1887

Abstract

Using indirect methods based on uptake of [H]tetraphenylphosphonium cation and [C]benzoic acid by cells of the fungus we found that the illumination-induced transient hyperpolarization of the plasma membrane is followed immediately by a rapid temporary decrease in intracellular pH. Hyperpolarization and intracellular acidification were completely suppressed by 150 m-KCI and by the K-ionophore valinomycin. The light-induced acidification of the cytoplasm was not observed in the presence of the cytochrome respiratory chain inhibitors antimycin A and mucidin. Based on these results, we hypothesize that the hyperpolarization of the cells is the consequence of an efflux of K through a light-activated K-channel in the plasma membrane. The loss of positive charge in the cytoplasm caused by this efflux of cations is counterbalanced by H originating from the light-activated mitochondrial respiratory chain.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-11-2605
1991-11-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/11/mic-137-11-2605.html?itemId=/content/journal/micro/10.1099/00221287-137-11-2605&mimeType=html&fmt=ahah

References

  1. Dahlberg K. R., Van Etten J. L. 1982; Physiology and biochemistry of fungal sporulation. Annual Review of Phytopathology 20:281–301
    [Google Scholar]
  2. De la Peña P., Barros F., Gascon S., Ramos S., Lazo P. S. 1982; The electrochemical proton gradient of Saccharomyces . The role of potassium. European Journal of Biochemistry 123:447–453
    [Google Scholar]
  3. Eraso P., Mazon M. J., Gancedo J. M. 1984; Pitfalls in the measurement of membrane potential in yeast cells using tetraphenyl- phosphonium. Biochimica et Biophysica Acta 778:516–520
    [Google Scholar]
  4. Farkaš V., Sulová Z., Lehotský; J. 1985; Effect of light on the concentration of adenine nucleotides in Trichoderma viride . Journal of General Microbiology 131,:317–320
    [Google Scholar]
  5. Gressel J., Rau W. 1983; Photocontrol of fungal development. In Encyclopedia of Plant Physiology 16B603–639 Shropshire W., Mohr H. Berlin, Heidelberg & New York: Springer;
    [Google Scholar]
  6. Grešík M., Kolarova N., Farkaš V. 1988; Membrane potential, ATP and cAMP changes induced by light in Trichoderma viride . Experimental Mycology 12:295–320
    [Google Scholar]
  7. Grešík M., Kolarova N., Farkaš V. 1989; Light stimulated phosphorylation of proteins in cell-free extracts from Trichoderma viride . FEBS Letters 248:185–187
    [Google Scholar]
  8. Krueger B. K. 1989; Towards an understanding of structure and function of ion channels. Federation of American Societies for Experimental Biology Journal 3:1906–1914
    [Google Scholar]
  9. Mitchell P. 1961; Coupling of phosphorylation to electron and hydrogen transfer by a chemiosmotic type of mechanism. Nature London: 191144–148
    [Google Scholar]
  10. Schroeder J. I., Hedrich R. 1989; Involvement of ion channels and active transport in osmoregulation and signalling of higher plant cells. Trends in Biochemical Sciences 14:187–192
    [Google Scholar]
  11. Serrano R. 1985; Evidence for electrogenic proton pumps in fungi and plants. In: Plasma Membrane ATPase of Plants and Fungi38–47 Boca Raton: CRC Press;
    [Google Scholar]
  12. Sulová Z., Hrmová M., Farkaš V. 1990; Photostimulated oxygen uptake in Trichoderma viride . Journal of General Microbiology 136:2287–2290
    [Google Scholar]
  13. Šubík J., Behúň M., Musílek V. 1974; Antibiotic mucidin, a new Antimycin A-like inhibitor of electron transport in rat liver mitochondria. Biochemical and Biophysical Research Communications 57:17–23
    [Google Scholar]
  14. Takeuchi Y., Schmid J., Caldwell J. H., Harold F. M. 1988; Transcellular ion currents and extension of Neurospora crassa hyphae. Journal of Membrane Biology 101:33–41
    [Google Scholar]
  15. Valle E., Bergillos L., Gascon S., Parra F., Ramos S. 1986; Trehalase activation in yeast is mediated by an internal acidification. European Journal of Biochemistry 154:247–251
    [Google Scholar]
  16. Valle E., Bergillos L., Ramos S. 1987; External potassium affects the internal acidification caused by addition of glucose to yeast cells. Journal of General Microbiology 133:535–538
    [Google Scholar]
  17. Van de Mortel J. B. J., Mulders D., Korthout H., Theuvenet A. P. R., Borst-Pauwels G. W. F. H. 1988; Transient hyperpolarization of yeast by glucose and ethanol. Biochimica et Biophysica Acta 936:421–428
    [Google Scholar]
/content/journal/micro/10.1099/00221287-137-11-2605
Loading
/content/journal/micro/10.1099/00221287-137-11-2605
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error