1887

Abstract

The gene from , encoding 5-dehydroquinate hydrolyase (3-dehydroquinase), has been cloned into and the DNA sequence determined. The gene was isolated from a cosmid gene bank by complementation of an mutant. Analysis of the DNA sequence revealed the presence of an open reading frame capable of encoding a protein of 252 amino acids with a calculated of 27706. Comparison of the deduced 3-dehydroquinase protein sequence with that elucidated for revealed 69 % homology. Alignment of the sequence and equivalent and sequences showed that homology was lower, at 24%, but still significant. Use of a minicell expression system demonstrated that a polyclonal antibody raised against 3-dehydroquinase cross-reacted with its counterpart.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-137-1-147
1991-01-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/137/1/mic-137-1-147.html?itemId=/content/journal/micro/10.1099/00221287-137-1-147&mimeType=html&fmt=ahah

References

  1. Bacon G. A., Burrows T. W., Yates M. 1951; The effects of biochemical mutation on the virulence of Bacterium typhosum : the loss of virulence of certain mutants. British Journal of Experimental Pathology 32:85–96
    [Google Scholar]
  2. Biggin M. D., Gibson T. G., Hong G. F. 1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences of the United States of America 803963–3965
    [Google Scholar]
  3. Carter P., Bedouelle H., Winter G. 1985; Improved oligonucleotide site directed mutagenesis using M 13 vectors. Nucleic Acids Research 13:4431–4443
    [Google Scholar]
  4. Catcheside D. E. A., Storer P. J., Klein B. 1985; Cloning of the ARO cluster gene of Neurospora crassa and its expression in Escherichia coli . Molecular and General Genetics 199:446–451
    [Google Scholar]
  5. Charles I. G., Keyte J. W., Brammar W. J., Hawkins A. R. 1985; Nucleotide sequence encoding the biosynthetic dehydroquinase function of the penta-functional AROM locus of Aspergillus nidulans . Nucleic Acids Research 13:8119–8128
    [Google Scholar]
  6. Charles I. G., Keyte J. W., Brammar W. J., Smith M., Hawkins A. R. 1986; The isolation and nucleotide sequence of the complex AROM locus of Aspergillus nidulans . Nucleic Acids Research 14:2201–2213
    [Google Scholar]
  7. Charles I. G., Lamb H. K., Pickard D., Dougan G., Hawkins A. R. 1990; Isolation, characterisation and nucleotide sequences of the aroC genes encoding chorismate synthase from Salmonella typhi and Escherichia coli . Journal of General Microbiology 136:353–358
    [Google Scholar]
  8. Da Silva A J. F., Whittington H., Clements J., Roberts C., Hawkins A. R. 1986; Sequence analysis and transformation by the catabolic 3-dehydroquinase (QUTE) gene from Aspergillus nidulans . Biochemical Journal 240:481–488
    [Google Scholar]
  9. Davis R. W., Botstein D., Roth J. R. 1980; Advanced Bacterial Genetics. A Manual for Genetic Engineering Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Dougan G., Sherratt D. J. 1977; Tna as a probe for the structure and function of ColEl. Molecular and General Genetics 151:151–160
    [Google Scholar]
  11. Dougan G., Maskell D., Pickard D., Hormaeche C. 1987; Isolation of stable aro A mutants of Salmonella typhi Ty2 : properties and preliminary characterisation in mice. Molecular and General Genetics 207:402–05
    [Google Scholar]
  12. Duncan K., Chaudhuri S., Campbell M. S., Coggins J. R. 1986; The over-expression and complete amino acid sequence of Escherichia coli 3-dehydroquinase. Biochemical Journal 238:475–483
    [Google Scholar]
  13. Duncan K., Edwards R. M., Coggins J. R. 1987; The pentafunctional AROM enzyme of Saccharomyces cerevisiae is a mosaic of monofunctional domains. Biochemical Journal 246:375–386
    [Google Scholar]
  14. Giles N. H. 1978; The organisation, function and evolution of gene clusters in eukaryotes. American Naturalist 112:641–657
    [Google Scholar]
  15. Giles N. H., Case M. F., Baum J., Geever R., Huiet L., Patel V., Tyler B. 1985; Gene organization and regulation in the QA (quinic acid) gene cluster of Neurospora crassa . Microbiology Reviews 49:338–358
    [Google Scholar]
  16. Grantham R., Gautier C, Gouy M., Jacobzone M., Mercier R. 1981; Codon catalogue usage is a genome strategy modulated for gene expressivity. Nucleic Acids Research 9:43–47
    [Google Scholar]
  17. Hawkins A. R. 1987; The complex AROM locus of Aspergillus nidulans: evidence for multigene fusions and convergent evolution. Current Genetics 11:491–498
    [Google Scholar]
  18. Hawkins A. R., Giles N. H., Kinghorn J. R. 1982; Genetical and biochemical aspects of quinate breakdown in the filamentous fungus Aspergillus nidulans . Biochemical Genetics 20:271–286
    [Google Scholar]
  19. Hohn B., Collins J. 1980; A small cosmid for efficient cloning of large DNA fragments. Gene 11:291–298
    [Google Scholar]
  20. Hoiseth S. K., Stocker B. A. D. 1981; Aromatic-dependent Salmonella typhimurium are non-virulent and are effective live vaccines. Nature, London 291:238–239
