The spontaneous and recessive mutation in the basidiomycete suppresses the formation of aerial hyphae in the monokaryon and, if present as a double dose, the formation of both aerial hyphae and fruit-bodies in the dikaryon. In the monokaryon, the mutation prevents accumulation of mRNA of the gene, and in the dikaryon it also prevents the accumulation of fruiting-specific mRNAs, including mRNAs of the and genes, which are homologous to the gene. These three genes code for hydrophobins, a family of small hydrophobic cysteine-rich proteins. In the monokaryon, the only detectable change in synthesized proteins is the disappearance of an abundant protein of apparent = 28 K from the culture medium and from the cell walls. Protein sequencing shows that this is the product of the gene. The Sc3 hydrophobin is present in the walls of aerial hyphae as a hot-SDS-insoluble complex. Submerged hyphae excrete large amounts of the hydrophobin into the medium.


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