1887

Abstract

Summary: Engineered variants of the transposon Tn have been widely used to obtain insertion mutations and transcriptional fusions in and other Gram-positive bacteria. We have developed a novel Tn-based methodology useful for isolation and characterization of mutants resulting from gene over-expression. A Tn variant was constructed which contains a strong out-facing promoter near one end, able to promote transcription of genes in the vicinity of its insertion target. This transposon, designated Tn, was tested in model conditions. Three Tn mutants of , with phenotypes presumed to result from gene over-expression, were analysed. Their phenotypes were shown to be due to transcription from the transposon promoter. In one mutant the promoter activated a gene, probably . The other two contained different insertions decryptifying a gene encoding -galactosidase.

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1991-01-01
2021-09-24
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