1887

Abstract

Batch cultures of a brewer’s strain of , NCYC 227, in a defined medium exhibited characteristic flocculation during the late exponential phase of growth. However, early exponential cells were non-flocculent and flocculation of such cells could not be induced even in the presence of Ca. A specific glycoprotein, absent from the cell walls of non-flocculating exponentially growing cells of this yeast and those of non-flocculating strains of , was identified on the cell wall surface of flocculating exponentially growing cells of NCYC 227. Flocculent cells of NCYC 227 dispersed with EDTA and coated with monovalent Fab portions of the antibody showed reduced flocculation. Removal of the monovalent antibody portions from the cell surfaces induced cell flocculation in the presence of Ca. These results suggest a role for this glycoprotein in yeast cell flocculation.

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1990-09-01
2022-01-16
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References

  1. Amri M. A., Bonaly R., Duteurtre B., Moll M. 1982; Yeast flocculation: influence of nutritional factors on cell wall composition. Journal of General Microbiology 128:2001–2009
    [Google Scholar]
  2. Beavan M. J., Belki D., Stewart G. G., Rose A. H. 1979; Changes in electrophoretic mobility and lytic enzyme activity associated with developments of flocculating ability in Saccharomyces cerevisiae. Canadian Journal of Microbiology 25:888–895
    [Google Scholar]
  3. Calleja G. B. 1987; Cell aggregation. In The Yeasts, 2 pp 165–237 Rose A. H. Edited by London: Academic Press;
    [Google Scholar]
  4. Campbell I., Brudzynski A. 1966; Serological studies on brewing yeasts. Journal of the Institute of Brewing 72:556–560
    [Google Scholar]
  5. Campbell I., Robson F. O., Hough J. S. 1968; Serological investigations of fining and flocculent yeasts. Journal of the Institute of Brewing 74:360–364
    [Google Scholar]
  6. Cawley T. N., Ballou C. E. 1972; Identification of two Saccharomyces cerevisiae cell wall mannanchemotypes. Journal of Bacteriology 111:690–695
    [Google Scholar]
  7. Harris J. O. 1959; Possible mechanism of yeast flocculation. Journal of the Institute of Brewing 65:5–6
    [Google Scholar]
  8. Jayatissa P. M., Rose A. H. 1976; Role of phosphomannan in flocculation of Saccharomyces cerevisiae. Journal of General Microbiology 96:165–174
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Lyons T. P., Hough J. S. 1970; Flocculation of brewer’s yeast. Journal of the Institute of Brewing 76:564–571
    [Google Scholar]
  11. Lyons T. P., Hough J. S. 1971; Further evidence for the cross bridging hypothesis for flocculation of brewer’s yeast. Journal of the Institute of Brewing 77:300–305
    [Google Scholar]
  12. Marfey P., Sorensen S. B., Ottensen M. 1977; Studies on yeast flocculation. Comparison of enzymatic digests of flocculent and non flocculent cells of Saccharomyces carlsbergensis. Carlsberg Research Communications 42:353–367
    [Google Scholar]
  13. Miki B.L.A., Poon N. H., James A. P., Seligy V. L. 1981; Flocculation in Saccharomyces cerevisiae: mechanism of cell-cell interactions. In Current Developments in Yeast Research, pp 193–198 Stewart G. G., Russell I. Edited by Toronto: Pergamon Press;
    [Google Scholar]
  14. Miki B.L.A., Poon N. H., James A. P., Seligy V. L. 1982; Possible mechanism for flocculation interactions governed by gene FLOl in Saccharomyces cerevisiae. Journal of Bacteriology 150:878–889
    [Google Scholar]
  15. Mill P. J. 1964; The nature of interactions between flocculent cells in the flocculation of Saccharomyces cerevisiae. Journal of General Microbiology 35:61–68
    [Google Scholar]
  16. Phaff H. J. 1971; Structure and biosynthesis of the yeast cell envelope. In The Yeasts, 2 pp 135–210 Rose A. H., Harrison J. S. Edited by New York & London: Academic Press;
    [Google Scholar]
  17. Porter R. R. 1959; The hydrolysis of rabbit γ-globulin and antibodies with crystalline papain. Biochemical Journal 73:119–126
    [Google Scholar]
  18. Rose A. H. 1984; Physiology of cell aggregation: flocculation by Saccharomyces cerevisiae as a model system. In Microbial Adhesion and Aggregation, pp 323–335 Marshall K. C. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  19. Rosenfeld L., Ballou C. E. 1972; Genetic control of yeast mannan structure (biological basis for the transformation of S. cerevisiae somatic antigen). Journal of Biological Chemistry 249:2319–2321
    [Google Scholar]
  20. Stewart G. G. 1975; Yeast flocculation. Brewers Digest 50:42–57
    [Google Scholar]
  21. Stewart G. G., Goring T. E. 1976; Effect of some monovalent and divalent metal ions on the flocculation of brewer’s yeast strains. Journal of the Institute of Brewing 82:341–342
    [Google Scholar]
  22. Stewart G. G., Russell I., Garrison I. F. 1975; Some considerations of the flocculation characteristics of ale and lager yeast strains. Journal of the Institute of Brewing 81:248–257
    [Google Scholar]
  23. Taylor N. W., Orton W. L. 1978; Aromatic components and sugars in flocculation of Saccharomyces cerevisiae. Journal of the Institute of Brewing 84:113–114
    [Google Scholar]
  24. Tijssen P. 1985; Practice and theory of immunoassays. In Laboratory Techniques in Biochemistry and Molecular Biology, 15 p. 549 Amsterdam, New York & Oxford: Elsevier;
    [Google Scholar]
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