%0 Journal Article %A Hiraga, Kazumi %A Tahara, Hidetaka %A Taguchi, Nobuhisa %A Tsuchiya, Eiko %A Fukui, Sakuzo %A Miyakawa, Tokichi %T Inhibition of membrane Ca2+-ATPase of Saccharomyces cerevisiae by mating pheromone α-factor in vitro %D 1991 %J Microbiology, %V 137 %N 1 %P 1-4 %@ 1465-2080 %R https://doi.org/10.1099/00221287-137-1-1 %I Microbiology Society, %X Summary Plasma membrane Ca2+-ATPase of Saccharomyces cerevisiae was solubilized and partially purified by calmodulin-affinity chromatography. The activity of Ca2+-ATPase isolated from MATa cells was inhibited by physiological concentrations of the mating pheromone α-factor in a dose-dependent manner. The enzyme prepared from a receptor-deficient sterile mutant cells (Δste2) was similarly inhibited by α-factor, but the enzyme from MATα cells was resistant to the mating pheromone. We suggest that the inhibition may be involved in the α-factor-induced increase of Ca2+ uptake reaction of MATa cells. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-137-1-1