1887

Abstract

Summary: Transport of [C]citrate, ferric [C]citrate and [55Fe]ferric citrate into grown in synthetic media containing citrate, succinate, or succinate and citrate as carbon and energy sources was measured. Cells grown in citrate-containing medium transported radiolabeled citrate and iron, whereas the succinate-grown cells transported iron but not citrate. Binding studies revealed that isolated outer and inner membranes of citrate-grown cells contain a citrate receptor, absent from membranes of succinate-grown cells. [55Fe]Ferric citrate bound to the isolated outer membranes of each cell type. The failure of citrate to compete with this binding suggests the presence of a ferric citrate receptor on the outer membranes of each cell type. Citrate induced the synthesis of two outer-membrane proteins of 41 and 19 kDa. A third protein of 17 kDa was more dominant in citrate-grown cells than in succinate-grown cells.

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/content/journal/micro/10.1099/00221287-136-9-1859
1990-09-01
2020-01-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-9-1859
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