A 3·8 kb PstI fragment of Mycobacterium tuberculosis was cloned in a recA-deleted Escherichia coli by selecting transformants with increased EMS resistance. The cloned fragment restored homologous recombination in Hfr crosses and conferred resistance to long wave (302 nm) but not short wave (254 nm) UV light. E. coli containing the 3·8 kb PstI fragment produced a 38–40 kDa protein which cross-reacted with antibodies raised against the E. coli RecA protein. The cloned DNA thus probably encodes a RecA homologue.
BaltzR. H.,
StonesiferJ.1985; Adaptive response and enhancement of N-methyl-N′-nitro-N-nitrosoguanidine mutagenesis by chloramphenicol in Streptomyces fradiae. Journal of Bacteriology 164:944–946
BaltzR. H.1987; Mechanism of mutation and DNA repair in Streptomyces fradiae. Proceedings of the Fifth International Symposium for the Genetics of Industrial Microorganisms, Part A85–94AlacevicM.,
HranueliD.,
TomanZ.
Pliva: Zagreb;
BibbM. J.,
CohenS. N.1982; Gene expression in Streptomyces: construction and application of promoter-probe vectors in Streptomyces lividans. Molecular & General Genetics 187:265–277
BolivarF.,
RodriguezR. L.,
GreeneP. J.,
BetlachH. L.,
HeyneckerH. L.,
BoyerH. W.,
CrosaJ. H.,
FalkowS.1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
BoyerH. W.,
Roulland-DussioxD.1969; A complementation analysis of the restriction and modification of DNA in Escherichia coli. Journal of Molecular Biology 41:459–472
CasadabanM. J.,
CohenS. N.1980; Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. Journal of Molecular Biology 138:179–207
DutreixM.,
MoreauP. L.,
BailoneA.,
GalibertF.,
BattistaJ. R.,
WalkerG. C.,
DevoretR.1989; New recA mutations that dissociate the various RecA protein activities in Escherichia coli. provide evidence for an additional role for RecA protein UV mutagenesis. Journal of Bacteriology 171:2415–2423
EnnisD. G.,
OssannaN.,
MountD. W.1989; Genetic separation of Escherichia coli rec A functions for SOS mutagenesis and repressor cleavage. Journal of Bacteriology 171:2533–2541
GoodmanH. J. K.,
ParkerJ. R.,
SouthernJ. A.,
WoodsD. R.1987; Cloning and expression in Escherichia coli of a recA-like gene from Bacteroides fragilis. Gene 58:265–271
HaroldR. J.,
HopwoodD. A.1970a; Ultraviolet-sensitive mutants of Streptomyces coelicolor I. Phenotypic characterisation. Mutational Research 10:427–438
HaroldR. J.,
HopwoodD. A.1972; A rapid method for complementation testing of ultraviolet sensitive (uvs) mutants of Streptomyces coelicolor. Mutation Research 16:27–34
Howard-FlandersP.,
BoyceR. P.1966; DNA repair and genetic recombination: studies on mutants of Escherichia coli defective in these processes. Radiation Research (supplement) 6:156–184
HromicA.,
KirbyR.1987; Isolation study of three mutants of Streptomyces cattleya affecting DNA repair and genetic instability. FEMS Microbiology Letters 60:150–155
JohnsonD. A.,
GautschJ. W.,
SportsmanJ. R.,
ElderJ. H.1984; Improved technique using non fat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Gene Analysis Techniques 1:3–8
KokjohnT. A.,
MillerR. V.1985; Molecular cloning and characterisation of the recA gene of Pseudomonas aeruginosa PAO. Journal of Bacteriology 163:568–572
LittleJ. W.,
EdmistonS. H.,
PacelliL. Z.,
MountD. W.1980; Cleavage of the Escherichia coli lexA protein by the rec A protease. Proceedings of the National Academy of Sciences of the United States of America773225–3229
MarreroR.,
YasbinR. E.1988; Cloning of the Bacillus subtilis recA+ gene and functional expression of rec A+ in Bsubtilis. Journal of Bacteriology 170:335–344
MatsushimaP.,
BaltzT. H.1987; RecA gene of Escherichia coli complements defects in DNA repair and mutagenesis in Streptomyces fradiae JSE (mcr-6). Journal of Bacteriology 169:4834–4836
McEnteeK.,
WeinstockG. M.,
