Summary: The gene, coding for a glucosyltransferase which synthesizes water-soluble glucan and previously cloned from strain MFe28 (mutans serotype ) into a bacteriophage vector, was subcloned into a plasmid vector. The gene products expressed in were compared to the primer-independent, oligoisomaltosaccharide synthase in strain AHT (mutans serotype ) and shown to resemble it closely in molecular mass, isoelectric point, immunological properties, optimum pH and values. The glucans produced from sucrose by the gene products are α-1,6-linked linear oligo-isomaltosaccharides without any branching sites. A similar gene was also detected on chromosomal DNA from strain AHT.


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