Phages JHJ-2 and JHJ-3 were isolated from 367 UC 8106 following induction with mitomycin C and amplified on NRRL B-5792. Their properties were compared with those of phage JHJ-1, isolated previously from 367 NRRL 12045. The DNA restriction patterns appeared to be identical. One-step growth experiments showed no differences between the replication cycles. Burst sizes ranged from 100 to 110 p.f.u. per cell. However, the three phages showed some differences in their behaviour in different hosts. The host range of phage JHJ-1, on non-lysogenic strains, was emended to include all of the strains tested; the host range of phage JHJ-2 was shown to be identical to JHJ-1. Phage JHJ-3 did not form detectable plaques on strains of or except NRRL 2359. Neither phage JHJ-2 nor JHJ-3 formed plaques on any lysogenic strains, while JHJ-1 formed plaques on all such strains except 367 UC 8106. Phage JHJ-3 was characterized as a temperate bacteriophage because it formed turbid, self-limiting plaques and lysogenized NRRL B-5792. It was spontaneously released from UC 8106. Both JHJ-1 and JHJ-2 formed clear and invasive (Inv phenotype: the property to grow on old mycelium) plaques on some strains but clear and self-limiting plaques on others. Thus, the expression of the Inv phenotype encoded by JHJ-1 and JHJ-2 appears to be modulated by the host cell.


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