1887

Abstract

The mutations of and decrease plasma-membrane ATPase activity. This study investigated how they affect different stress tolerances, and the extent and duration of the heat-shock response. mutants exhibited higher resistance to ethanol and osmotic stress, but lower tolerance to ultraviolet damage, as compared to wild-type cells. mutations also increased tolerance of the lethal temperature of 48 °C in cells in which no heat-shock response had been induced. However, after induction of a heat-shock response and elevated thermotolerance by a 25–38 °C upshift, then maintaining cells at 38 °C for 40 min, lowered subsequent tolerances of much higher lethal temperatures. Analysis of pulse-labelled proteins revealed reduced heat-shock protein synthesis in the mutant after a 25–38 °C heat shock. This may explain the greater increases in thermotolerance in wild-type as compared to cells after both were given identical 25–38 °C shocks. With more severe treatment (25–42 °C), heat-shock protein synthesis in wild-type cells, although initially high, was switched off more rapidly than in the mutant. These results indicate that plasma-membrane ATPase action exerts a major influence over several stress tolerances, as well as the extent and duration of heat-shock protein synthesis following induction of the heat-shock response.

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1990-09-01
2021-10-16
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