1887

Abstract

A chromosome assay method was used to determine the heterokaryon compatibility relationships between strains belonging to heterokaryon-compatibility (h-c) groups A and G1 of A hybrid strain (RD15) was isolated following protoplast fusion of strains 65–5 (h-cA) and 7–141 (h-cG1). The morphology of RD15 was severely abnormal compared to diploid strains of produced from heterokaryon-compatible haploid parents. Inocula of RD15 were induced to haploidize on medium containing Benlate and a parasexual progeny sample of 291 haploid segregants was obtained. The progeny strains were genotyped for standard markers. Allelic ratios and pairwise marker segregations were determined. Pairs of progeny strains that carried different alleles for the standard markers on each linkage group in turn were tested for compatibility. Strain pairs that possessed different alleles for the markers on linkage groups II, III, V, VI and VII were incompatible indicating the presence of heterokaryon-incompatible () genes on these linkage groups. Backcrosses to an h-cGl strain showed that two were located on linkage group III and confirmed a total of six gene differences between the h-cA and h-cGl strains.

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1990-09-01
2024-03-28
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References

  1. Butcher A. C. 1968; The relationship between sexual outcrossing and heterokaryon incompatibility in Aspergillus nidulans. Heredity 23:443–452
    [Google Scholar]
  2. Caten C., Jinks J. L. 1966; Heterokaryosis: its significance in wild homothallic Ascomycetes and Fungi Imperfecti. Transactions of the British Mycological Society 49:81–93
    [Google Scholar]
  3. Clutterbuck A. J. 1974 Aspergillus nidulans In Handbook of Genetics, 1 pp 447–510 King R. C. Edited by New York: Plenum Press;
    [Google Scholar]
  4. Croft J. H., Jinks J. L. 1977; Aspects of the population genetics of Aspergillus nidulans. In Genetics and Physiology of Aspergillus, pp 339–360 Smith J. E., Pateman J. A. Edited by London: Academic Press;
    [Google Scholar]
  5. Dales R.B.G., Croft J. H. 1977; Protoplast fusion and the isolation of heterokaryons and diploids from vegetatively incompatible strains of Aspergillus nidulans. FEMS Microbiology Letters 1:201–203
    [Google Scholar]
  6. Dales R.B.G., Croft J. H. 1983; A chromosome assay method for the detection of heterokaryon incompatibility (het) genes operating between members of different heterokaryon compatibility (h-c) groups in Aspergillus nidulans. Journal of General Microbiology 129:3643–3649
    [Google Scholar]
  7. Dales R.B.G., Moorhouse J., Croft J. H. 1983; The location and analysis of two heterokaryon incompatibility (het) loci in strains of Aspergillus nidulans. Journal of General Microbiology 129:3637–3642
    [Google Scholar]
  8. Grindle M. 1963a; Heterokaryon compatibility of unrelated strains in the Aspergillus nidulans group. Heredity 18:191–204
    [Google Scholar]
  9. Grindle M. 1963b; Heterokaryon compatibility of closely related wild isolates of Aspergillus nidulans. Heredity 18:397–405
    [Google Scholar]
  10. Jinks J. L., Caten C. E., Simchen G., Croft J. H. 1966; Heterokaryon incompatibility and variation in wild populations of Aspergillus nidulans. Heredity 21:227–239
    [Google Scholar]
  11. Káfer E. 1961; The process of spontaneous recombination in vegetative nuclei of Aspergillus nidulans. Genetics 46:1581–1609
    [Google Scholar]
  12. Káfer E. 1977; Meiotic and mitotic recombination in Aspergillusand its chromosomal aberrations. Advances in Genetics 19:33–131
    [Google Scholar]
  13. McCully K. S., Forbes E. 1965; The use of p-fluorophenylalanine with ‘master strains’ of Aspergillus nidulans for assigning genes to linkage groups. Genetical Research 6:352–359
    [Google Scholar]
  14. Pollard R., Käfer E., Johnston M. 1968; Influence of chromosomal aberrations on meiotic and mitotic non-disjunction in Aspergillus nidulans. Genetics 60:743–757
    [Google Scholar]
  15. Pontecorvo G., Roper J. A., Hemmons L. M., MacDonald K. D., Bufton A.W.J. 1953; The genetics of Aspergillus nidulans. Advances in Genetics 5:141–238
    [Google Scholar]
  16. Roper J. A. 1966; Mechanisms of inheritance: the parasexual cycle. In The Fungi, 2 pp 589–617 Ainsworth G. C., Sussman A. S. Edited by New York: Academic Press;
    [Google Scholar]
  17. Thom C., Church M. 1926 The Aspergilli. Baltimore:: Williams & Wilkins.;
    [Google Scholar]
  18. Thom C., Raper K. B. 1945 A Manual of the Aspergilli. Baltimore:: Williams & Wilkins.;
    [Google Scholar]
  19. Upshall A., Käfer E. 1974; Detection and identification of translocations by increased specific non-disjunction in Aspergillus nidulans. Genetics 16:19–31
    [Google Scholar]
  20. Upshall A., Giddings B., Mortimore I. D. 1977; The use of Benlate for distinguishing between haploid and diploid strains of Aspergillus nidulans and Aspergillus terreus. . Journal of General Microbiology 100:413–418
    [Google Scholar]
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