Acquisition of iron from citrate by Free

Abstract

Transport of [C]citrate, ferric [C]citrate and [Fe]ferric citrate into grown in synthetic media containing citrate, succinate, or succinate and citrate as carbon and energy sources was measured. Cells grown in citrate-containing medium transported radiolabeled citrate and iron, whereas the succinate-grown cells transported iron but not citrate. Binding studies revealed that isolated outer and inner membranes of citrate-grown cells contain a citrate receptor, absent from membranes of succinate-grown cells. [Fe]Ferric citrate bound to the isolated outer membranes of each cell type. The failure of citrate to compete with this binding suggests the presence of a ferric citrate receptor on the outer membranes of each cell type. Citrate induced the synthesis of two outer-membrane proteins of 41 and 19 kDa. A third protein of 17 kDa was more dominant in citrate-grown cells than in succinate-grown cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-9-1859
1990-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/9/mic-136-9-1859.html?itemId=/content/journal/micro/10.1099/00221287-136-9-1859&mimeType=html&fmt=ahah

References

  1. Ankenbauer R., Sriyosachati S., Cox C. D. 1985; Effects of siderophores on the growth of Pseudomonas aeruginosa in human serum and transferrin. Infection and Immunity 49:132–140
    [Google Scholar]
  2. Archibald R. S., De Voe I. W. 1980; Iron acquisition by Neisseria meningitidis. Infection and Immunity 27:322–334
    [Google Scholar]
  3. Braun V. 1985; The iron transport systems of Escherichia coli. In The Enzymes of Biological Membranes pp. 617–652 Martonosi A. N. Edited by New York: Plenum Press;
    [Google Scholar]
  4. Cox C. D. 1980; Iron uptake with ferripyochelin and ferric citrate by Pseudomonas aeruginosa. Journal of Bacteriology 142581–587
    [Google Scholar]
  5. Frost G. E., Rosenberg H. 1973; The inducible citrate-dependent iron transport system in Escherichia coli K- 12. Biochimica et Biophysica Acta 300:90–101
    [Google Scholar]
  6. Hohnadel D., Meyer J. M. 1988; Specificity of pyoverdine-mediated iron uptake among fluorescent Pseudomonas strains. Journal of Bacteriology 170:4865–4873
    [Google Scholar]
  7. Hussein S., Hantke K., Braun V. 1981; Citrate-dependent iron transport system in E. coli K-12. European Journal of Biochemistry 117:431–437
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  9. Lawford H. G., Williams G. R. 1971; The transport of citrate and other tricarboxylic acids in two species of Pseudomonas. Biochemical Journal 123:571–577
    [Google Scholar]
  10. Messenger A.J.M., Ratledge C. 1982; Iron transport in Mycobacterium smegmatis: uptake of iron from ferric citrate. Journal of Bacteriology 149:131–135
    [Google Scholar]
  11. Meyer J. M., Abdallah M. A. 1978; The fluorescent pigment of Pseudomonas fluorescens : biosynthesis, purification and physicochemical properties. Journal of General Microbiology 107:319–328
    [Google Scholar]
  12. Meyer J. M., Mock M., Abdallah M. A. 1979; Effect of iron on the protein composition of the outer membrane of fluorescent pseudomonads. FEMS Microbiology Letters 5:395–398
    [Google Scholar]
  13. Meyer J. M., Halle F., Hohnadel D., Lemanceau P., Ratefiarivelo H. 1987; Siderophores of Pseudomonas - biological properties. In Iron Transport in Microbes, Plants, and Animals pp. 189–205 Winkelmann G., Van der Helm D., Neilands J. B. Edited by Weinheim: VCH Verlagsgesellschaft;
    [Google Scholar]
  14. Mizuno T., Kageyama M. 1978; Separation and characterization of the outer membranes of Pseudomonas aeruginosa. Journal of Biochemistry 84:179–191
    [Google Scholar]
  15. Simonson C., Trivett T., Devoe I. W. 1981; Energy-dependent uptake of iron from citrate by isolated outer membranes of Neisseria meningitidis. Infection and Immunity 31:547–553
    [Google Scholar]
  16. Sokol P. A., Woods D. E. 1983; Demonstration of an iron-siderophore binding protein in the outer membrane of Pseudomonas aeruginosa. Infection and Immunity 40:665–669
    [Google Scholar]
  17. Spiro T. G., Pape L., Saltman P. 1967a; The hydrolytic polymerization of ferric citrate. I. The chemistry of the polymer. Journal of the American Chemical Society 89:5555–5559
    [Google Scholar]
  18. Spiro T. G., Pape L., Saltman P. 1967b; The hydrolytic polymerization of ferric citrate. II. The influence of excess, citrate. Journal of the American Chemical Society 89:5559–5562
    [Google Scholar]
  19. Wagegg W., Braun V. 1981; Ferric citrate transport in Escherichia coli requires outer membrane receptor protein FecA. Journal of Bacteriology 145:156–163
    [Google Scholar]
  20. Weissbach A., Hurwitz J. 1959; The formation of 2-keto-3- deoxyheptonic acid in extracts of Escherichia coli B. Journal of Biological Chemistry 234:705–709
    [Google Scholar]
  21. Winkelmann G. 1979; Surface iron polymers and hydroxy acids. A model of iron supply to sideramine-free fungi. Archives of Microbiology 121:43–51
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-9-1859
Loading
/content/journal/micro/10.1099/00221287-136-9-1859
Loading

Data & Media loading...

Most cited Most Cited RSS feed