1887

Abstract

Summary: sp. strain C5, an organism that normally produces baumycins, daunomycin and ɛ-rhodomycinone, was treaed with N-methyl-N'-nitro-N-nitrosoguanidine (NTG). Mutants blocked at arious points in daunomycin and baumycin production were isolated by screening for altered pigmentation and absnce of bioactivity against . Examination of the mutants by thin-layer chromatography of their accumulated anthracycline metabolites, by cosynthesis assays, and by extract feeding experiments allowed a classification into six groups. Theses were: , strains that accumulated no anthracyclines but with other blocked mutants cosynthesized anthracyclines (polyketide-synthase-minus mutants); , regulatory mutants that, either alone or mixed with other blocked mutants, accumulated no anthracyclines; , mutants that accumulated aklanonic acid; , mutants that accumulated maggiemycin; , mutants that accumulated aklavinone; and , mutants that accumulated only ɛ-rhodomycinone. Mutant SC5-24 (), which accumulated that shunt product maggiemycin, was re-mutagenized with NTG to obtain blocked mutants in preceding biosynthetic steps; the three grops of double mutants obtained accumulated aklanonic acid (), aklanonic acid methyl ester () and akalviketone ().

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-9-1877
1990-09-01
2019-10-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-9-1877
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error