1887

Abstract

Summary: The enzyme profiles of 20 oral and non-oral strains were investigated using an API ZYM Complete Research kit. The test included 10 2-naphthyl derivatives of fatty acids, 20 -nitrophenol derivatives of carbohydrates and 60 2-naphthylamide derivatives of amino acids and peptides. The oral species investigated were and . The non-oral species examined were and intestinal spirochaetes of human and chicken origin. Esterase activities on C to C fatty acids were common among different Treponema species. Glycosidase activities were infrequently observed in and Reiter strain. Arabinosidase, lactosidase and xylosidase activity was observed in the T. strains but α-L-fucosidase activity was found only in and . More exo- and endo-peptidase activities were found in than in other species. The enteropathogenic isolates had a very low proteolytic profile. Dipeptidyl prolyl amidase activity was observed in all species except in the Reiter strain and the avian intestinal spirochaetes. The enzyme profiles did not discriminate between oral and non-oral species.

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1991-01-01
2021-05-10
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References

  1. Carlsson J., Nilsson T., Lundquist G. 1986; Effect of proteinases from black pigmented Bacteroides on human plasma proteins. The Borderland between Caries and Periodontal Disease III155–163 Lehner T., Cimasoni G. Geneva: Editions Médicines et Hygiène;
    [Google Scholar]
  2. Davelaar F. G., Smit H. F., Houvin Hougen K., Dwars R. M., van den Valk P. C. 1986; Infectious typhlitis in chickens caused by spirochetes. Avian Pathology 15:247–258
    [Google Scholar]
  3. Fiehn N. E. 1986; Enzyme profiles from eight small-sized oral spirochetes. Scandinavian Journal of Dental Research 94:132–140
    [Google Scholar]
  4. Fukasawa K., Hiraoka B. Y., Fukasawa K. M., Harada M. 1982; Arylamidase activities specific for proline, tyrosine and basic amino acid residues in some oral bacteria. Journal of Dental Research 61:818–820
    [Google Scholar]
  5. Fukumoto Y., Okuda K., Kato T., Takazoe I. 1987; Taxonomic study of spirochetes isolated from human periodontal lesions. Oral Microbiology and Immunology 2:82–87
    [Google Scholar]
  6. Gibbons R. J., MacDonald J. B. 1960; Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus . Journal of Bacteriology 80:164–170
    [Google Scholar]
  7. Grenier D., Uitto M. J., McBride B. C. 1990; Cellular location of a Treponema denticola chymotrypsin like protease and importance of the protease in migration through the basement membrane. Infection and Immunity 58:347–351
    [Google Scholar]
  8. Harris D. L., Glock R. D., Kinyon J. M. 1976; Intestinal treponematoses. The Biology of Parasitic Spirochetes277–294 Johnson R. C. New York: Academic Press;
    [Google Scholar]
  9. Hunter D., Wood T. 1979; An evaluation of the API-ZYM system as a means of classifying spirochaetes associated with swine dysentery. Veterinary Record 104:383–384
    [Google Scholar]
  10. Kennedy G. A., Strafuss A. C., Schoenweis D. A. 1973; Scanning electronic microscopic observations on swine dysentery. Journal of American Veterinary Association 163:53–55
    [Google Scholar]
  11. Laughton B. E., Syed S. A., Loesche W. J. 1981; API-ZYM system for identification of Bacteroides spp., Capnocytophaga spp. and spirochetes of oral origin. Journal of Clinical Microbiology 15:97–102
    [Google Scholar]
  12. Listgarten M. A. 1965; Electron microscopic observations on the bacterial flora of acute nectrotizing ulcerative gingivitis. Journal of Periodontology 136:328–339
    [Google Scholar]
  13. Listgarten M. A., Hellden L. 1978; Relative distribution of bacteria at clinically healthy and periodontally diseased sites in humans. Journal of Clinical Periodontology 5:115–132
    [Google Scholar]
  14. Maltha J. C., Mikx F. H. M., Kuypers F. J. 1985; Necrotizing ulcerative gingivitis in beagle dogs. III. Distribution of spirochetes in interdental gingival tissue. Journal of Periodontal Research 20:522–531
    [Google Scholar]
  15. Mikx F. H. M., de Jong M. H. 1987; Keratinolytic activity of cutaneous and oral bacteria. Infection and Immunity 55:621–625
    [Google Scholar]
  16. Mikx F. H. M., Maltha J. C., Keulers R. A. C. 1989; Are there differences between oral Treponema in dental plaque and gingival tissue?. Journal of Dental Research 68: abstract 87
    [Google Scholar]
  17. Mikx F. H. M., Maltha J. C., van Campen G. J. 1990; Spirochetes in early lesion of necrotizing ulcerative gingivitis, experimentally induced in beagle dogs. Oral Microbiology and Immunology 5:86–89
    [Google Scholar]
  18. Ohta K., Makinen K. K., Loesche W. J. 1986; Purification and characterization of an enzyme produced by Treponema denticola capable of hydrolyzing synthetic trypsin substrates. Infection and Immunity 53:213–220
    [Google Scholar]
  19. Olson I. 1984; Attachment of Treponema denticola to cultured human epithelial cells. Scandinavian Journal of Dental Research 92:55–63
    [Google Scholar]
  20. Reijntjens F. M. J., Mikx F. H. M., Wolters Lutgerhorst J. M. L., Maltha J. C. 1986; Adherence of oral treponemas and their effect on morphological damage and detachment of epithelial cells in vitro . Infection and Immunity 51:642–647
    [Google Scholar]
  21. Sanna A., Dettori G., Grillo R., Rossi A., Chiarenza D. 1982; Isolation and propagation of a strain of Treponema from the human digestive tract - preliminary report. L’Igiene Moderna 77:287–297
    [Google Scholar]
  22. Sibo R., Al-Joburi W., Gornitsky M., Chan E. C. S. 1989; Synthesis and secretion of phospholipase C by oral spirochetes. Journal of Clinical Microbiology 27:568–570
    [Google Scholar]
  23. TER Steeg P. F., VAN der Hoeven J. S. 1990; Growth stimulation of Treponema denticola by periodontal microorganisms. Antonie van Leeuwenhoek 57:63–70 (abstract)
    [Google Scholar]
  24. ter Steeg P. F., van der Hoeven J. S., de Jong M. H., van Munster P. J., Jansen M. J. H. 1988; Modelling the gingival pocket by enrichment of subgingival microflora in human serum in chemostats. Microbial Ecology in Health and Disease 1:73–84
    [Google Scholar]
  25. Syed S. M., Salvador S. L., Loesche W. J. 1988; Enzyme profiles of oral spirochetes in Rap ID-ANA system. Journal of Clinical Microbiology 26:2226–2228
    [Google Scholar]
  26. Trevathan C. A., Smibert R. M., George H. A. 1982; Lipid catabolism of cultivated treponemas. Canadian Journal of Microbiology 28:672–678
    [Google Scholar]
  27. Uitto V. J., Chan E. C. S., Chin Quee T. 1986; Initial characterization of neutral proteinases from oral spirochetes. Journal of Periodontal Research 21:95–100
    [Google Scholar]
  28. Wahren A., Holme T. 1973; Amino acid and peptide requirement of Fusiformis necrophorus . Journal of Bacteriology 116:279–284
    [Google Scholar]
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