LehmanI. R.1979; Initiation of general recombination catalysed in vitro by the RecA protein of Escherichia coli. Proceedings of the National Academy ofSciences ofthe United States of America762615–2619
NohmiT.,
BattistaJ. R.,
DodsonL. A.,
WalkerG. C.1988; RecA mediated cleavage activities UmuD for mutagenesis: mechanistic relationship between transcriptional derepression and post- translational activation. Proceedings of the National Academy of Sciences of the United States of America851816–1820
OwttrimG. W.,
ColemanJ. R.1989; Regulation of expression and nucleotide sequence of the Anabaena variabilis rec A gene. Journal of Bacteriology 171:5713–5719
ParrishJ. A.,
AndersonR. R.,
UrbachF.,
PittsD.1981; Effects of ultraviolet radiation on microorganisms and animal cells. Biological Effects of Ultraviolet Radiation with Emphasis on Human Responses to Longwave Ultraviolet, Chptr 5 New York and London: Plenum Press;
PetersonK. R.,
MountD. W.1987; Differential repression of SOS genes by unstable lexA41(tsl-l) protein causes a ‘split- phenotype’ in Escherichia coli K-12. Journal of Molecular Biology 193:27–40
RamesarR. S.,
WoodsD. R.,
RawlingsD. E.1988; Cloning and expression in Escherichia coli of a recA-like gene from the acidophilic autotroph Thiobacillus ferrooxidans. Journal of General Microbiology 134:1141–1146
RamesarR. S.,
AbrattV.,
WoodsD. R.,
RawlingsD. E.1989; Nucleotide sequence and expression of a cloned Thiobacillus ferrooxidans rec A gene in E. coli. Gene 78:1–8
SancarA.,
StachelekC.,
KoningsbergW.,
RuppW. D.1980; Sequences of the recA gene and protein. Proceedings of the National Academy of Sciences of the United State of America772611–2615
SetlowJ. K.,
SpikesD.,
GriffinK.1988; Characterization of the reel gene of Haemophilus influenzae and behavior of the gene in Escherichia coli. Journal of Bacteriology 170:3876–3881
ShinagawaH.,
IwasakiH.,
KatoT.,
NakataA.1988; RecA protein dependent cleavage of UmuD protein and SOS mutagenesis. Proceedings of the National Academy of Sciences of the United States of America841806–1810
TholeJ. E. R.,
DauwerseH. G.,
DasP. K.,
GroothuisD. G.,
SchoulsL. M.,
Von EmbdenJ. D. A.1985; Cloning of Mycobacterium bovis BCG DNA and expression of antigens in Escherichia coli. Infection and Immunity 50:800–806
TowbinH.,
StaehelinT.,
GordonJ.1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America764350–4354
TessmanE. S.,
PetersonP. K.1985; Isolation of protease- proficient, recombinase-deficient rec A mutants of E. coli K-12. Journal of Bacteriology 163:688–695
UsdinK.,
GertschK.,
KirbyR.1985; The loss of a large DNA fragment is associated with an aerial mycelium negative (Am−) phenotype of Streptomyces cattleya. Journal of General Microbiology 131:979–981
VieiraJ.,
MessingJ.1982; The pUC plasmids, an M13mp7 derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
WalkerG. C.,
ElledgeS. J.,
KenyonC. J.,
KruegerJ. H.,
PerryK. L.1982; Mutagenesis and other responses induced by DNA damage in Escherichia coli. Biochemie 64:607–610
WalkerG. C.,
ShevellD. E.,
BattistaJ. R.1987; Mutagenesis and cellular responses to DNA damage. In Proceedings of the Fifth International Symposium on the Genetics of Industrial Microorganisms, Part A65–71AlvacevicM.,
HranueliD.,
TomanZ.
Pliva: Zagreb;
WillisD. K.,
UhlinB. E.,
AminiK. S.,
ClarkA. J.1981; Physical mapping of the srlrec A region of Escherichia coli: analysis of the Tn10 generated insertions and deletions. Molecular & General Genetics 183:497–504
de WitD.,
SteynL.,
ShoemakerS.,
SoginM.1990; Direct detection of Mycobacterium tuberculosis in clinical specimens by DNA amplification. Journal of Clinical Microbiology 28:2437–2441
ZabeauM.,
StanleyK. K.1982; Enhanced expression of cro-B- galactosidase fusion proteins under the control of the Pr promoter of bacteriophage lambda. EMBO Journal 1:1217–1224