RT Journal Article SR Electronic(1) A1 Guffanti, Arthur A. A1 Hicks, David B.YR 1991 T1 Molar growth yields and bioenergetic parameters of extremely alkaliphilic Bacillus species in batch cultures, and growth in a chemostat at pH 10·5 JF Microbiology, VO 137 IS 10 SP 2375 OP 2379 DO https://doi.org/10.1099/00221287-137-10-2375 PB Microbiology Society, SN 1465-2080, AB Alkaliphilic Bacillus species that grow at pH 10·5 must cope with a low protonmotive force (– 50 mV) due to a reversed transmembrane pH gradient at least 2 pH units more acid inside. Here we demonstrate that strictly alkaliphilic B. firmus RAB and two strains of B. alcalophilus (ATCC 27467 and DSM 485) grow exponentially in batch cultures with a doubling time of less than 1 h in 100 mm buffered medium, while the actual medium pH remains above 10·2. The ATCC strain continued to grow rapidly for at least 7 h, but the growth rate of the DSM strain declined dramatically after 3 h. However, both the B. alcalophilus strains, B. firmus RAB and facultatively alkaliphilic B. firmus OF4 were readily maintained for at least 24 h between pH 10·4 and 10·6 in a chemostat where nutrients were constantly replenished. A critical nutrient may be limiting in batch cultures of the DSM strain of B. alcalophilus. The facultative alkaliphile grew equally well in batch cultures at an initial pH of 7·5 or 10·5. Its molar growth yield (23 mg dry wt mmol−1) on malate (Y mal) was the same at the two pH values and was comparable to Y mal for B. subtilis grown at neutral pH. B. firmus RAB and B. alcalophilus ATCC 27467 grown at pH 10·5 also showed Y mal values at least as high as the neutralphile, indicating efficient use of the energy source even at low protonmotive force. Moreover, the phosphorylation potential of B. firmus OF4 grown at pH 7·5 (45·2 kJ mol−1)or pH 10·5 (46 kJ mol−1) was in a conventional range for bacteria., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-137-10-2375