1887

Abstract

To elucidate the repression mechanism of ammonium ions on the biosynthesis of tylosin in NRRL 2702, enzyme activities involved in the metabolism of the aspartate family of amino acids were evaluated in relation to the ammonium ion concentration and tylosin production. It was found that aspartate aminotransferase was essential for both cell growth and tylosin production. However, both threonine dehydratase and valine dehydrogenase were repressed by supplemented ammonium ions at concentrations higher than 50 m. Threonine dehydratase was purified from cell-free extracts by acetone precipitation, ion-exchange chromatography and gel filtration, and its molecular mass was estimated to be 67200 Da. The optimum pH and temperature for threonine dehydratase activity were 7·5 and 25 °C, respectively, and the value for threonine under these optimum conditions was 21 m. The inhibition pattern of ammonium ions on the activity of threonine dehydratase appeared to be a mixed type.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-11-2547
1991-11-01
2021-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/11/mic-137-11-2547.html?itemId=/content/journal/micro/10.1099/00221287-137-11-2547&mimeType=html&fmt=ahah

References

  1. Bergmeyer H. U., Bergmeyer J., Gra M. I. 1983; Phosphoenol pyruvate carboxylase. Methods of Enzymatic Analysis 2275–276 Bergmeyer H. U., Gra M., Weinham I. Verlag Chemie;
    [Google Scholar]
  2. Bode R., Birnbaum D. 1986; Threonine dehydratase activity from several yeast species is activated and affected by phosphate. FEMS Microbiology Letters 37:189–192
    [Google Scholar]
  3. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  4. Corcoran J. W. 1974; Lipid and macrolide lactone biosynthesis in Streptomyces erythreus . Developments in Industrial Microbiology 15:83–100
    [Google Scholar]
  5. Datta P. 1971; Threonine deaminase - biosynthetic (Rhodopseudomonas sphaeroides). Methods in Enzymology 17B:566–571
    [Google Scholar]
  6. Hatfield G. W., Umbarger H. E. 1971; Threonine deaminase -degradative (Bacillus subtilis). Methods in Enzymology 17B:571–575
    [Google Scholar]
  7. Rang H. A., Lee K. J. 1987a; Regulation of cell growth and tylosin biosynthesis through flux control of metabolite intermediate in Streptomyces fradiae . Korean Journal of Microbiology 25:189–197
    [Google Scholar]
  8. Rang H. A., Lee K. J. 1987b; Regulation of tylosin biosynthesis by cell growth rate in Streptomyces fradiae . Korean Journal of Microbiology 25:353–359
    [Google Scholar]
  9. Kennedy J. H. 1978; Liquid chromatographic analysis of fermentation broths : cephalosporin C and tylosin. Journal of Chromatographic Science 16:492–495
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature London: 227680–685
    [Google Scholar]
  11. Lee J. H., Lee K. J. 1987; Effect of growth rate on the tylosin biosynthesis in Streptomyces fradiae . Korean Journal of Applied Microbiology and Bioengineering 15:34–38
    [Google Scholar]
  12. Morin R. B., Gorman M. 1964; The partial structure of tylosin, a macrolide antibiotic. Tetrahedron Letters 14:2339–2345
    [Google Scholar]
  13. Morrissey J. H. 1981; Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Analytical Biochemistry 117:307–310
    [Google Scholar]
  14. Nakazawa A. 1971; Threonine deaminase - degradative (Clostridium tetanomorphum). Methods in Enzymology 17B:571–575
    [Google Scholar]
  15. Navarrete R. M., Vara J. A., Hutchinson C. R. 1990; Purification of an inducible l-valine dehydrogenase of Streptomyces coelicolor A3(2). Journal of General Microbiology 136:273–281
    [Google Scholar]
  16. Omura S., Takeshima A., Nakagawa A., Priiou F., Lukacs G. 1977; Studies on the biosynthesis of 16-membered macrolide antibiotics using carbon-13 nuclear magnetic resonance spectroscopy. Biochemistry 16:2860–2866
    [Google Scholar]
  17. Omura S., Tsuzui K., Tanaka Y., Sakakibara H., Mamada H., Masuma R. 1983; Ammonium ion suppresses the biosynthesis of tylosin aglycone by interference with valine catabolism in Streptomyces fradiae . Journal of Antibiotics 36:1792–1794
    [Google Scholar]
  18. Omura S., Taki A., Matsuda K., Tanaka A. 1984; Effect of ammonium ion, inorganic phosphate and amino acids on the biosynthesis of protylonolide, a precursor of tylosin aglycone. Journal of Antibiotics 37:1362–1369
    [Google Scholar]
  19. Seno E. T., Pieper R. L., Huber F. M. 1977; Terminal stages in the biosynthesis of tylosin. Actimicrobial Agents and Chemotherapy 11:455–461
    [Google Scholar]
  20. Shizuta Y., Tokushige M. 1971; Threonine deaminase (degradative) (Escherichia coli). Methods in Enzymology 17B:575–580
    [Google Scholar]
  21. Taras M. J., Arnold E. F., Hoak R. D., Rand M. C. 1981; Phenate method. Standard Methods for the Examination of Water and Wastewater350–352 Taras M. J., Arnold E. C., Hoak R. D., Rand M. C. Washington, DC: American Public Health Association;
    [Google Scholar]
  22. Vancura A., Rezanka T., Marsalek K., Vancular I., Kristan V., Basarova G. 1987; Effect of ammonium ions on the composition of fatty acids in Streptomyces fradiae producer of tylosin. FEMS Microbiology Letters 48:357–358
    [Google Scholar]
  23. Vancura A., Rezanka T., Marsalek J., Melzoch K., Basarova G., Kristan V. 1988; Metabolism of l-threonine and fatty acids and tylosin biosynthesis in Streptomyces fradiae . FEMS Microbiology Letters 49:411–415
    [Google Scholar]
  24. Vancurova A. I., Volc J., Fussey S. P. M., Flieger M., Neuzil J., Marsalek J., Behal V. 1988; Valine dehydrogenase from Streptomyces fradiae: purification and properties. Journal of General Microbiology 134:3213–3219
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-11-2547
Loading
/content/journal/micro/10.1099/00221287-137-11-2547
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error