    [Google Scholar]
  21. Hull R. A., Gill R. E., Hsu P., Minshew B. H., Falkow S. 1981; Construction and expression of recombinant plasmids encoding Type 1 of D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infection and Immunity 33:933–938
    [Google Scholar]
  22. Kinghorn J. R., Hawkins A. R. 1982; Cloning and expression in Escherichia coli K 12 of the biosynthetic dehydroquinase function of the AROM cluster gene from the eukaryote Aspergillus nidulans . Molecular and General Genetics 186:145–152
    [Google Scholar]
  23. Kishore G. M., Shah D. M. 1988; Amino acid biosynthesis inhibitors as herbicides. Annual Review of Biochemistry 57:627–663
    [Google Scholar]
  24. Kushner S. R. 1978; An improved method for transformation of Escherichia coli with ColEl derived plasmids. In Genetic Engineering17–23 Boyer H. W., Nicosia S. Amsterdam: Elsevier/North-Holland Biomedicai Press;
    [Google Scholar]
  25. Levine M. M., Herrington D., Murphy J. R., Morris J. G., Losonsky G., Tall B., Linberg A. A., Svenson S., Baqar S., Frances-Edwards M., Stocker B. 1987; Safety, infectivity, immunogenicity, and in vivo stability of two attenuated auxotrophic mutant strains of Salmonella typhi 541Ty and 543Ty, as live oral vaccines in humans. Journal of Clinical Investigation 79:888–902
    [Google Scholar]
  26. Lumsden J., Coggins J. R. 1977; The subunit structure of the AROM multienzyme complex of Neurospora crassa A possible pentafunctional polypeptide chain. Biochemical Journal 161:599–607
    [Google Scholar]
  27. Maniatis R., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Maskell D. J., Morrissey P., Dougan G. 1988; The cloning and nucleotide sequence of the aroA gene of Bordetella pertussis . Journal of Bacteriology 1702467–2471
    [Google Scholar]
  29. Messing J., Vieira J. 1982; A new pair of M 13 vectors for selecting either DNA strand of double digested restriction fragments. Gene 19:269–276
    [Google Scholar]
  30. Miller J. M. 1972 Experiments in Molecular Genetics Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Miller I. A., Chatfield S., Dougan G., DeSilva L., Joysey H. S., Hormaeche C. E. 1989; Bacteriophage P22 as a vehicle for transducing cosmid gene banks between smooth strains of Salmonella typhimurium: use in identifying a role for aroD in attenuating virulent Salmonella strains. Molecular and General Genetics 215:312–316
    [Google Scholar]
  32. Morrissey P., Dougan G. 1986; Cloning and characterisation of the Escherichia coli 987P adhesion fimbriae determinant. Gene 43:79–84
    [Google Scholar]
  33. O’Callaghan D., Maskell D., Liew F. Y., Easmon C. S., Dougan G. 1988; Characterisation of aromatic and purine dependent Salmonella typhimurium: attenuation, persistence and ability to induce protective immunity in BALB/c mice. Journal of Bacteriology 56:419–423
    [Google Scholar]
  34. Pittard A. J. 1987; Biosynthesis of the aromatic amino acids. In Escherichia coli and Salmonella typhimurium. Cellular and Molecular Biology Neidhardt F. C. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  35. Polley L. D. 1978; Purification and characterization of 3-dehydroquinate hydrolase and shikimate oxidoreductase, evidence for a bifunctional enzyme. Biochemica et Biophysica Acta 526:259–266
    [Google Scholar]
  36. Rosenberg M., Court M. 1979; Regulatory sequences involved in the promotion and termination of RN A transcription. Annual Review of Genetics 13:319–353
    [Google Scholar]
  37. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 715463–5467
    [Google Scholar]
  38. Shine J., Dalgarno L. 1974; The 3′ terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proceedings of the National Academy of Sciences of the United States of America 711342–1346
    [Google Scholar]
  39. Staden R. 1982; An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Research 10:2951–2961
    [Google Scholar]
  40. Strauss E. C, Kobori J. A., Siu G., Hood L. E. 1986; Specific-primer-directed DNA sequencing. Analytical Biochemistry 154:353–360
    [Google Scholar]
  41. Tabor S., Richardson C. C. 1987; DNA sequence analysis with a modified bacteriophage T7 polymerase. Proceedings of the National Academy of Sciences of the United States of America 844767–4771
    [Google Scholar]
  42. Tautz D., Renz M. 1983; An optimised freeze-squeeze method for the recovery of DNA fragments from agarose gels. Analytical Biochemistry 132:14–19
    [Google Scholar]
  43. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 764350–4354
    [Google Scholar]
  44. Ubben D., Schmitt R. 1986; Tn1721 derivatives for transposon mutagenesis, restriction mapping and nucleotide sequence analysis. GeneAX145–152
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-137-1-147
Loading
/content/journal/micro/10.1099/00221287-137-1-147
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error