Summary: The gene from , encoding 5-dehydroquinate hydrolyase (3-dehydroquinase), has been cloned into and the DNA sequence determined. The gene was isolated from a cosmid gene bank by complementation of an mutant. Analysis of the DNA sequence revealed the presence of an open reading frame capable of encoding a protein of 252 amino acids with a calculated of 27706. Comparison of the deduced 3-dehydroquinase protein sequence with that elucidated for revealed 69% homology. Alignment of the sequence and equivalent and sequences showed that homology was lower, at 24%, but still significant. Use of a minicell expression system demonstrated that a polyclonal antibody raised against 3-dehydroquinase cross-reacted with its counterpart.


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