1887

Abstract

Ribosomal DNA (rDNA) polymorphism was compared with electrophoretic enzyme polymorphism for the intra-and interspecies differentiation of and DNA from 90 strains previously classified into six zymotypes ( and ) and into distinct enzyme electrophoretic patterns (the four other species) was digested with RI or dIII and analysed by Southern blotting. The six species were clearly differentiated from each other. In , the subclassification of biotype 1 into zymotypes 1A and 1B was also reflected in the rDNA and the four other bio-zymotypes gave four different classes of restriction pattern. In , both RI and III gave five distinct riboclasses which correlated with the zymotypes. In the four other species, the phenotype polymorphism appeared to be better correlated with the restriction fragment length polymorphism data in some enzymes than others. The data demonstrate that the inter- and intraspecies classification by rDNA polymorphism using two restriction enzymes is similar to that based on electrophoretic enzyme polymorphism. The analysis could be refined for taxonomic and epidemiological purposes by using other restriction enzymes.

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1990-08-01
2021-10-19
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References

  1. Allardet-Servent A., Bourg G., Ramuz M., Pages M., Bellis M. 1988; DNA polymorphism in strains of the genus Brucella . Journal of Bacteriology 170:4603–4607
    [Google Scholar]
  2. Bercovier H., Kafri O., Sela S. 1986; Mycobacteria possess a surprisingly small number of ribosomal RN A genes in relation to the size of their genome. Biochemical and Biophysical Research Communications 136:1136–1141
    [Google Scholar]
  3. Brenner D.J., Ursing J., Bercovier H., Steigerwalt A.G., Fanning G.R., Carter G.P., Mollaret H.H. 1980; Deoxyribonucleic acid relatedness in Yersinia enterocoliticaand Yersinia enterocolitica-Wktorganisms. Current Microbiology 4:195–200
    [Google Scholar]
  4. Denny T.P., Gilmour M.N., Selander R.K. 1988; Genetic diversity and relationships of two pathovars of Pseudomonas syringae . Journal of General Microbiology 134:1949–1960
    [Google Scholar]
  5. Goullet PH., Picard B. 1984; Distinctive electrophoretic and isoelectric focusing patterns of esterases from Yersinia enterocolitica and Yersinia pseudotuberculosis . Journal of General Microbiology 130:1471–1480
    [Google Scholar]
  6. Goullet PH., Picard B. 1988; Characterization of Yersinia enterocolitica, Y. intermedia, Y. aldovae, Y. frederiksenii, Y. kristenseniiand Y. pseudotuberculosisby electrophoretic polymorphism of acid phosphatase, esterases, and glutamate and malate dehydrogen-ases. Journal of General Microbiology 134:317–325
    [Google Scholar]
  7. Grimont F., Grimont P.A.D. 1986; Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Annales de I’Institut Pasteur/Microbiologie 137B:165–175
    [Google Scholar]
  8. Irino K., Grimont F., Casan I., Grimont P.A.D. The Braziliapnu Rpuricfe Fever Study Group 1988; rRNA gene restriction patterns of Haemophilus influenzaebiogroup aegyptius strains associated with Brazilian purpuric fever. Journal of Clinical Microbiology 26:1535–1538
    [Google Scholar]
  9. Kakoyiannis C.K., Winter P.J., Marshall R.B. 1984; Identification of Campylobacter coliisolates from animals and humans by bacterial restriction endonuclease DNA analysis. Applied and Environmental Microbiology 48:545–549
    [Google Scholar]
  10. Kristiansen B.E., Sorensen B., Spanne O., Bjorvatn B. 1985; Restriction fingerprinting and serology in a small outbreak of B15 meningococcal disease among Norwegian soldiers. Scandinavian Journal of Infectious Diseases 17:19–24
    [Google Scholar]
  11. Kuijper E.J., Van alphen L., Leenders E., Zanen H.C. 1989; Typing of Aeromonas strains by DNA restriction endonuclease analysis and polyacrylamide gel electrophoresis of cell envelopes. Journal of Clinical Microbiology 27:1280–1285
    [Google Scholar]
  12. Mcclenaghan M., Herring A.J., Aitken I.D. 1984; Comparison of Chlamydia psittaciisolates by DNA restriction endonuclease analysis. Infection and Immunity 45:384–389
    [Google Scholar]
  13. Magee B.B., D’Souza T.M., Magee P.T. 1987; Strain and species identification by restriction fragment length polymorphisms in the ribosomal DNA repeat of Candida species. Journal of Bacteriology 169:1639–1643
    [Google Scholar]
  14. Marshall R.B., Winter P.J., Yanagawa Y. 1984; Restriction endonuclease DNA analysis of Leptospira interrogansserovars icterohaemorrhagiaeand hebdomadis . Journal of Clinical Microbiology 20:808–810
    [Google Scholar]
  15. Patterson T.F., Patterson J.E., Masecar B.L., Barden G.E., Hierholzer W.J., Zervos M.J. 1988; A nosocomial outbreak of Branhamella catarrhalisconfirmed by restriction endonuclease analysis. Journal of Infectious Diseases 157:996–1001
    [Google Scholar]
  16. Picard-Pasquier N., Ouaghed M., Picard B., Goullet PH., Krishnamoorthy R. 1989; A simple, sensitive method of analyzing bacterial ribosomal DNA polymorphism. Electrophoresis 10:186–189
    [Google Scholar]
  17. Schwartz D.C., Cantor C.R. 1984; Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell 37:67–75
    [Google Scholar]
  18. Southern E.M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  19. Stull T.L., Lipuma J.J., Edlind T.D. 1988; A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. Journal of Infectious Diseases 157:280–286
    [Google Scholar]
  20. Ursing J., Brenner D.J., Bercovier H., Fanning R., Steiger-Walt A.G., Brault J., Mollaret H.H. 1980; Yersinia frederiksenii:a new species of Enterobacteriaceae composed of rhamnose-positive strains (formerly called atypical Yersinia enteroco- liticaor Yersinia enterocolitica-likt) . Current Microbiology 4:213–217
    [Google Scholar]
  21. Yogev D., Halachmi D., Kenny G.E., Razin S. 1988; Distinction of species and strains of mycoplasmas (mollicutes) by genomic DNA fingerprints with an rRNA gene probe. Journal of Clinical Microbiology 26:1198–1201
    [Google Scholar]
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