RT Journal Article SR Electronic(1) A1 Rogall, Till A1 Flohr, Thomas A1 Böttger, Erik C.YR 1990 T1 Differentiation of Mycobacterium species by direct sequencing of amplified DNA JF Microbiology, VO 136 IS 9 SP 1915 OP 1920 DO https://doi.org/10.1099/00221287-136-9-1915 PB Microbiology Society, SN 1465-2080, AB Nucleotide sequences specific for a range of Mycobacterium species were defined by computer-assisted sequence comparisons of small subunit ribosomal RNA. A polymerase chain reaction-based sequencing strategy was used to demonstrate that the 16S rRNA sequence can be used for the rapid identification of mycobacterial isolates. Identification at the species level can be obtained within 2 d, requiring < 10000 bacteria. This procedure reliably differentiates Mycobacterium spp. which are difficult to identify by classical methods, such as M. malmoense, M. szulgai and M. flavescens., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-9-1915