1887

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Volume 136, Issue 9

Biosynthesis of anthracyclines: analysis of mutants of sp. strain C5 blocked in daunomycin biosynthesis Free

Abstract

sp. strain C5, an organism that normally produces baumycins, daunomycin and ε-rhodomycinone, was treaed with -methyl-′-nitro--nitrosoguanidine (NTG). Mutants blocked at arious points in daunomycin and baumycin production were isolated by screening for altered pigmentation and absnce of bioactivity against . Examination of the mutants by thin-layer chromatography of their accumulated anthracycline metabolites, by cosynthesis assays, and by extract feeding experiments allowed a classification into six groups. Theses were: , strains that accumulated no anthracyclines but with other blocked mutants cosynthesized anthracyclines (polyketide-synthase-minus mutants); , regulatory mutants that, either alone or mixed with other blocked mutants, accumulated no anthracyclines; , mutants that accumulated aklanonic acid; , mutants that accumulated maggiemycin; , mutants that accumulated aklavinone; and , mutants that accumulated only ε-rhodomycinone. Mutant SC5-24 (), which accumulated that shunt product maggiemycin, was re-mutagenized with NTG to obtain blocked mutants in preceding biosynthetic steps; the three grops of double mutants obtained accumulated aklanonic acid (), aklanonic acid methyl ester () and akalviketone ().

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
© Society For General Microbiology 1990
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1990-09-01
2024-04-24
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Biosynthesis of anthracyclines: analysis of mutants of Streptomyces sp. strain C5 blocked in daunomycin biosynthesis
Microbiology 136, 1877 (1990); https://doi.org/10.1099/00221287-136-9-1877
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