Summary: Thirty-three neomycin- and temperature-sensitive mutants of were isolated and characterized. The mutations fell into 29 complementation groups of omycin sensitive mutations (). At the restrictive temperature (37 °C), ten mutants arrested at specific stages in the cell division cycle: four at early G1 stage, two at late G1 stage, one at S phase, and three at G2 stage. None of the G1-arresting mutants isolated in this experiment appeared to be allelic with mutants examined, suggesting that these are new mutants. The mutants were further characterized by determining their cross-sensitivity to G418, cycloheximide, LiCl and CaCl.


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