Cloning and characterization of the ColE7 plasmid Free

Abstract

Summary: The 2·6 kb ColE7-K317 plasmid was mapped and the DNA fragments of the colicin E7 operon subcloned into pUC18 and pUC19. The size of the functional colicin E7 operon deduced by subcloning was 2·3 kb. The colicin E7 gene product was purified by carboxymethylcellulose chromatography. Both colicin E7 and E9 were demonstrated to exhibit a non-specific DNAase-type activity by biological assay. The molecular mass of colicin E7 was 61 kDa, as determined by SDS-PAGE. From DNA sequence data, the estimated sizes of the E7 immunity protein and the E7 lysis protein were 9926 Da and 4847 Da, respectively. Comparison of restriction maps and DNA sequence data suggests that ColE7 and ColE2 are more closely related than other E colicin plasmids.

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/content/journal/micro/10.1099/00221287-137-1-91
1991-01-01
2024-03-28
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References

  1. Bowman C. M., Dahlberg J. E., Ikemura T., Konisky J., Nomura M. 1971; Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo . Proceedings of the National Academy of Sciences of the United States of America 68964–968
    [Google Scholar]
  2. Birnboim H. C. 1983; A rapid alkaline extraction procedure for screening plasmid DNA. Methods in Enzymology 100B:243–255
    [Google Scholar]
  3. Chak K.-F., James R. 1984; Localization and characterization of a gene on the ColE3-CA38 plasmid that confers immunity to colicin E8. Journal of General Microbiology 130:701–710
    [Google Scholar]
  4. Chak K.-F., James R. 1985; Analysis of the promoters for the two immunity genes present in the ColE3-CA38 plasmid using two new promoter probe vectors. Nucleic Acids Research 13:2519–2531
    [Google Scholar]
  5. Chak K.-F., James R. 1986; Characterization of the ColE9-J plasmid and analysis of its genetic organization. Journal of General Microbiology 132:61–71
    [Google Scholar]
  6. Chen E. Y., Seeburg P. H. 1985; Supercoil sequencing: fast and simple method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  7. Cole S. T., Saint-Joanis B., Pugsley A. P. 1985; Molecular characterization of the colicin E2 operon and identification of its products. Molecular and General Genetics 198:465–472
    [Google Scholar]
  8. Cooper P. C., James R. 1984; Two new E colicins, E8 and E9, produced by a strain of Escherichia coli . Journal of General Microbiology 130:209–215
    [Google Scholar]
  9. Curtis M. D., James R., Coddington A. 1989; An evolutionary relationship between the ColE5-099 and ColE9-J plasmids revealed by nucleotide sequencing. Journal of General Microbiology 135:2783–2788
    [Google Scholar]
  10. Dretzen G., Bellard M., Sassone-Corsi P., CHAMBON P. 1981; A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Analytical Biochemistry 112:295–298
    [Google Scholar]
  11. Fredericq P. 1949; Sur la resistance croisée entre colicine E et bacteriophage II. Comptes Rendus des Séances de la Société de biologie 143:1011–1013
    [Google Scholar]
  12. Herschman H. R., Helinski D. R. 1967; Comparative study of the events associated with colicin induction. Journal of Bacteriology 94:691–699
    [Google Scholar]
  13. Jakes K., Zinder N. D., Boon T. 1974; Purification and properties of colicin E3 immunity protein. Journal of Biological Chemistry 249:438–444
    [Google Scholar]
  14. James R., Jarvis M., Barker D. F. 1987; Nucleotide sequence of the immunity and lysis region of the ColE9-J plasmid. Journal of General Microbiology 133:1553–1562
    [Google Scholar]
  15. Konisky J. 1982; Colicins and other bacteriocins with established modes of action. Annual Review of Microbiology 36:125–144
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning - a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Masaki H., Ohta T. 1985; Colicin E3 and its immunity genes. Journal of Molecular Biology 182:217–227
    [Google Scholar]
  18. Masaki H., Toba M., Ohta T. 1985; Structure and expression of the ColE2-P9 immunity gene. Nucleic Acids Research 13:1623–1635
    [Google Scholar]
  19. McKenny K., Schimatake H., Court D., Scheissner V., Brady C., Rosenberg. 1981; A system to study promoter and terminator signals recognised by Escherichia coli RNA polymerase. Gene Amplification and Analysis 2383–415 Chirikjian J. G., Papas T. Amsterdam: Elsevier-North Holland;
    [Google Scholar]
  20. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Pugsley A. P., Oudega B. 1987; Methods for studying colicins and their plasmids. Plasmids - a Practical Approach105–161 Hardy K. G. Oxford & Washington, DC: IRL Press;
    [Google Scholar]
  22. Reeves P. 1965; The adsorption and kinetics of killing by colicin CA 42-E2. Australian Journal of Experimental Biology and Medical Science 43:191–200
    [Google Scholar]
  23. Rosenberg M., Court D. 1979; Regulatory sequences involved in the promotion and termination of RNA transcription. Annual Review of Genetics 13:319–353
    [Google Scholar]
  24. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 745463–5467
    [Google Scholar]
  25. Schaller K., Momura M. 1976; Colicin E2 is a DNA endonuclease. Proceedings of the National Academy of Sciences of the United States of America 733989–3993
    [Google Scholar]
  26. Tabor S., Richardson C. C. 1987; DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences of the United States of America 844767–4771
    [Google Scholar]
  27. Toba M., Masaki H., Ohta T. 1988; Colicin E8, a DNase which indicates an evolutionary relationship between colicins E2 and E3. Journal of Bacteriology 170:3237–3242
    [Google Scholar]
  28. Tokuda H., Konisky J. 1979; Effect of colicins la and El on ion permeability of liposomes. Proceedings of the National Academy of Sciences of the United States of America 766167–6171
    [Google Scholar]
  29. Uchimura T., Lau P. 1987; Nucleotide sequences from the colicin E8 operon : homology with plasmid ColE2-P9. Molecular and General Genetics 209:489–493
    [Google Scholar]
  30. Watson R., Rowsome W., Tsao J., Visentin L. P. 1981; Identification and characterization of Col plasmids from classical colicin E-producing strains. Journal of Bacteriology 147:569–577
    [Google Scholar]
  31. Watson R., Vernet T., Visentin L. P. 1985; Relationships of the Col plasmids E2, E3, E4, E6 and E7 : restriction mapping and colicin gene fusions. Plasmid 13:205–210
    [Google Scholar]
  32. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains : nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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