teres . Phytochemistry 11:3423–3425
    [Google Scholar]
  12. Mercer E. I. 1984; The biosynthesis of ergosterol. Pesticide Science 15:133–155
    [Google Scholar]
  13. Mercer E. I. 1988; The mode of action of morpholines. Sterol Biosynthesis Inhibitors120–150 Berg D., Plempel M. Chichester: Ellis Horwood;
    [Google Scholar]
  14. Osumi T., Taketani S., Katsuki H., Kuhara T., Matsumoto I. 1978; Ergosterol biosynthesis in yeast. Journal of Biochemsitry 83:681–691
    [Google Scholar]
  15. Parks L. W., Bottema C. D. K., Rodrigues R. J., Lewis T. A. 1985; Yeast sterols: yeast mutants as tools for the study of sterol metabolism. Methods in Enzymology 111:333–346
    [Google Scholar]
  16. Rahier A., Benveniste P. 1989; Mass spectral identification of phytosterols. Analysis of Sterols and Other Biologically Significant Sterols223–250 Nes W. D., Parish E. J. London: Academic Press;
    [Google Scholar]
  17. Taton M., Benveniste P., Rahier A. 1989; Microsomal Δ8,14-sterol Δ14-reductase in higher plants. European Journal of Biochemistry 185:605–614
    [Google Scholar]
  18. Trocha P. J., Jasne S. J., Sprinson D. B. 1977; Yeast mutants blocked in removing the methyl group of lanosterol at C-14. Separation of sterols by high-pressure liquid chromatography. Biochemistry 16:4721–4726
    [Google Scholar]
  19. Vogel H. J. 1964; Distribution of lysine pathways among fungi: evolutionary implications. American Naturalist 98:435–446
    [Google Scholar]
  20. Weete J. D. 1989; Structure and function of sterols in fungi. Advances in Lipid Research 23:115–167
    [Google Scholar]
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