Phages JHJ-2 and JHJ-3 were isolated from Saccharopolyspora hirsuta 367 UC 8106 following induction with mitomycin C and amplified on S. hirsuta NRRL B-5792. Their properties were compared with those of phage JHJ-1, isolated previously from S. hirsuta 367 NRRL 12045. The DNA restriction patterns appeared to be identical. One-step growth experiments showed no differences between the replication cycles. Burst sizes ranged from 100 to 110 p.f.u. per cell. However, the three phages showed some differences in their behaviour in different hosts. The host range of phage JHJ-1, on non-lysogenic strains, was emended to include all of the Saccharopolyspora strains tested; the host range of phage JHJ-2 was shown to be identical to JHJ-1. Phage JHJ-3 did not form detectable plaques on strains of S. rectivirgula or S. erythraea except S. erythraea NRRL 2359. Neither phage JHJ-2 nor JHJ-3 formed plaques on any lysogenic strains, while JHJ-1 formed plaques on all such strains except S. hirsuta 367 UC 8106. Phage JHJ-3 was characterized as a temperate bacteriophage because it formed turbid, self-limiting plaques and lysogenized S. hirsuta NRRL B-5792. It was spontaneously released from UC 8106. Both JHJ-1 and JHJ-2 formed clear and invasive (Inv+ phenotype: the property to grow on old mycelium) plaques on some Saccharopolyspora strains but clear and self-limiting plaques on others. Thus, the expression of the Inv+ phenotype encoded by JHJ-1 and JHJ-2 appears to be modulated by the host cell.
BrzeziǹskiR.,
SurmaczE.,
KutnerM.,
PiekarowiczA.1986; Restriction mapping and close relationship of the DNA of Streptomyces erythraeus phages 121 and SE-5. Journal of General Microbiology 132:2937–2943
HaketJ.Jr,
DesmaraisD.,
MemndateK.,
DéryC. V.1990; Saccharopolyspora hirsuta strain 367 releases JHJ-1, a bacteriophage capable of propagation on old mycelium. Journal of General Microbiology 136:573–579
JohnsonA. D.,
PoteeteA. R.,
LauerG.,
SauerR. T.,
AckersG. K.,
PasthneM.1981; Lambda repressor and cro – components of an efficient molecular switch. Nature London: 294:217–223
KatzL.,
ChiangS.-J. D.,
TuanJ. S.,
ZablenL. B.1988; Characterization of bacteriophage ϕC69 of Saccharopolyspora erythraea and demonstration of heterologous actinophage propagation by transfection of Streptomyces and Saccharopolyspora
. Journal of General Microbiology 134:1765–1771
Korn-WendischF.,
KempfA.,
GrundE.,
KroppenstedtR. M.,
KutznerH. J.1989; Transfer of Faenia rectivirgula Lacey and Goodfellow 1975, elevation of Saccharopolyspora hirsuta subsp. taberi Labeda 1987 to species level, and emended description of the genus Saccharopolyspora
. International Journal of Systematic Bacteriology 39:430–441
KurupV. P.1984; Thermophilic actinomycetes: their role in hypersensitivity pneumonitis. Biological, Biochemical and Biomedical Aspects of Actinomycetes145–159Ortiz-OrtizL.,
BojalilL. F.,
YakoleffV.
London: Academic Press;
LabedaD. P.1987; Transfer of the type strain of Streptomyces erythraeus (Waksman, 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov. and designation of a neotype strain for Streptomyces erythraeus
. International Journal of Systematic Bacteriology 37:19–22
LaceyJ.,
GoodfellowM.1975; A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et sp. nov. Journal of General Microbiology 88:75–85
PaulusT. J.,
TuanJ. S.,
LuebkeV. E.,
MaineG. T.,
DewittJ. P.,
KatzL.1990; Mutation and cloning of eryG, the structural gene for erythromycin O-methyltransferase from Saccharopolyspora erythraea and expression of eryG in Escherichia coli
. Journal of Bacteriology 172:2541–2546
PepysJ.,
JenkinsP. A.,
FestensteinG. N.,
GregoryP. H.,
LaceyM. E.,
SkinnerS. A.1963; Farmer’s lung: thermophilic actinomycetes as a source of “farmer’s lung hay” antigen. Lancet ii:607–611
SchneiderJ.,
KutznerH. J.1989a; Distribution of homologies among the genomes of several actinophages of Faenia and Saccharopolyspora as determined by DNA hybridization. Intervirology 30:237–240
SchneiderJ.,
KutznerH. J.1989b; Distribution of modules among the central regions of the genomes of several actinophages of Faenia and Saccharopolyspora
. Journal of General Microbiology 135:1671–1678
SchneiderJ.,
GarciaI. A.,
KutznerH. J.1987; Characterization of a family of temperate actinophages of Faenia rectivirgula
. Journal of General Microbiology 133:2263–2268
SmorawinskaM.,
DenisF.,
DéryC. V.,
MagnyP.,
BrzezinskiR.1988; Characterization of SE-3, a virulent bacteriophage of Saccharopolyspora erythraea
. Journal of General Microbiology 134:1773–1778